Molecular Basis for Organization of Photosynthetic Functions through Plant Body

植物体光合功能组织的分子基础

• 批准号: 09274102
• 负责人: SUGIYAMA Tatsuo
• 金额: $ 120.96万
• 依托单位: NAGOYA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas (A)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09274102/
• 关键词: Plant; Photosynthesis; Plant hormone; Cytokinin; Signal transduction; His-Asp phosphorelay system; Phloem translocation; Sulfate assimilation; His-Aspリン酸リレー情報伝達系; サイトカイニン合成酵素; サイトカイニン情報伝達系; 師管輸送タンパク質; グルタミン合成酵素; NADH-グルタミン酸合成酵素; 硫酸イオントランスポータ

1. Cytokinin was shown to act as the organ-to-organ signal, transmitted from the root to shoot, for the presence of nitrate in the soil. The sensor kinase responsive to cytokinin was indentified. Other components of the His-Asp phosphorelay system (i. e. response regulators and His-containing phophotransmitters), implicated in transduction of the cytokinin signal, were also identified and characterized. In addition, pseudo-response regulators, implicated in generation of circadian rhythm in plants, were identified and characterized.2. The genes encoding adenylate isopentenyltransferase, a cytokinin biosynthesis enzyme, were identified in Arabidopsis thaliana, demonstrating that the plant hormone is synthesized by the plant and not by symbiotic microorganisms.3. Phloem sap of rice was shown to contain certain proteins, including thioredoxin h, and several mRNA species. It was further shown that at least some of these macromolecules are synthesized in the companion cell and transferred to the sieve element via plasmodesmata.4. A particular set of glutamine synthetase and glutamate synthease isozymes, which are localized in the vascular bundle of leaf blade and maturing grains, respectively, was found to play an important role in mobilization of leaf nitrogen and its transfer to rice grains.5. Genes encoding sulfate transporters and sulfate assimilating enzymes were identified in A. thaliana and other plants. Regulatory links between the sulfur- and nitrogen-assimilating pathways were found.

1. 细胞分裂素作为器官到器官的信号，从根传递到茎，表明土壤中硝酸盐的存在。鉴定了对细胞分裂素有反应的传感器激酶。His-Asp磷传递系统的其他组成部分（即反应调节因子和含his的光递质），涉及细胞分裂素信号的转导，也被鉴定和表征。此外，还鉴定并鉴定了与植物昼夜节律产生有关的伪反应调节因子。腺苷酸异戊烯基转移酶是细胞分裂素的一种生物合成酶，在拟南芥中发现了编码腺苷酸异戊烯基转移酶的基因，表明这种植物激素是由植物自身合成的，而不是由共生微生物合成的。水稻韧皮部汁液含有某些蛋白质，包括硫氧还蛋白h和几种mRNA。结果进一步表明，这些大分子中至少有一部分是在伴生细胞中合成的，并通过胞间连丝转移到筛元上。谷氨酰胺合成酶和谷氨酸合成酶同工酶分别位于叶片维管束和成熟籽粒中，它们在水稻叶片氮素的动员和向籽粒的转运中起着重要作用。硫酸盐转运蛋白和硫酸盐同化酶的编码基因在拟南芥和其他植物中被鉴定出来。发现了硫和氮同化途径之间的调节联系。

项目成果

Ohba, H., Sano, H.et al.: "Diverse response of rice and maize genes encoding homologs of WPK4,an SNF1-related protein kinase from wheat,to light,nutrients,low temperature and cytokinins"Mol.Gen.Genet.. 263. 359-366 (2000)

Ohba, H.、Sano, H.等人：“编码 WPK4（一种来自小麦的 SNF1 相关蛋白激酶）同源物的水稻和玉米基因对光、营养物、低温和细胞分裂素的不同反应”Mol.Gen.Genet

Nozawa, A., Sano, H.et al.: "An Arabidopsis SNF1-related protein kinase,AtSR1,interacts with a calcium-binding protein,AtCBL2,of which transcripts respond to light"Plant Cell Physiol.. 42. 976-981 (2001)

Nozawa, A., Sano, H.等人：“拟南芥 SNF1 相关蛋白激酶 AtSR1 与钙结合蛋白 AtCBL2 相互作用，其转录物对光有反应”植物细胞生理学.. 42. 976-

Takahashi, H., Saito, K.et al.: "Cloning of an Arabidopsis cDNA encoding a chloroplast localizing sulfate transporter isoform"J.Exp.Bot.. 50. 1713-1714 (1999)

Takahashi, H., Saito, K.等：“编码叶绿体定位硫酸盐转运蛋白亚型的拟南芥 cDNA 的克隆”J.Exp.Bot.. 50. 1713-1714 (1999)

Goto, S., Yamaya, T.et al.: "Organization and structure of NADH-dependent glutamate synthase gene in rice plants"Biochim.Biophys.Acta. 1387. 298-308 (1998)

Goto，S.，Yamaya，T.等人：“水稻植物中NADH依赖性谷氨酸合酶基因的组织和结构”Biochim.Biophys.Acta。

Hirose, N., Yamaya, T.: "Okadaic acid mimics nitrogen-stimulated transcription of NADH-glutamate synthase gene in rice cell cultures"Plant Physiol.. 121. 805-812 (1999)

Hirose, N., Yamaya, T.：“冈田酸在水稻细胞培养物中模拟 NADH-谷氨酸合酶基因的氮刺激转录”植物生理学.. 121. 805-812 (1999)