Self Decomposition Products from Radio-labeled Compounds Used as Tracers : Analysis and Control

用作示踪剂的放射性标记化合物的自分解产物：分析和控制

• 批准号: 03453144
• 负责人: KURIHARA Norio
• 金额: $ 1.98万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03453144/
• 关键词: Self-decomposition of ^<35>S-compounds; Volatile radioactive compounds from ^<35>S-methionine; Volatile radioactive compounds from ^<35>S-cysteine; Dimethyl disulfied from ^<35>S-methionine; Self-radiolysis of ^<35>S-compounds; Simultaneous quantitat

1.^<35>S-labeled amino acids of high specific radioactivity are very unstable. The radiochemical purity of L-[^<35>S]methionine, stabilized or not, and of stabilized L-[^<35>S]cysteine was about 90% even at the time of their arrival at the user's laboratory.2.Methyl mercaptan, hydrogen sulfide (or sulfur dioixde) and dimethyl disulfide were the major self-decomposition products generated from L-[^<35>S]methionine without stabilizers. When stabilizers were added, the fraction of methyl mercaptan considerably increased, and the other two decomposition products decreased.3.Methyl mercaptan and hydrogen sulfide (or sulfur dioxide) were the major self decomposition products from L-[^<35>S]cysteine with stabilizers.4.Replacing air in the head space of sample-vials with helium and/or addition of stabilizers can retard the self decomposition, but can not completely stop it. The purity of any labeled compounds of very high specific activity must be carefully examined just before use. The should be used as soon as possible after arrived from th emanufacturer.5.^<35>S, as well as ^3H, ^<14>C, ^<32>P, ^<51>Cr, and ^<125>I retained on a paper disc can be quantified accurately and simultaneously using a flow-type combustion flask followed by counting with a liquid scintillation counter and an NaI(T1) scintillation counter.

1.^ <35>高比放射性的S-标记氨基酸非常不稳定。L-[^<35>S]蛋氨酸（稳定或不稳定）和稳定的L-[^<35>S]半胱氨酸的放射化学纯度即使在它们到达用户实验室时也约为90%。2.在没有稳定剂的情况下，L-[^ S]蛋氨酸产生的主要自分解产物是甲基硫、硫化氢（或二氧化硫）和二甲基二硫<35>。加入稳定剂后，L-[^ S]半胱氨酸的自分解产物主要是甲基硫和硫化氢（或二氧化硫）<35>。但不能完全阻止它。任何比活度很高的标记化合物的纯度在使用前必须仔细检查。产品从制造商处到货后应尽快使用。<35>保留在纸盘上的^3 H、^<14>C、^<32>P、^<51>Cr和^<125>I可以使用流动型燃烧瓶同时精确定量，然后用液体闪烁计数器和NaI（T1）闪烁计数器计数。

项目成果

Kazumi SAITO,Hideo MIYUATAKE,Hiroshige KOBORI,and Norio KURIHARA: "Simultaneous quantitative determination of ^3H, ^<14>C, ^<32>P, ^<35>S, ^<51>Cr and ^<125>I on the surface of a papaer disc using combustion" Health Physics. 65-1. 82-86 (1993)

Kazumi SAITO、Hideo MIYUATAKE、Hiroshige KOBORI 和 Norio KURIHARA：“同时定量测定 ^3H、^<14>C、^<32>P、^<35>S、^<51>Cr 和 ^<125>I

K.Saito et al.: "Simultaneous quantitative determination of ^3H,^<14>C,^<32>P,^<35>S,^<51>Cr and ^<125>I on the surface of a paper disc using combustion" Health Physics. 65. 82-86 (1993)

K.Saito 等人：“同时定量测定纸张表面的 ^3H、^<14>C、^<32>P、^<35>S、^<51>Cr 和 ^<125>I

T.Kato et al.: "Self-decomposition components generated from ^<35>S-labeled amino acids" Applied Radiation and Isotopes. 45 (in press). (1994)

T.Kato等人：“由 35 S标记的氨基酸产生的自分解成分”应用辐射和同位素。

K.Saito et al.: "Simultaneous quantitative determination of ^3H,^<14>C,^<32>P,^<35>S,^<51>Cr and ^<125>I on the surface of a paper disc using combustion" Health Physics. (1993)

K.Saito 等人：“同时定量测定纸张表面的 ^3H、^<14>C、^<32>P、^<35>S、^<51>Cr 和 ^<125>I

Takahisa KATO, Kazumi SAITO, and Norio KURIHARA: "Self-decompositioncomponents generated from ^<35>S-labeled amino acids" Applied Radiation and Isotopes. 45(in press). (1994)

Takahisa KATO、Kazumi SAITO和Norio KURIHARA：“由^ 35 S标记的氨基酸产生的自分解成分”应用辐射和同位素。