Prediction of recurrence or metastasis of malignant tumors by assessment of their growth abilities and its clinical application

通过评估恶性肿瘤的生长能力来预测其复发或转移及其临床应用

• 批准号: 03454316
• 负责人: TANIGAWA Nobuhiko
• 金额: $ 2.5万
• 依托单位: Fukui Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454316/
• 关键词: Malignat tumor; Prediction of prognosis; In vitro growth ability; ^3H thymidine uptake; CPM; Gastric cancer; Colorectal cancer; Multivariate analysis; ^3Hサイミディン摂取能; In vivo増殖能; コロニ-形成能; Tpot; 腫瘍倍加時間

It was recently demonstrated that proliferative activity is prognostically more significant than DNA ploidy petterns. The proliferative status of tumor cells has been roughly determined by several factors, including the mitotic index, S-phase fraction, labeling index and tumor colony formation. However, those fasctors have been fegarded as being less important than tumor staging. Advances in tissue culture techniques ha have made it possible for cells from fresh solid tumors to proliferate in semisolid medium. In a thymidine incorporation assay, which we originally developed, it is possible to measure the incorporation of tritiated thymidine by tumor cells. The scope of this study is to evaluate the clonicopathological correlations and the prognostic value of tritiated thymidine uptake in gastric and colorectal cancer patients.In 173 patients with operable gastric cancer and in 127 patients with colorectal carcinoma, thymidine uptake by tumor cells cultured in a semi solid medium was medsured and was compared the influence of various parameters on survival by**ivariate and multivariate analysis. Patients in the high-uptake had a poorerprognosis than those in the low-uptake, both with gastric and colorectal cancer. Mulativariate analysis showed that thymidine uptake was one of the variables strongly associated with survival in our study population with gastric or colorectal carcinoma. Thus, it is concluded that thymidine uptake by tumor cells is an independent prognostic indicator which may be useful for selecting patients with gastric and colorectal carcinoma who wouldbenetil from more aggressive treatment.

最近研究表明，细胞增殖活性比DNA倍性更有意义。肿瘤细胞的增殖状态大致由几个因素决定，包括有丝分裂指数、S期分数、标记指数和肿瘤集落形成。然而，这些因素被认为不如肿瘤分期重要。组织培养技术的发展使新鲜实体瘤细胞在半固体培养基中增殖成为可能。在我们最初开发的胸苷掺入试验中，可以测量肿瘤细胞对氚化胸苷的掺入。为探讨胃癌和结直肠癌患者~（331）TdR摄取的临床病理学相关性及其预后价值，对173例可手术胃癌和127例结直肠癌患者进行了半固体培养的肿瘤细胞~（331）TdR摄取的测定，并通过单因素和多因素分析比较了各参数对生存率的影响。无论是胃癌还是结直肠癌，高摄取组患者的预后都比低摄取组差。多变量分析显示，胸苷摄取是我们研究的胃癌或结直肠癌患者中与生存率密切相关的变量之一。因此，可以得出结论，肿瘤细胞的胸苷摄取是一个独立的预后指标，这可能是有用的选择胃癌和结直肠癌患者谁wouldbenefitil从更积极的治疗。

项目成果

Nobuhiko Tanigawa,Hideki Morimoto,Naochika Dohmae,Takumi Shimomatsuya,Kohji Takahashi and Ryusuke Muraoka: "In vitro growth ability and chemosensitivity of gastric and colorectal cancer cells assessed with the human tumour clonogenic assay and the thymidi

Nobuhiko Tanikawa、Hideki Morimoto、Naochika Dohmae、Takumi Shimomatsuya、Kohji Takahashi 和 Ryusuke Muraoka：“利用人类肿瘤克隆形成试验和胸腺嘧啶核苷评估胃癌和结直肠癌细胞的体外生长能力和化疗敏感性

谷川 允彦: "消化器癌転移病変の特性" 日本消化器外科学会雑誌. 24. 1101-1106 (1991)

Masahiko Tanikawa：“胃肠癌转移病变的特征”日本胃肠外科学会杂志 24. 1101-1106 (1991)。

Takumi Shimomatsuya: "Proliferative activity of human tumors:assessment uisng bromodeoxyuridine and flow cytometry" Jpn J Cancer Res. 82. 357-362 (1991)

Takumi Shimomatsuya：“人类肿瘤的增殖活性：使用溴脱氧尿苷和流式细胞术进行评估”Jpn J Cancer Res。

Hideki Morimoto, Nobuhiko Tanigawa: "The significance of postoperative early chemotherapy for locogegional lymph node metastasis of gastric and colorectal cancer." Oncology. 48. 210-214 (1991)

Hideki Morimoto、Nobuhiko Tanikawa：“术后早期化疗对胃癌和结直肠癌局部淋巴结转移的意义”。

Hideki Morimoto,: "The significance of postoperative early chemo-therapy for locoregional lymph node metastasis of gastric and colorectal." Oncology. 48. 210-214 (1991)

Hideki Morimoto：“术后早期化疗对胃和结直肠局部淋巴结转移的意义。”