Histologic study of cellular response to graft material on hybrid-type vascular prosthesis.

小口径人造血管的长期通畅率仍不令人满意。为了获得更好的通畅性，研究了一种将活细胞与人工血管相结合的混合型人工血管。我们研究了内皮细胞在超细聚酯纤维移植物上的接种，并发展了过滤接种法，以便大量利用细胞。体内外实验结果显示，内皮细胞在移植物周围生长，并在移植后3个月保持单层连续。然而，在吻合口处，EC单层形成于厚的内皮下组织上，该组织延续到宿主动脉的厚内膜。单纯的内皮细胞接种可能不足以防止移植物的衰竭。内膜增生可能是后续研究中实现小口径人工血管良好通畅性的主要目标。

A small caliber vascular prosthesis remains unsatisfactory in regard with the long term patency rate. A hybrid vascular prosthesis has been investigated for achieving the better patency in the way of combining the living cells with a synthetic graft. We studied the endothelial cells (ECs) seeding to the ultrafine polyester fiber grafts and developed the filtration seeding method in order to utilize a large number of cells. The in vitro and in vivo study revealed that the ECs grew up around the grafts with time and maintain the continuous monolayer of the ECs three months after implantation. However, at the anastomotic sites, the ECs monolayer was formed on the thick subendothelial tissue which continued to the thick intima of the host artery. The ECs seeding only may not be enough to prevent the graft failure. The intimal hyperplasia may be the major target for achieving the better patency of the small caliber vascular prosthesis in the following research.