Prediction of recurrence or metastasis of malignant tumors by assessment of their growth abilities and its clinical application

最近研究表明，细胞增殖活性比DNA倍性更有意义。肿瘤细胞的增殖状态大致由几个因素决定，包括有丝分裂指数、S期分数、标记指数和肿瘤集落形成。然而，这些因素被认为不如肿瘤分期重要。组织培养技术的发展使新鲜实体瘤细胞在半固体培养基中增殖成为可能。在我们最初开发的胸苷掺入试验中，可以测量肿瘤细胞对氚化胸苷的掺入。为探讨胃癌和结直肠癌患者~（331）TdR摄取的临床病理学相关性及其预后价值，对173例可手术胃癌和127例结直肠癌患者进行了半固体培养的肿瘤细胞~（331）TdR摄取的测定，并通过单因素和多因素分析比较了各参数对生存率的影响。无论是胃癌还是结直肠癌，高摄取组患者的预后都比低摄取组差。多变量分析显示，胸苷摄取是我们研究的胃癌或结直肠癌患者中与生存率密切相关的变量之一。因此，可以得出结论，肿瘤细胞的胸苷摄取是一个独立的预后指标，这可能是有用的选择胃癌和结直肠癌患者谁wouldbenefitil从更积极的治疗。

It was recently demonstrated that proliferative activity is prognostically more significant than DNA ploidy petterns. The proliferative status of tumor cells has been roughly determined by several factors, including the mitotic index, S-phase fraction, labeling index and tumor colony formation. However, those fasctors have been fegarded as being less important than tumor staging. Advances in tissue culture techniques ha have made it possible for cells from fresh solid tumors to proliferate in semisolid medium. In a thymidine incorporation assay, which we originally developed, it is possible to measure the incorporation of tritiated thymidine by tumor cells. The scope of this study is to evaluate the clonicopathological correlations and the prognostic value of tritiated thymidine uptake in gastric and colorectal cancer patients.In 173 patients with operable gastric cancer and in 127 patients with colorectal carcinoma, thymidine uptake by tumor cells cultured in a semi solid medium was medsured and was compared the influence of various parameters on survival by**ivariate and multivariate analysis. Patients in the high-uptake had a poorerprognosis than those in the low-uptake, both with gastric and colorectal cancer. Mulativariate analysis showed that thymidine uptake was one of the variables strongly associated with survival in our study population with gastric or colorectal carcinoma. Thus, it is concluded that thymidine uptake by tumor cells is an independent prognostic indicator which may be useful for selecting patients with gastric and colorectal carcinoma who wouldbenetil from more aggressive treatment.