The regulation of PEP carboxylase gene expression by nitrogen in maize leaf tissue

氮对玉米叶片组织中PEP羧化酶基因表达的调控

• 批准号: 02454010
• 负责人: SUGIYAMA Tatsuo
• 金额: $ 3.9万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454010/
• 关键词: Maize (Zea mays L.); PEP carboxylase; Carbonic anhydrase; Glutamin synthetase; Glutamate synthase; Regulation of Gene expression by nitrogen; C4-photosynthesis genes; cytokinin; トウモロコシ(<Zea>___ー <mays>___ー L.); 遺伝子発現の窒素による制御; Fdーグルタミン酸合成酵素; シコク

The following results were obtained by analyzing metabolic intermediates, enzyme activities, proteins, and mRNAs in the basal region of leaves, namely, photosynthetically maturing cells, of maize plants during recovery from N-stress.(A)The selective accumulation of PEP carboxylase(PEPC)is regulated primarily at transcriptional level in responding to N-availability. The N-avaialbility for induction of the N-dependent gene expression of PEPC was studied. The results obtained indicate that glutamine can be a positive signal for the control of the N-dependent gene expression of PEPC. The results also suggested that the accumulation of glutamine is mainly due to the differential expression of glutamine synthetase and ferredoxin-dependent-glutamate synthase during recovery from N-stress.(B)In addition to N-source cytokinin is an essential component for the induction of PEPC gene in the detached leaves of N-stressed plants, showing a hormonal specificity.(C)The gene expression of carbonic anhydrase(CA), a core enzyme in the C4pathway of photosynthesis, was analyzed in comparison to that of PEPC during recovery from N-stress. The results obtained show that CA and PEPC are coordinately regulated by the same or very similar mechanism in responding to N-availability.(D)The gene expression of glutamine synthetase located in the cytosols of mesophyil and bundle sheath cells was examined under various N-conditions. However, the levels of MRNA encoding for this enzyme unchanged regardless of N condition. This indicates that the N-dependent regulation of gene expression is specific at least for PEPC and CA genes.

通过对氮素胁迫恢复过程中玉米叶片基底部(即光合作用成熟细胞)的代谢中间产物、酶活性、蛋白质和mRNAs的分析，得到以下结果：(A)玉米叶片中PEP羧基酶(PEPC)的选择性积累主要在转录水平上调节，以响应N的有效性。研究了N-诱导PEPC N依赖基因表达的可行性。这些结果表明，谷氨酰胺可能是调控PEPC N依赖基因表达的一个积极信号。结果还表明，谷氨酰胺的积累主要是由于N胁迫恢复过程中谷氨酰胺合成酶和铁氧还蛋白依赖的谷氨酸合成酶的差异表达所致。(B)除了N源外，细胞分裂素是诱导N胁迫植物离体叶中PEPC基因的必需成分，具有激素特异性。(C)分析了光合作用C4途径的核心酶碳酸酐酶(CA)的基因表达，并与N胁迫恢复过程中PEPC的基因表达进行了比较。结果表明，CA和PEPC在对N有效性的响应中受到相同或非常相似的机制的协调调控。(D)检测了不同N条件下叶肉细胞和束鞘细胞胞浆中谷氨酰胺合成酶的基因表达。然而，无论氮素条件如何，该酶的编码水平都没有变化。这表明，至少对PEPC和CA基因而言，N依赖的基因表达调控是特异的。

项目成果

Bambang Sugiharto et al.: "Effects of nitrate and ammonium on gene expression of phosphoenolpyruvate carboxylase and nitrogen metabolism in maize leaf tissue during recovery from nitrogen stress." Plant Physiol.

Bambang Sugiharto 等人：“在氮胁迫恢复过程中，硝酸盐和铵对玉米叶组织中磷酸烯醇丙酮酸羧化酶基因表达和氮代谢的影响。”

James N.Burnell: "Light induction and the effect of nitrogen status upon the activity of carbonic anhybrase in maize leaves." Plant Physiology. 94. 384-387 (1990)

James N.Burnell：“光诱导和氮状态对玉米叶中碳酸酐酶活性的影响。”

SUGIHARTO Bambang: "Regulation of expression of carbonーassimilating enzymes by nitrogen in maize leaf" Plant Physiology. 92. 963-969 (1990)

SUGIHARTO Bambang：“玉米叶中氮对碳同化酶表达的调节”植物生理学 92. 963-969 (1990)。

Yuriko Kano-Murakami et al.: "Sequence-specific interactions of a maize factor with a GC-rich repeat in the phosphoenolpyruvate carboxylase gene." Mol. Gen. Genet.225. 203-208 (1991)

Yuriko Kano-Murakami 等人：“玉米因子与磷酸烯醇丙酮酸羧化酶基因中富含 GC 的重复序列的序列特异性相互作用。”

Mitsutaka Taniguchi et al.: "Functional differentiation and environmental adaptation of C4 photosynthetic cells." Plant Cell Technology. 4. 5-13 (1992)

Mitsutaka Taniguchi 等人：“C4 光合细胞的功能分化和环境适应。”