Self Decomposition Products from Radio-labeled Compounds Used as Tracers : Analysis and Control

用作示踪剂的放射性标记化合物的自分解产物：分析和控制

• 批准号: 03453144
• 负责人: KURIHARA Norio
• 金额: $ 1.98万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03453144/
• 关键词: Self-decomposition of ^<35>S-compounds; Volatile radioactive compounds from ^<35>S-methionine; Volatile radioactive compounds from ^<35>S-cysteine; Dimethyl disulfied from ^<35>S-methionine; Self-radiolysis of ^<35>S-compounds; Simultaneous quantitat

1.^<35>S-labeled amino acids of high specific radioactivity are very unstable. The radiochemical purity of L-[^<35>S]methionine, stabilized or not, and of stabilized L-[^<35>S]cysteine was about 90% even at the time of their arrival at the user's laboratory.2.Methyl mercaptan, hydrogen sulfide (or sulfur dioixde) and dimethyl disulfide were the major self-decomposition products generated from L-[^<35>S]methionine without stabilizers. When stabilizers were added, the fraction of methyl mercaptan considerably increased, and the other two decomposition products decreased.3.Methyl mercaptan and hydrogen sulfide (or sulfur dioxide) were the major self decomposition products from L-[^<35>S]cysteine with stabilizers.4.Replacing air in the head space of sample-vials with helium and/or addition of stabilizers can retard the self decomposition, but can not completely stop it. The purity of any labeled compounds of very high specific activity must be carefully examined just before use. The should be used as soon as possible after arrived from th emanufacturer.5.^<35>S, as well as ^3H, ^<14>C, ^<32>P, ^<51>Cr, and ^<125>I retained on a paper disc can be quantified accurately and simultaneously using a flow-type combustion flask followed by counting with a liquid scintillation counter and an NaI(T1) scintillation counter.

1.^<35> s标记的高比放射性氨基酸非常不稳定。L-[^<35>S]蛋氨酸，稳定与否，稳定的L-[^<35>S]半胱氨酸的放射化学纯度即使在它们到达用户实验室时也在90%左右。无稳定剂的L-[^<35>S]蛋氨酸产生的自分解产物主要有甲硫醇、硫化氢（或二氧化硫）和二甲基二硫化物。加入稳定剂后，甲基硫醇的分解率显著提高，其他两种分解产物的分解率降低。L-[^<35>S]半胱氨酸在稳定剂作用下的自分解产物主要为甲基硫醇和硫化氢（或二氧化硫）。用氦气和/或添加稳定剂替代样品瓶顶部空气可以延缓自分解，但不能完全阻止自分解。任何具有很高比活性的标记化合物的纯度必须在使用前仔细检查。4 .产品从厂家运来后应尽快使用。^<35>S，以及^3H， ^<14>C, ^<32>P, ^<51>Cr和^<125>I保留在纸盘上，可以使用流动型燃烧烧瓶，然后用液体闪烁计数器和NaI（T1）闪烁计数器计数，同时准确地定量。

项目成果

Kazumi SAITO,Hideo MIYUATAKE,Hiroshige KOBORI,and Norio KURIHARA: "Simultaneous quantitative determination of ^3H, ^<14>C, ^<32>P, ^<35>S, ^<51>Cr and ^<125>I on the surface of a papaer disc using combustion" Health Physics. 65-1. 82-86 (1993)

Kazumi SAITO、Hideo MIYUATAKE、Hiroshige KOBORI 和 Norio KURIHARA：“同时定量测定 ^3H、^<14>C、^<32>P、^<35>S、^<51>Cr 和 ^<125>I

K.Saito et al.: "Simultaneous quantitative determination of ^3H,^<14>C,^<32>P,^<35>S,^<51>Cr and ^<125>I on the surface of a paper disc using combustion" Health Physics. 65. 82-86 (1993)

K.Saito 等人：“同时定量测定纸张表面的 ^3H、^<14>C、^<32>P、^<35>S、^<51>Cr 和 ^<125>I

T.Kato et al.: "Self-decomposition components generated from ^<35>S-labeled amino acids" Applied Radiation and Isotopes. 45 (in press). (1994)

T.Kato等人：“由 35 S标记的氨基酸产生的自分解成分”应用辐射和同位素。

K.Saito et al.: "Simultaneous quantitative determination of ^3H,^<14>C,^<32>P,^<35>S,^<51>Cr and ^<125>I on the surface of a paper disc using combustion" Health Physics. (1993)

K.Saito 等人：“同时定量测定纸张表面的 ^3H、^<14>C、^<32>P、^<35>S、^<51>Cr 和 ^<125>I

Takahisa KATO, Kazumi SAITO, and Norio KURIHARA: "Self-decompositioncomponents generated from ^<35>S-labeled amino acids" Applied Radiation and Isotopes. 45(in press). (1994)

Takahisa KATO、Kazumi SAITO和Norio KURIHARA：“由^ 35 S标记的氨基酸产生的自分解成分”应用辐射和同位素。