Study of neuropathic pain for rats

大鼠神经病理性疼痛的研究

• 批准号: 12671978
• 负责人: UEDA Yutaka
• 金额: $ 1.98万
• 依托单位: Osaka Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671978/
• 关键词: Neuropathic pain; Neural symptom; Behavior test; 神経因性疼痛; サイトカイン

The mechanisms of neuropathic pain are complicated. We investigated differences in three animal models on Sprague-Dawley rats using a behavior test and treatment of cilostazol. The diabetic group received an injection of streptozotocin, and the control rats received an injection of 0.1 M citrate buffer. Operations were done on Chronic Constriction Injury (CCI) rats in the same way as with Bennetts' model. The control rats, which received bilateral sham procedures, were used as non-CCI-CFA rats. Operations were done on Complete Freund's Adjuvant (CFA) rats as prescribed by Eliav's model. Each group of diabetes mellitus (DM) rats, non-DM rats, CCI rats, non-CCI-CFA rats, and CFA rats were subdivided into two groups. One group was given pellets containing cilostazol and the other group was given only pellets. All rats were given behavior tests each week. The tests investigated mechano-allodynia, cold allodynia and mechano-hyperalgesia. Mechano-allodynia was induced in all models. Cilostazol was effective on mechano-allodynia only in the DM group. Cold allodynia and mechano-hyperalgesia was induced only in the CCI group, which showed no change with cilostazol. We defined the mechanisms, duration of effect, and nerves for each models. It is important that we deal with each case individually and that understand mechanisms such as neuropathic pain cannot be treated simply

神经性疼痛的机制是复杂的。我们使用行为测试和西洛他唑治疗研究了Sprague-Dawley大鼠三种动物模型的差异。糖尿病组大鼠注射链脲佐菌素，对照组大鼠注射0.1 M柠檬酸缓冲液。慢性收缩损伤（CCI）大鼠手术方法与Bennetts模型相同。接受双侧假手术的对照大鼠作为非cci - cfa大鼠。按照Eliav模型对完全弗洛伊德氏佐剂（CFA）大鼠进行手术。每组糖尿病（DM）大鼠、非DM大鼠、CCI大鼠、非CCI-CFA大鼠、CFA大鼠再分为两组。一组给予含有西洛他唑的微丸，另一组只给予微丸。每周对所有大鼠进行行为测试。试验研究了机械异常性痛、冷异常性痛和机械痛觉过敏。所有模型均引起机械异常性痛。西洛他唑仅对DM组机械异常性痛有效。只有CCI组引起冷性异常性痛和机械性痛觉过敏，西洛他唑组无改变。我们定义了每种模型的机制、作用持续时间和神经。重要的是，我们要单独处理每个病例，并了解神经性疼痛等机制不能简单地治疗

项目成果

Naomi MORI, Yasushi SAKUMA and Yutaka UEDA: "Differences in neural symptoms for three neuropathic pain models observed with a behavior test"Journal of Osaka Dental University. 30(in printing). (2002)

Naomi MORI、Yasushi SAKUMA 和 Yutaka UEDA：“通过行为测试观察到的三种神经性疼痛模型的神经症状差异”大阪齿科大学学报。

Naomi MORI, Yasushi SAKUMA, Yutaka UEDA: "Differences in neural symptoms for three neuropathic pain models observed with a behavior test"Journal of Osaka Dental University. (発表予定).

Naomi MORI、Yasushi SAKUMA、Yutaka UEDA：“通过行为测试观察到的三种神经性疼痛模型的神经症状差异”大阪齿科大学学报（待出版）。

Naomi MORI, Yasushi SAKUMA, Yutaka UEDA: "Differences in neural symptoms for three neuropathic pain models observed with a behavior test"Journal of Osaka Dental University. 36(1). (2002)

Naomi MORI、Yasushi SAKUMA、Yutaka UEDA：“通过行为测试观察到的三种神经性疼痛模型的神经症状差异”大阪齿科大学学报。