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Morphological studies for neurotransmission in the trigeminal nervous system -Roles of neuropeptides and amino acid-.

三叉神经系统神经传递的形态学研究-神经肽和氨基酸的作用-。

基本信息

批准号：
02454464
负责人：
UEDA Yutaka
金额：
$ 4.03万
依托单位：
Osaka Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

Changes in the levels of transcription factor mRNA and neuropeptide precursor mRNA in the trigeminal nucleus caudalis (TNC) in response to orofacial pain induced by the injection of formalin into the facial skin of anesthetized rats were investigated. After the injection of formalin, the number of neurons expressing c-fos, NGFI-A and NGFI-B mRNA was increased, followed by increases in the number of neurons expressing preprogalanin (PPG), preproenkephalin A (PPEA), preprodynorphin (PPD) and preprotachykinin (PPT) mRNA in the superficial layers (laminae I and II) of the ipsilateral TNC in accordance with the projection system previously described. The increases in the number and intensity of the labeled neurons for PPG, PPEA, PPD and PPT mRNA were most marked 6 hrs after the injection of formalin. The increases in the number and intensity of the labeled neurons for c-fos, NGFI-A and NGFI-B mRNA were most marked at 2 hrs.Furthermore, the numbers of PPD and PPT mRNA containing neurons were … More increased in the contralateral TNC after the injection. The number of Fos-like immunoreactivity positive neurons was also increased in the contralateral TNC.These findings suggested that the modulation of pain is associated with the increased production of PPG, PPE, PPD and PPT, and the transcription of these genes are regulated by immediate early genes, such as c-fos, NGFI-A and NGFI-B. Noxious information seems to affect neurons in the contralateral TNC, as well.The distribution study of glutamate-like (GLU-LI) and glutaminase-like (GLNase-LI) immunoreactivity (IR) combined with tracer method showed that trigeminal neurons projecting to the thalamus and cerebellum constitute distinct subpopulations, and that some of these neurons are glutamatergic.Furthermore, NMDAR_1, GluR_2 and GluR_5 subunit mRNAs of glutamate receptor and beta_2,gamma_1 and gamma_2 subunit mRNAs of GABA_A receptor were found in the trigeminal ganglion. These findings indicate that primary sensory neurons are presynapticaly regulated by glutamate and GABA in the TNC. Less

研究麻醉大鼠面部皮肤注射福尔马林致口面部疼痛后三叉神经尾核（TNC）转录因子mRNA和神经肽前体mRNA水平的变化。注射福尔马林后，表达c-fos、NGFI-A和NGFI-B mRNA的神经元数量增加，随后同侧TNC浅层（I和II层）表达前泌乳蛋白（PPG）、前脑啡肽A （PPEA）、前肌啡肽（PPD）和前proachykinin (PPT) mRNA的神经元数量增加。注射福尔马林后6 h， PPG、PPEA、PPD和PPT mRNA标记神经元的数量和强度增加最为明显。c-fos、NGFI-A和NGFI-B mRNA标记神经元数量和强度的增加在2h时最为明显。此外，注射后对侧TNC中含有PPD和PPT mRNA的神经元数量明显增加。对侧TNC中fos样免疫反应阳性神经元数量也增加。这些研究结果表明，疼痛的调节与PPG、PPE、PPD和PPT的产生增加有关，这些基因的转录受c-fos、NGFI-A和NGFI-B等即时早期基因的调控。有害信息似乎也会影响对侧TNC的神经元。结合示踪法对谷氨酸样（GLU-LI）和谷氨酰胺样（GLNase-LI）免疫反应性（IR）分布的研究表明，三叉神经神经元向丘脑和小脑的投射构成了不同的亚群，其中一些神经元是谷氨酸能神经元。此外，在三叉神经节中还发现了谷氨酸受体的NMDAR_1、GluR_2和GluR_5亚基mrna和GABA_A受体的beta_2、gamma_1和gamma_2亚基mrna。这些发现表明，初级感觉神经元在TNC中受谷氨酸和GABA的突触前调控。少