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Glucose metabolism and Molecular basis analysis for immunoprotective mechanisms angainst Babesia spp. Infection

巴贝虫属免疫保护机制的葡萄糖代谢和分子基础分析。

基本信息

批准号：
12556055
负责人：
ONO Kenichiro
金额：
$ 8.32万
依托单位：
THE UNIVERSITY OF TOKYO
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12556055/
关键词：
Babesia spp glucose metabolism membrane specific protein protective immunity IL-12 IL-12 p70 stimulating factor for IL-12 production IRBM Il-12産生刺激物質

项目摘要

1) Glucose metabolism : Using short term cultivation system established, glucose uptake system was investigated in Babesia microti and B. rodhaini infected erythrocytes. Based on the labeled D-glucose uptake, both infected erythrocytes showed new glucose transport system, which revealed different character between two Babesia protozoa. On the morphological examination, specific changes observed in both infected erythrocytes.2) Development of anti-protozoan drugs : Based on the results of glucose metabolism related enzyme activities, some candidate designs of anti-protozoan drugs was imaged. However, no drug was developed.3) Molecular analysis for protozoan membrane proteins : Based on the results of molecular analysis for surface membrane proteins of infected erythrocytes, one protein enhanced IL-12 production from splenic macrophage was detected. However, full sequence of its was not established.4) Immunological protective mechanism for protozoa : Differentiation for Th1 cells was an important factor for prevention of protozoan infection. Two phase secretion of IL-12 p70 was closely related to this differentiation. However, other mechanism beside IL-12 p70 was necessary for the elimination of protozoa.5) Development of the biological response modifier : The heat stable protein in culture supernatant of infected erythrocytes was detected. Since this protein remarkably enhanced IL-12 production from splenic macrophage, iit was considered that one of the candidate for biological response modifier.

1)糖代谢：利用建立的短期培养体系，研究微小巴贝斯虫和罗氏巴贝斯虫感染红细胞的葡萄糖摄取系统。根据标记的D-葡萄糖摄取，两个感染的红细胞都显示了新的葡萄糖转运系统，这显示了两种巴贝斯虫原虫之间的不同特征。2)抗原虫药物的研究进展：根据糖代谢相关酶活性的结果，设计了一些抗原虫药物的候选方案。3)原虫膜蛋白的分子分析：根据对感染红细胞表面膜蛋白的分子分析结果，检测到一种促进脾巨噬细胞产生IL-12的蛋白。4)原虫免疫保护机制：Th1细胞分化是预防原虫感染的重要因素。IL-12p70的双时相分泌与这种分化密切相关。5)生物反应调节剂的研制：检测到感染红细胞培养上清液中的热稳定蛋白。由于该蛋白显著促进脾巨噬细胞产生IL-12，IIT被认为是生物反应调节剂之一。