The Regulation of Photosynthesis and Nitrogen Assimilation Genes by Nitrogen in C4 Plants

氮对C4植物光合作用和氮同化基因的调控

• 批准号: 04454072
• 负责人: SUGIYAMA Tatsuo
• 金额: $ 3.84万
• 依托单位: NAGOYA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454072/
• 关键词: Maize (Zea mays); Panicum miliaceum; phosphoenolphruvate carboxylase; Carbon assimilation; Nitrogen assimilation; Regulation of gene expression by nitrogen; C4-photosynthesis; Cytokinin; 窒素ストレス; C_4光合成; ホスホエノールピルビン酸カルボキシラーゼ; カルボニックアンヒドラーゼ; トウモ

(1)The nitrate-inducible transcription of genes for nitrate reductase (NR), plastidic glutamine synthetase (GS2), and ferredoxin-glutamate synthase (Fd-GOGAT) is transient and precedes the gene transcription for phosphoenolpyruvate carboxylase (C4-PEPC) in maize mesophyll cells.(2)The nitrogen-inducible accumulation of mRNAs for PEPC and carbonic anhydrase (CA) requires cytokinin and glutamine as positive signals ; cytokinin up-regulates their transcription and glutamine up-regulates the accumulation of their mRNAs.(3)The cytokinin-dependent transcription of C4-PEPC requires the synthesis of protein(s) and the accumulation of C4-PEPC mRNA appears to be mediated by protein kinase and protein phosphatase activities, whereas the accumulation of mRNAs for NR and GS2 does not require the synthesis of protein.(4)The nitrogen-inducible accumulation of mRNAs for NR, GS2, and Fd-GOGAT does not requite cytokinin and glutamine acts as a negative signal for the accumulation of NR mRNA.It was suggested that protein phosphatase activity involves in the nitrate-dependent accumulation of mRNAs for NR and GS2.(5)Aspartate aminotransferase genes in Panicun miliaceum, an NAD-ME type of C4-plant, were cloned and sequenced. The gene expression of the isozyme which is located in the mesophyll cells was found to be selectively regulatee by nitrogen-availability.

（1）硝酸盐诱导的硝酸还原酶（NR）、质体谷氨酰胺合成酶（GS 2）和铁氧还蛋白-谷氨酸合酶（Fd-GOGAT）基因的转录是瞬时的，并且先于磷酸烯醇式丙酮酸羧化酶（C4-PEPC）基因的转录。（2）氮诱导PEPC和碳酸酐酶（CA）mRNA的积累需要细胞分裂素和谷氨酰胺作为正信号;细胞分裂素上调其转录，谷氨酰胺上调其mRNA的积累。（3）C4-PEPC依赖细胞分裂素的转录需要蛋白质的合成，C4-PEPC mRNA的积累似乎是由蛋白激酶和蛋白磷酸酶活性介导的，而NR和GS 2 mRNA的积累不需要蛋白质的合成。（4）NR、GS 2和Fd-GOGAT mRNA的积累不依赖于细胞分裂素，谷氨酰胺对NR mRNA的积累起负信号作用，提示蛋白磷酸酶活性参与了NR和GS 2 mRNA的积累。（5）克隆了NAD-ME型C4植物黍（Panicun miliaceum）的天冬氨酸氨基转移酶基因。该同工酶位于叶肉细胞中，其基因表达受氮素有效性的选择性调节。

项目成果

Son,D.,Jo,J.,and Sugiyama,T.: "Molecular cloning of an alanin aminotrasnferase from NAD-malic enzyme type-C4 plant Panicum miliaceum." Plant Mol.Biol.20. 705-713 (1992)

Son,D.、Jo,J. 和 Sugiyama,T.：“从 NAD-苹果酸酶 C4 型植物黍中分子克隆丙氨酸氨基转移酶。”

Bambang SUGIHARTO: "Effects of nitrate and ammonium on gene expression of PEP carboxylase and nitrogen metabolism in maize leaf tissue during recovery from nitrogen stress" Plant Physiol.98. 1403-1408 (1992)

Bambang SUGIHARTO：“氮胁迫恢复过程中硝酸盐和铵对玉米叶组织中 PEP 羧化酶基因表达和氮代谢的影响”Plant Physiol.98。

Sakakibara,H.,Kawabata,S.,Hase,T.,and Sugiyama,T.: "Differential effects of nitrate and light on the expression of glutamine synthetases and ferredoxin-dependent glutamate synthase in maize." Plant Cell Physiol.33. 1193-1198 (1992)

Sakakibara,H.、Kawabata,S.、Hase,T. 和 Sugiyama,T.：“硝酸盐和光对玉米中谷氨酰胺合成酶和铁氧还蛋白依赖性谷氨酸合成酶表达的不同影响。”

Taniguchi, M.and Sugiyama, T.: "Cloning and sequence analysis of cDNA encoding aspartate aminotransferase isozymes from Panicum miliaceum L., a C4 plant." Eur.J.Biochem.204. 611-620 (1992)

Taniguchi, M. 和 Sugiyama, T.：“来自 C4 植物 Panicum miliaceum L. 的编码天冬氨酸转氨酶同工酶的 cDNA 的克隆和序列分析。”

Taniguchi,M.and Sugiyama,T.: "Cloning and sequence analysis of cDNA encoding aspartate aminotransferase isozymes from Panicum miliaceum L.,a C4 plant." Eur.J.Biochem.204. 611-620 (1992)

Taniguchi, M. 和 Sugiyama, T.：“编码来自 C4 植物黍的天冬氨酸转氨酶同工酶的 cDNA 的克隆和序列分析。”