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Molecular Construction on the Formation of Cell Wall by Immunofluorescence Microscopy

免疫荧光显微镜下细胞壁形成的分子构建

基本信息

批准号：
03454080
负责人：
ITOH Takao
金额：
$ 0.32万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

项目摘要

We examined the arrangement of cortical MTs in the elongating epicotyl of Aesculus turbinata by immunofluorescence microscopy using cryosections. Under both dark and light-dark growth conditions, the arrangement of MTs was associated with the rates of elongation and differed according to the region in the same epicotyl. The present results indicate that the change in the arrangement of cortical MTs from transverse to oblique or longitudinal ones occurs in the cortex before elongation stops. Orientation of MTs in both growth conditions seems to be steeper in the outer than in the inner parenchyma cells and to change earlier in the light-dark grown epicotyl.The architecture of the primary wall in poplar suspension-cultured cells was observed after rapid freezing and deep etching techniques before and after the sequential extraction of cell wall polysaccharides.The cell wall architecture was also examined after the treatment by pectin-degrading enzymes. Our observations showed that the inter-fibrillar spaces or pore size increases after extraction of pectins both with chemical and enzymatic treatments. The ordered spacing of cellulose microfibrils was only slightly altered after 4 % KOH-treatment and drastically altered after 24 % KOH-treatment. These results suggest that the hemicellulose, possibly xyloglucan, may have apossible role in maintaining the three-dimensional conformation by inter-fibrillar polysaccharide linkages in the cell wall of this dicotyledonous species.

利用免疫荧光显微镜观察了七叶树（Aesculus turbinata）上胚轴皮层MT的排列。在黑暗和光暗生长条件下，MT的排列与伸长速率有关，并且在同一上胚轴中根据区域而不同。结果表明，在延长停止前，皮质MT的排列发生了由横向向斜向或纵向的变化。在两种生长条件下，MT在薄壁细胞中的取向都是外薄壁细胞比内薄壁细胞更陡，且在上胚轴生长过程中，MT的取向变化更早。在连续提取细胞壁多糖前后，采用快速冷冻和深蚀刻技术，观察了白杨悬浮培养细胞初生壁的构型。降解酶我们的观察表明，纤维间的空间或孔径增加后，提取果胶与化学和酶处理。纤维素微纤丝的有序间距在4%KOH处理后仅轻微改变，而在24%KOH处理后急剧改变。这些结果表明，半纤维素，可能是木葡聚糖，可能有apossible的作用，在维持三维构象的纤维间多糖连接在这个双子叶植物的细胞壁。