Analysis of immature B cell activation and differentiation

未成熟 B 细胞活化和分化分析

• 批准号: 07457088
• 负责人: SAKAGUCHI Nobuo
• 金额: $ 4.8万
• 依托单位: KUMAMOTO UNIVERSITY (1996-1997)Tottori University (1995)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457088/
• 关键词: pre-B cell; rapamycin; phosphatase; MB-1; signal transduction; 増殖; 免疫抑制剤; PP2A; 抗原レセプター; Igα(MB-1); α4; ホスファターゼ; 細胞周期; 細胞増殖; pre-B細胞; 細胞分化; 胎児

To study the activation and development of immature B lineage cells, we searched the unique signal transduction molecules expressed in the immature B cells or in the process of B cell activation.We characterized the pre-BCR signal transduction molecules, among which PI-3 kinase activity was shown to be involved in the pre-B cell stages. We also isolated a molecule named immunoglobulin-receptor binding protein (IGBP-1/alpha4) , which is originally identified as a phosphoprotein co-precipitated with the BCR-component MB-1 molecule. The alpha4 gene was isolated and characterized. The recent results also showed that the alpha4 protein is associated with the protein phosphatase 2 A,whose association is critical for the proliferation of B cells. The alpha4/PP2A complex is shown to be a novel target of rapamycin. These results implicated the novel signal transduction pathway involved in the proliferation of lymphoid cells.

为了研究未成熟B细胞的活化和发育过程，我们寻找了未成熟B细胞或B细胞活化过程中表达的独特信号转导分子，并对前BCR信号转导分子进行了表征，其中PI-3激酶活性参与了前B细胞阶段。我们还分离出一种名为免疫球蛋白受体结合蛋白（IGBP-1/α 4）的分子，最初被鉴定为与BCR组分MB-1分子共沉淀的磷蛋白。α 4基因的分离和表征。最近的结果还表明，α 4蛋白与蛋白磷酸酶2 A相关，其相关性对于B细胞的增殖至关重要。α 4/PP 2A复合物被证明是雷帕霉素的新靶点。这些结果暗示了参与淋巴细胞增殖的新的信号转导途径。

项目成果

Igarashi H.: "Telomerase activity is induced in human peripheral B lymphocytes by the stimulation to antigen receptor." Blood. 89. 1229-1307 (1997)

Igarashi H.：“通过刺激抗原受体，在人外周 B 淋巴细胞中诱导端粒酶活性。”

Igarashi H.: "Telomerase activity is induced by the stimulation antigen receptor in human periphera lymphocytes." Biochem.Biophys.Res.Commun.219. 649-655 (1996)

Igarashi H.：“端粒酶活性是由人外周淋巴细胞中的刺激抗原受体诱导的。”

Yoshinobu Matsuo, Roderick AF MacLeod, Hans G.Drexler, Kensuke Kojima, Kazuhiko Kuwahara, Chiharu Nishizaki, Shunichi Fukuda, Yuko Inoue, Tatsuo Sezaki, Nobuo Sakaguchi, and Kunzo Orita.: "A novel ALL-L3 cell line, BALM-16, lacking expression of immunoglo

Yoshinobu Matsuo、Roderick AF MacLeod、Hans G.Drexler、Kensuke Kojima、Kazuhiko Kuwahara、Chiharu Nishizaki、Shunichi Fukuda、Yuko Inoue、Tatsuo Sezaki、Nobuo Sakaguchi 和 Kunzo Orita。：“一种新型 ALL-L3 细胞系，BALM-16

Matsuo Y.: "Four subclones with distinct immunoglobulin light chain phenotypes(χ^+λ^+,χ^+,λ^+and χ^-λ^-)from acute leukemia." Leukemia. 10. 700-706 (1996)

Matsuo Y.：“来自急性白血病的具有不同免疫球蛋白轻链表型（χ^+λ^+、χ^+、λ^+ 和 χ^-λ^-）的四个亚克隆。” ）

Masashi Baba, Yuji Kikuchi, Shigeo Mori, Hiroshi Kimoto, Seiji Inui, Nobuo Sakaguchi, Jun-ichiro Inoue, Tadashi Yamamoto, Toshitada Takemori, Maureen Howard and Kiyoshi Takatsu.: "Mouse germinal center B cells with the xid mutation retain responsiveness t

Masashi Baba、Yuji Kikuchi、Shigeo Mori、Hiroshi Kimoto、Seiji Inui、Nobuo Sakaguchi、Jun-ichiro Inoue、Tadashi Yamamoto、Toshitada Takemori、Maureen Howard 和 Kiyoshi Takatsu。：“具有 xid 突变的小鼠生发中心 B 细胞保留了响应性