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Clinico-Pathological Studies on Canine Babesiosis

犬巴贝斯虫病的临床病理学研究

基本信息

批准号：
02454108
负责人：
ONO Kenichiro
金额：
$ 4.48万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454108/
关键词：
Babesiosis dog mouse B. rodhaini B. microti immune response isoenzyme splenic cell subpopulation 宿主感染応答抗原特異性糖代謝機構

项目摘要

Clinico-pathological. analysis on Babesia Microti and Babesiarodhaini infection in mice were performed.1) Immunological responses of splenic cell from mice inoculated with B. rodhaini were examined. Splenic cells form mice-inoculated wlth B. rodhaini were significantly proliferated against homogenous Babesia soluble lysate antigens and also enhanced Interleukin 2 production. The major proliferative cells were seemed to be T cell, especially helper T cell, because of the proliferative responses were nearly completely abolished by pre-treatments of splenic cells with anti-Thy-1 and Anti Lyt-1.2 antibodies. Splenic cells from immunized mice showed protective activity against B. rodhaini inoculation, whereas splenic"cells from hyperimmunized mice showed. no activity. However, pretreated With anti-T cell, ant-Lyt-1.2, and anti Lyt-2.2 antibodies, splenic cells revealed protective activities against B. rodhaini inoculation. Changes of splenic lymphocyte subpopulation after B. microti and B. rodhaini were examined. The Thy-1 positive cell number in B. microti inoculated mice were significantly higher than that in B. rodhaini inoculated mice. The ratio in L3T4/Lyt-2 positive cell after inoculation with-B. rodhaini inoculated mice revealed a lack of an increasing phase. These results suggested that the T cell dependent early immune response, especially suppressor activity, was closely related to the difference in the course of infection between the non-lethal B. microti and the lethal B. rodhaini infection in mice.2) Isoenzyme patters of glucose-6-phosphate -dehydrogenase (G6PD), malate dehydrogenase (MDH), and lactate dehydrogenase (LDH) were examined. There were no difference in G6PD and MDH between 2 strains, however, the quite difference in LDH isoenzyme pattern were observed between these 2 strains.

二。分析小鼠微巴贝斯虫和巴贝斯虫感染情况。1)观察鼠脾细胞免疫应答。小鼠脾细胞对同种巴贝斯虫可溶性裂解物抗原有明显增殖，白细胞介素2的产生也有明显增加。脾细胞经抗thy -1和抗Lyt-1.2抗体预处理后，增殖反应几乎被完全消除，主要增殖细胞为T细胞，尤其是辅助性T细胞。免疫小鼠脾细胞对罗氏弧菌接种具有保护作用，而过度免疫小鼠脾细胞对罗氏弧菌接种具有保护作用。没有活动。然而，经抗t细胞、抗lyt -1.2和抗Lyt-2.2抗体预处理后，脾细胞显示出对罗德海螺旋体接种的保护作用。观察微螺旋体和罗德海螺旋体感染后脾淋巴细胞亚群的变化。微螺旋体接种小鼠的Thy-1阳性细胞数显著高于罗德海螺旋体接种小鼠。接种- b后L3T4/Lyt-2阳性细胞的比例。Rodhaini接种的小鼠显示缺乏生长阶段。这些结果表明，T细胞依赖性的早期免疫反应，特别是抑制活性，与小鼠非致死性微小贝氏菌和致死性罗德海贝氏菌感染过程的差异密切相关。2)检测葡萄糖-6-磷酸脱氢酶（G6PD）、苹果酸脱氢酶（MDH）和乳酸脱氢酶（LDH）同工酶谱。G6PD和MDH在两株间无显著差异，而LDH同工酶谱在两株间差异较大。