Immunocytochemical study of the plasma membrane and the Cytoskeleton of the transitional epithelium.

我们采用免疫组织化学和快速冷冻深蚀刻复制技术研究了移行上皮细胞细胞骨架的超微结构和生化特征，以及管腔质膜和细胞骨架之间的关系。肌动蛋白丝，与抗肌动蛋白抗体和重酶解肌球蛋白反应，存在于腔质膜之下。这些细丝附着在管腔质膜上，形成网状结构。它们也附着在梭形囊泡和线粒体上。与抗角蛋白抗体反应的角蛋白丝在移行上皮细胞的胞质中相互平行排列成束。它们从不直接附着在管腔质膜上。在移行上皮中未发现与抗波形蛋白或抗结蛋白抗体反应的纤维。为了了解腔质膜和丝在该上皮中的命运，我们研究了作为溶酶体标记酶的酸性磷酸酶（ACPasa）和三偏磷酸酶（TMP酶）活性的超微结构定位。大多数溶酶体呈ACEI阳性，少数为TMP酶阳性。为了阐明这两种溶酶体之间的关系，需要进一步的研究。

We have investigated the ultrastructural and biochemical characteristics of the cytoskeleton, and the relationship between the luminal plasma membrane and cytoskeleton in the transitional epithelial cells employed immunohistochemistry and rapid-freeze-deep-etching replicas. The actin filaments, reacted with anti-actin antibody and heavy meromyosin, existed just beneath the luminal plasma membrane. These filaments attached to the luminal plasma membrane and formed meshwork. They also attached to fusiform vesicles and mitochondria. Keratin filament which reacted with anti-keratin antibody ran parallel each other forming bundles in the cytoplasm of transitional epithelial ells. They never attached directly to the luminal plasma membrane. We never found the filaments which reacted with anti-vimentin or anti-desmin antibody in the transition epithelium. In order to know the fate of the luminal plasma membrane and the filaments in this epithelium, we have studied the ultrastructural localization of acid phosphatase(ACPasa) and trimetaphosphatase(TMPase) activities used as marker enzymes of lysosomes. Most of lysosmes were ACPase positive a few of them were TMPase positive. To clarify the relationship between these two kind of lysosomes further investigation are neccessary.