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Cloning of gene involved in facial pain

面部疼痛相关基因的克隆

基本信息

批准号：
07457501
负责人：
UEDA Yutaka
金额：
$ 3.07万
依托单位：
Osaka Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457501/
关键词：
Nociception Cloning Differential display STAT family Cytokine signaling JAK family Trigeminal ganglion Cytokine receptor Pain Inflammation Primary Sensory Neuron Trigeminal spinal nucleus Caudalis pain chronic constristion injury

项目摘要

This research aimed at analizing of pain sensory system. We examined cloning of genes involved in nociception by differential display method. We got a lot of distinctive bands. But we have not got distinctive genes yet. The localization of some cytokine receptors and their downstream intracellular signaling molecules was examined in the rat trigeminal ganglion. Amongcytokine receptor components, we examined signal transduction subchain, gp130, IL-2Rgamma and IL-5Rbeta, which are common to respective groups of cytokine receptors. Most of the sensory ganglion neurons expressed gp130, but not IL-2Rgamma nor IL-5beta. We further examined the localization of Janus kinase family members which were reported to be associated with various kind of cytokine receptors and are thought to be implicated in majorcytokine receptor-signaling pathways. While JAK1 and Tyk 2 were expressed in all type of neurons, JAK2 was predominantly expressed in the small neurons. In addition, JAK3 immunoreactivity was only found in satellite cells. The present results indicate that most of neurons express gp130, and that the localization of JAK family members differs with the cell type. This also suggests that the cytokine receptor-signaling pathway may be different in neuronal and glial cells.

本研究旨在分析痛觉系统。我们用差异显示技术克隆了与伤害感受有关的基因。我们有很多独特的乐队。但我们还没有独特的基因。在大鼠三叉神经节中检测了一些细胞因子受体及其下游细胞内信号分子的定位。在细胞因子受体组分中，我们检测了信号转导亚链gp 130、IL-2 R γ和IL-5 R β，它们是各组细胞因子受体共有的。大多数感觉神经节神经元表达gp 130，但不表达IL-2 R γ或IL-5 β。我们进一步研究了Janus激酶家族成员的定位，据报道，Janus激酶家族成员与各种细胞因子受体相关，并被认为与主要的细胞因子受体信号通路有关。JAK 1和Tyk 2在所有类型的神经元中表达，而JAK 2主要在小神经元中表达。JAK 3仅在卫星细胞中表达。本研究结果表明，大多数神经元表达gp 130，JAK家族成员的定位与细胞类型不同。这也表明细胞因子受体信号通路在神经元和神经胶质细胞中可能是不同的。