Molecular Construction on the Formation of Cell Wall by Immunofluorescence Microscopy

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We examined the arrangement of cortical MTs in the elongating epicotyl of Aesculus turbinata by immunofluorescence microscopy using cryosections. Under both dark and light-dark growth conditions, the arrangement of MTs was associated with the rates of elongation and differed according to the region in the same epicotyl. The present results indicate that the change in the arrangement of cortical MTs from transverse to oblique or longitudinal ones occurs in the cortex before elongation stops. Orientation of MTs in both growth conditions seems to be steeper in the outer than in the inner parenchyma cells and to change earlier in the light-dark grown epicotyl.The architecture of the primary wall in poplar suspension-cultured cells was observed after rapid freezing and deep etching techniques before and after the sequential extraction of cell wall polysaccharides.The cell wall architecture was also examined after the treatment by pectin-degrading enzymes. Our observations showed that the inter-fibrillar spaces or pore size increases after extraction of pectins both with chemical and enzymatic treatments. The ordered spacing of cellulose microfibrils was only slightly altered after 4 % KOH-treatment and drastically altered after 24 % KOH-treatment. These results suggest that the hemicellulose, possibly xyloglucan, may have apossible role in maintaining the three-dimensional conformation by inter-fibrillar polysaccharide linkages in the cell wall of this dicotyledonous species.