Immunocytochemical study of the plasma membrane and the Cytoskeleton of the transitional epithelium.

质膜和移行上皮细胞骨架的免疫细胞化学研究。

• 批准号: 61480092
• 负责人: SEGUCHI Harumichi
• 金额: $ 4.16万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480092/
• 关键词: Transitional epithelium; Cytoskeleton; Immunohistochemistry; Rapid-freeze-deep-etching method; Actin filament; アクチンフィラメント; ケラチンフィラメント; 酵素細胞化学; ライソゾーム; 骨細骨格; 微細線維; 移行上皮細胞; 急速凍結固定; ディープエッチング法; 非対称性単位膜; 免疫組織細胞化学; 抗中間径フィラメント抗体; 抗アクチン抗体; 抗ミオシ

We have investigated the ultrastructural and biochemical characteristics of the cytoskeleton, and the relationship between the luminal plasma membrane and cytoskeleton in the transitional epithelial cells employed immunohistochemistry and rapid-freeze-deep-etching replicas. The actin filaments, reacted with anti-actin antibody and heavy meromyosin, existed just beneath the luminal plasma membrane. These filaments attached to the luminal plasma membrane and formed meshwork. They also attached to fusiform vesicles and mitochondria. Keratin filament which reacted with anti-keratin antibody ran parallel each other forming bundles in the cytoplasm of transitional epithelial ells. They never attached directly to the luminal plasma membrane. We never found the filaments which reacted with anti-vimentin or anti-desmin antibody in the transition epithelium. In order to know the fate of the luminal plasma membrane and the filaments in this epithelium, we have studied the ultrastructural localization of acid phosphatase(ACPasa) and trimetaphosphatase(TMPase) activities used as marker enzymes of lysosomes. Most of lysosmes were ACPase positive a few of them were TMPase positive. To clarify the relationship between these two kind of lysosomes further investigation are neccessary.

我们用免疫组织化学和快速冷冻深蚀刻复制法研究了移行上皮细胞的细胞骨架的超微结构和生化特征，以及管腔质膜和细胞骨架之间的关系。肌动蛋白细丝与抗肌动蛋白抗体和重肌球蛋白反应，仅存在于管腔质膜下方。这些细丝附着在管腔质膜上，形成网状结构。它们还附着在纺锤形小泡和线粒体上。与抗角蛋白抗体反应的角蛋白细丝平行排列，在移行上皮细胞胞浆内形成束状排列。它们从未直接附着在管腔质膜上。在移行上皮中未见与波形蛋白或结蛋白抗体反应的细丝。为了了解管腔质膜和管丝在该上皮中的去向，我们研究了溶酶体标志酶酸性磷酸酶(ACPasa)和三偏磷酸酶(TMPase)的超微结构定位。大部分溶酶呈ACPase阳性，少数为TMPase阳性。为了弄清这两种溶酶体之间的关系，有必要进行进一步的研究。

项目成果

瀬口春道: 皮膚病未療. 10. 493-501 (1988)

Harumichi Seguchi：未经治疗的皮肤病。10. 493-501 (1988)

T.Inomata: Radiother.Syst.Res.5. 229-232 (1988)

T.Inomata：Radiother.Syst.Res.5。

Y.Ogawa: Rediother.Syst.Res.5. 233-236 (1988)

Y.Okawa：Rediother.Syst.Res.5。

小川恭弘: 医学のあゆみ. 142. 773-774 (1987)

小川泰弘：医学史。142. 773-774 (1987)

瀬口春道: 医学のあゆみ. 138. 232-233

Harumichi Seguchi：医学史。138. 232-233