Cerebrospinal fluid and blood circulation in subependymal tissue in hydrocephalus- Effect of cerebrospinal fluid diversion

结果：分流术前室管膜细胞正下方室管膜下组织含水量为72.2±L。0%，明显高于深部脑白质组织含水量的67.2±0.7%。结果表明，脑室表面正下方的含水量明显增加，但高于深部白质组织的67.2±0.7%。分流组室管膜下组织含水量为70.9±1.1%，与对照组无明显差异。在分流后不能发挥功能的动物中，水分含量并没有像预期的那样下降，有趣的是，其深层组织中的水分含量增加了。但电子显微镜检查未见脑室深部白质细胞外间隙明显扩大，轴突和髓鞘纤维排列紊乱也不明显。从我们的一系列实验中了解到，如果分流术不能缩小脑室大小，脑室深部白质中的水分含量虽然增加了，但距离室管膜表面只有2~3 mm，这表明室管膜下组织作为脑脊液循环的辅助途径发挥了重要作用。

Results :Before shunt was put in water content in the subependymal tissue immediate underneath the ependymal cells was 72.2+__-l. 0 % and much increased than the water content of deep white matter tissue of 67.2 +__- 0.7 % The results indicate that water content is definitely increased just underneath the ventricular surface but increased than the water content of deep white matter tissue of 67.2+__-0.7 %. In the animals in which shunt did work, its water content in the subependymal tissue was 70.9+__-1.1% and this was not different from the control animals. In animals which failed to function after shunting water content did not decreased as might be expected and interestingly enough its water content in the deeper tissue was increased. Electron microscopic examination, however, did not show marked enlargement of the extracellular space in the deep white matter and also derangement of the axons and myelin fibers were not prominent at all.From the series of our experiment it was learned that if shunt procedure failed to reduce the ventricular size water content in the deep white matter, when say that it is only 2 or 3 mm from the ependymal surface though increased definitely suggesting an important role of the subependymal tissue as a subsidiary way of cerebrospinal fluid circulation.