Histologic study of cellular response to graft material on hybrid-type vascular prosthesis.

混合型血管假体上细胞对移植材料反应的组织学研究。

• 批准号: 02454296
• 负责人: SATO Osamu
• 金额: $ 1.73万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454296/
• 关键词: endothelial cell seeding; prelined graft; hybrid-type vascular prosthesis; small caliber vascular prosthesis; endothelial cell; Endothelial Cell Seeding; Predined graft; Endothelial cell seeding; Prelined graft

A small caliber vascular prosthesis remains unsatisfactory in regard with the long term patency rate. A hybrid vascular prosthesis has been investigated for achieving the better patency in the way of combining the living cells with a synthetic graft. We studied the endothelial cells (ECs) seeding to the ultrafine polyester fiber grafts and developed the filtration seeding method in order to utilize a large number of cells. The in vitro and in vivo study revealed that the ECs grew up around the grafts with time and maintain the continuous monolayer of the ECs three months after implantation. However, at the anastomotic sites, the ECs monolayer was formed on the thick subendothelial tissue which continued to the thick intima of the host artery. The ECs seeding only may not be enough to prevent the graft failure. The intimal hyperplasia may be the major target for achieving the better patency of the small caliber vascular prosthesis in the following research.

对于长期通畅率，小口径的血管假体仍然不令人满意。已经研究了杂种血管假体，以实现将活细胞与合成移植物结合的更好的通畅性。我们研究了内皮细胞（ECS）播种到超铁纤维纤维移植物中，并开发了过滤播种方法，以利用大量细胞。体外和体内研究表明，EC随着时间的流逝而在移植物周围长大，并在植入后三个月保持ECS的连续单层。但是，在吻合部位，ECS单层是在厚的下皮组织上形成的，该组织持续到宿主动脉的厚内膜。 ECS播种可能不足以防止移植物衰竭。在以下研究中，内膜增生可能是实现小口径血管假体更高通畅的主要目标。

项目成果

白川 元昭他: "Filtration法による内皮細胞seedingグラフトの短期回収例における組織学的検討" 人工臓器. 19(3). 1297-1300 (1990)

Motoaki Shirakawa 等：“通过过滤法短期恢复内皮细胞移植物的组织学研究”Artificial Organs 19(3) 1297-1300 (1990)。

Motoaki,S,Shunya,S,Tetsuro,M,Jyun,E,Yutaka,T.,Osamu,S,Atsuhiko,T,Yusuke,T: "Animal experiment of the endothelial seeding graft by filtration method.(in Japanese)" Artificial Organ.19. 1297-1300 (1990)

Motoaki,S,Shunya,S,Tetsuro,M,Jyun,E,Yutaka,T.,Osamu,S,Atsuhiko,T,Yusuke,T：“过滤法内皮种植移植物的动物实验。（日语）”

Shuuya Shindo他: "Perianastomotic findings of the endothilial cell sealed grafts at three pronths" ASAIO Joiirnal.

Shuuya Shindo 等人：“三门端内皮细胞密封移植物的吻合口周围发现”ASAIO Joiirnal。

進藤 俊哉他: "イヌ自家静脈内皮細胞と超極細ポリエステル繊維によるハイブリッド小口径人工血管の移植実験" 人工臓器. 19(3). 1361-1364 (1990)

Toshiya Shindo 等：“使用犬自体静脉内皮细胞和超细聚酯纤维的混合小直径人造血管移植实验”Artificial Organs 19(3) (1990)。

Motoaki,S,Shunya,S,Tetsuro,M,Jyun,E,Yutaka,T,Osamu,S,Atsuhiko,T,Yusuke,T,Yasuo,I,Shyoji,N,Moto,K,: "Animal experiment of the endothelial Seeding graft by filtration method." Artifical Organ. 19. 1297-1300 (1990)

元明，S，俊哉，S，铁郎，M，Jyun，E，丰，T，修，S，敦彦，T，佑介，T，康夫，I，庄司，N，本，K，：“动物实验