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Regulatory Mechanism by Nitrogen of Gene Expression of Enzymes Involved in Photosynthesis and Nitrogen-Assimilation Pathways

氮对光合作用和氮同化途径相关酶基因表达的调控机制

基本信息

批准号：
61480056
负责人：
SUGIYAMA Tatsuo
金额：
$ 4.16万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480056/
关键词：
Maize RubisCO PEP carboxylase pyruvate-Pi dikinase glutamine synthetase,GOGAT mRNA,carbon assimilating enzyme nitrogen assimilating enzyme glutamate synthase Pi dikinase pyruvate 炭酸同化系酵素窒素同化系酵素

项目摘要

The results obtained in this project were summarized as follows.1. The regulation of gene expression by nitrogen of carbon assimilating enzymes in maize leaf was studied by examining partitioning of nitrogen into rubisCO, PEP carboxylase, and pyruvate, Pi dikinase in transitional state during supplementing nitrogen to nitrogen deficient plant seedlings grown under near optimal light and temperature conditions. In a fully developed youngest leaf a differential accumulation of protein and mRNA was observed among these three enzymes. Based upon the data PEP carboxylase was judged as a most limiting enzyme to photosynthesis in maize leaf. An evidence was also provided to suggest that nitrogen is a regulatory factor essential for the light-stimulation of accumulation of mRNA and protein for these three enzymes.2. Aspartate aminotransferase was purified from the mesophyll cells of Panicum maximum trichoglume , a PEP carboxykinase type of C_4 plant. Partial characterization of the enzyme which appears to involve photosynthetic carbon assimilation was achieved and its specific polyclonal antibody was prepared. Using this antibody the complementary cDNA of this enzyme was prepared from DNA library of mRNAs derived from Eleusine coracana, a NAD- malic enzyme type of C_4 plant. Also, antibodies against Gln synthetase and GOGAT were prepared.3. Light/dark regulation of maize leaf PEP carboxylase was studies and its possible mechanism was proposed based upon analysis of dark-inactivated and light-activated forms.

本项目取得的主要成果如下：1.以玉米缺氮幼苗为材料，研究了在近适光温条件下，氮素对玉米叶片中RubisCO、PEP羧化酶以及过渡态丙酮酸、Pi二激酶的分配，探讨了氮素对玉米叶片碳同化酶基因表达的调控。在一个完全发达的最年轻的叶片中，观察到这三种酶之间的蛋白质和mRNA的差异积累。据此，认为PEP羧化酶是玉米叶片光合作用的最大限制酶。同时也证明了氮是光刺激这三种酶的mRNA和蛋白质积累的重要调控因子.从C_4植物大穗黍（Panicummaximalglume）的叶肉细胞中分离纯化了一种PEP羧激酶型天冬氨酸氨基转移酶。对该酶进行了部分表征，并制备了其特异性多克隆抗体。用此抗体从C_4植物牛筋草（Eleusinecoracana）的cDNA文库中扩增出该酶的互补cDNA。同时制备了抗谷氨酰胺合成酶和GOGAT的抗体.研究了玉米叶片PEP羧化酶的光/暗调控，并通过对暗失活和光激活形式的分析，提出了其可能的调控机制。

项目成果

期刊论文数量（36）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Steven C. Huber;Tatsuo Sugiyama: Plant Physiol.81. 674-677 (1986)

Steven C. Huber；Tatsuo Sugiyama：植物生理学.81。

DOI：
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通讯作者：

Steven C. Huber, Tatso Sugiyama, and Takashi Akazawa: "Light modulation of maize leaf phosphoenolpyruvate carboxylase" Plant Physiol.82. 550-554 (1986)

Steven C. Huber、Tatso Sugiyama 和 Takashi Akazawa：“玉米叶磷酸烯醇丙酮酸羧化酶的光调节”Plant Physiol.82。

DOI：
发表时间：
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影响因子：
0
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通讯作者：

Katsura Izui, Sumio Ishijima, Yasunori Yamaguchi, Fumiaki Katagiri, Takuya Murata, Katuya Shigesada, Tatsuo Sugiyama, and Hirohiko Katsuki: "Cloning and sequence analysis of cDNA encoding active phosphoenolpyruvate carboxylase of the C_4-pathway from maiz

Katsura Izui、Sumio Ishijima、Yasunori Yamaguchi、Fumiaki Katagiri、Takuya Murata、Katuya Shigesada、Tatsuo Sugiyama 和 Hirohiko Katsuki：“编码玉米 C_4 途径活性磷酸烯醇丙酮酸羧化酶的 cDNA 的克隆和序列分析