权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Studies on post-mortem biochemical changes in the muscle of marine invertebrates

海洋无脊椎动物死后肌肉生化变化的研究

基本信息

批准号：
02454082
负责人：
YAMANAKA Hideaki
金额：
$ 3.71万
依托单位：
Tokyo University of Fisheries
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

1. During storage at low tempeatures ATP in the muscle of kuruma prawn decreased remarkably and AMP and IMP were accumulated. K values increased slowly. Contents of glycogen and glycolytic intermediates were relatively low and metabolized smoothly to lactate. Major free amino acids were glycine, arginine, proline, glutamic acid, alanine and taurine. Ornithine increased during storage and was useful as an index for freshness. Only putrescine was detected as a polyamine Lipid content was about 1%, and phospholipid and sterol were main components. Phospholipid was mainly composed of phosphatidylethanolamine and phosphatidylcholine. During storage EPA content decreased and was the main unsaturated fatty acid which was autoxidized.2. Degradation of ATP, decrease of arginine phosphate and increase of octopine in the adductor muscle of scallop occurred most rapidly during storage at -3ﾟC, followed by 0ﾟC and slowest at 5ﾟC Large amounts of AMP was not accumulated and inosine together with hypoxanthine was produced at the early stage of storage. Small amounts of IMP and adenosine were detected, and AMP was decomposcd to Inoslne through two pathways. D-lactate increased and accumulated during storage and was an end-product of glycolysis, together with octopine. D-lactate appeared to be useful as an index for freshness.3. During storage of ascidian muscle ATP, ADP and AMP decreased, while inosine and hypoxanthine increased. Hypoxanthine was useful as an index for freshness. IMP and adenosine were detected in small amounts and AMP was degraded to inosine through two pathways. Increase of K value was fairly rapid. Glycogen content was lowest(2-3%) in February and highest(8-9%) in august. Glucose increased remarkably during storage and formed through amylolytic pathway from glycogen Glucose 6-phosphate, fructose 6-phosphate, and fructose 1,6-diphosphate were detected in fairly high amounts. During storage lactate was produced and accumulated.

1.在低温贮藏过程中，库鲁玛对虾肌肉中的ATP含量显著下降，并积累了AMP和IMP。K值增长缓慢。糖原和糖酵解中间产物的含量相对较低，并顺利代谢为乳酸。主要游离氨基酸为甘氨酸、精氨酸、脯氨酸、谷氨酸、丙氨酸和牛磺酸。鸟氨酸在贮藏过程中有所增加，可作为新鲜度的指标。只有腐胺以多胺形式存在，脂肪含量约为1%，主要成分为磷脂和甾醇。磷脂主要由磷脂酰乙醇胺和磷脂酰胆碱组成。在贮藏过程中，EPA含量下降，是自氧化的主要不饱和脂肪酸。在-3ﾟC贮藏过程中，扇贝内收肌中三磷酸腺苷降解最快，磷酸精氨酸减少，章鱼碱升高最快，0ﾟC次之，5ﾟC贮藏最慢。在贮藏初期，大量的腺苷没有积累，并与次黄嘌呤一起产生。检测到少量的IMP和腺苷，AMP通过两条途径分解为Inoslne。D-乳酸在储存过程中增加和积累，是糖酵解的最终产物，与章鱼碱一起。D-乳酸似乎是一个有用的新鲜度指标。海鞘肌肉在贮藏过程中，ADP和AMP含量下降，肌苷和次黄嘌呤含量升高。次黄嘌呤可作为新鲜度的指标。IMP和腺苷含量较低，AMP通过两条途径降解为肌苷。K值上升较快。糖原含量在2月份最低(2-3%)，8月份最高(8-9%)。葡萄糖在贮藏过程中显著增加，并通过糖原淀粉分解途径形成，其中葡萄糖6-磷酸、6-磷酸果糖和1，6-二磷酸果糖含量较高。在贮藏过程中，会产生和积累乳酸。