Clinical usefulness of Gd-DTPA in MRI and its effect upon lymphocyte chromosomes.

Gd-DTPA 在 MRI 中的临床用途及其对淋巴细胞染色体的影响。

• 批准号: 02454283
• 负责人: SUZUKI Soji
• 金额: $ 2.82万
• 依托单位: Tokyo Medical & Dental University (TMDU)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454283/
• 关键词: MRI; Magnetic field; Gd-DTPA (the contrast medium in MRI; SCE; GdーDTPA; 染色分体交換に対する影響

CLINICAL RESULT :Signal intensities in various imaging techniques in MRI were calculated and the most adequate selection of TR and TE in each technique was postulated. Based upon the postulation, a combination of MR angiography(the maximum intensity projection, 3-D FT)and an administration of GD-DTPA was tried in cases of brain tumor with success.EXPERIMENTAL RESULT :The effects of a magnetic field, radiofrequency, and GD-DTPA on the sister chromatid, , exchange(SCE)in peripheral lymphocytes were investigated. Two ml of whole blood in tubes was exposed to magnetic force(1.5 T)and/or radiofrequency waves(63. -86 MHz, 0.4 W/kg of SAR). GD-DTPA was added at 1.25, 12.5 or 125 mol/ml per 2 ml of whole blood to some samples. Slides were made for SCE investigation, after the blodd was exposed to various conditions and cultured for 69 hours.The frequency of SCEs were counted and evaluated using Student's t test. As the concentration of GD-DTPA rose, the frequency of SCE increased. Magnetic force and radiofrequency also induced some SCE aberrations. However, at the level of concentration of GD-DTPA in the clinical use, the contrast medium in MRI did not effect SCE frequency.

临床结果：计算了MRI中各种成像技术的信号强度，并假设了每种成像技术中TR和TE的最适当选择。在此基础上，在脑肿瘤病例中尝试了MR血管造影（最大强度投影，3-D FT）和GD-DTPA的结合，并取得了成功。实验结果：研究了磁场、射频和GD-DTPA对外周血淋巴细胞姐妹染色单体交换（SCE）的影响。将2 ml的全血置于1.5 T的磁力和/或射频波（63 T）下。-86 MHz, 0.4 W/kg SAR)。在一些样品中以每2ml全血1.25、12.5或125 mol/ml加入GD-DTPA。将血液暴露于各种条件下培养69小时后，制作载玻片用于SCE调查。使用Student's t检验统计和评价sce的频率。随着GD-DTPA浓度的升高，SCE的发生频率增加。磁力和射频也会引起SCE畸变。然而，在临床使用的GD-DTPA浓度水平上，MRI造影剂对SCE频率没有影响。

项目成果

鈴木 宗治: "MRIー最新の進歩" 臨床成人病. 21. 359-360 (1991)

Muneharu Suzuki：“MRI - 最新进展”临床成人疾病。21. 359-360 (1991)

Suzuki, Soji: "Basic aspects of MRI contrast media : (1), (2), (3) & (4)" Clinical Radioloy. 36(9), 36(10), 36(11), 36(12). 1075-1076, 1173-1174, 1599-1600, 1701-1702 (1991)

Suzuki, Soji：“MRI 造影剂的基本方面：(1)、(2)、(3)

山田 一郎: "脳神経系の造影MRI" Innervision. 6(11). 27-32 (1991)

Ichiro Yamada：“颅神经系统的对比 MRI”Innervision 6(11)。

山田 一郎: "Moyamoya Disease:Its Piagnosis by Threeーdimentional Timeーofーflight MRA ngiography" Radiolgy(U.S.A),accepted on Feb.5,1992.

Ichiro Yamada：“烟雾病：三维飞行时间 MRA 血管造影的诊断”放射学（美国），1992 年 2 月 5 日接受。

鈴木 宗治: "MRI用造影剤の基礎(1)(2)(3)(4)" 臨床放射線(夏). 36. (1991)

Muneharu Suzuki：“MRI 造影剂基础知识 (1)(2)(3)(4)”临床放射学（夏季）36。（1991 年）。