Optical imaging of neural activity in transient ischemia and plasticity in cortex

短暂性缺血神经活动和皮质可塑性的光学成像

基本信息

  • 批准号:
    03454345
  • 负责人:
  • 金额:
    $ 4.03万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
  • 财政年份:
    1991
  • 资助国家:
    日本
  • 起止时间:
    1991 至 1992
  • 项目状态:
    已结题

项目摘要

The spatio-temporal pattern of sound-evoked neural activity in the mammalian auditory cortex was studied by optical recording with aid of voltage-sensitive dye (RH 795). This method was applied to observation of transient ischemic changes in the cortical activity. Changes in light intensity at 620 nm induced sounds at various frequency and pressure levels were recorded with a 12 x 12 array of photodiodes. The amplitude of the responses were displayed as sequential two-dimensional images. Tonotopical organization was found in the multi-fields of the auditory cortex. However, frequency representation through the functional organization was transient. The focal activity that began in the primary field propagated dorsally along the iso-frequency bands. In the rats auditory cortex, the optical signals induced by sounds were disappeared by 1 min after induction of ischemia. During the 30 min of ischemia, no components of the responses were observed. After recirculation the responses from 144 sites appeared again. However the pattern of the responses after the transient ischemia was significantly different from the control. Especially the later component did not recover to the preischemic level. Although functional organization appeared, but vulnerable regions were found within the organization where the optical responses ded not return to the control Our results suggest that the functional changes in the auditory cortex precede the post-ischemic neuronal death in those cerebral regions. In guinea pigs, there were some cases which showed an increase in the magnitude of optical responses from the cortex by transient ischemia. The optical recording method has the advantage of real-time imaging of two-dimensional cortical activity and will be used widely in the future.
本文用电压敏感染料(RH 795)光记录法研究了哺乳动物听皮层内声诱发神经活动的时空模式。该方法用于观察短暂性脑缺血时皮层活动的变化。用12 × 12光电二极管阵列记录在不同频率和压力水平下620 nm处诱导的声音的光强度变化。反应的幅度显示为连续的二维图像。在听觉皮层的多个领域中发现了音调组织。然而,通过功能组织的频率表示是短暂的。在初级场开始的局灶性活动沿等频带向背侧沿着传播。在大鼠听皮层,声音诱导的光信号在缺血后1min消失。在30分钟的缺血期间,没有观察到反应的成分。再循环后,144个位点的反应再次出现。然而,短暂缺血后的反应模式与对照组有显着差异。尤其是后一种成分没有恢复到缺血前水平。虽然出现了功能性组织,但在这些组织中发现了视觉反应不能恢复到对照的脆弱区域。我们的结果表明,听觉皮层的功能变化先于这些脑区的缺血后神经元死亡。在豚鼠中,有一些情况下,表现出从皮层的光学响应的幅度增加,由短暂缺血。光学记录方法具有实时成像二维皮层活动的优点,具有广阔的应用前景。

项目成果

期刊论文数量(49)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Bonnekoh,P.,Kuroiwa,T.,Oschilies,U.and Hossmann,K.-A.: "Barbiturate promotes post-ischemic reaggregation of polyribosomes in gerbil hippocampus." Neurosci.Lett.146. 75-78 (1992)
Bonnekoh,P.、Kuroiwa,T.、Oschilies,U. 和 Hossmann,K.-A.:“巴比妥促进沙鼠海马缺血后多核糖体的重新聚集。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Kuroiwa,T.,Yokofujita,J.,Kaneko,H.and Okeda,R.: "Microradiographic examination of edema fluid distribution in the cat brain after cryogenic injury." Intracranial Pressure VIII,Springer-Verlag,in press. (1993)
Kuroiwa,T.、Yokofujita,J.、Kaneko,H. 和 Okeda,R.:“冷冻损伤后猫脑中水肿液分布的显微放射检查。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
J.Horikawa: "Spatial response patterns of auditory cortical neurons measured with a 16ーchannel electrode array." Jpn.J.Physiol.41. 239 (1991)
J.Horikawa:“用 16 通道电极阵列测量听觉皮层神经元的空间反应模式。”Jpn.J.Physiol.41 (1991)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
市来 浩人,黒岩 俊彦,谷口 郁雄: "脳虚血による聴性誘発電位の変化とその病理ー虚血時間の影響ー" Audiol.Jpn.35. 403-404 (1992)
Hiroto Ichiki、Toshihiko Kuroiwa、Ikuo Taniguchi:“脑缺血引起的听觉诱发电位的变化及其病理学 - 缺血时间的影响”Audiol.Jpn.35 (1992)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

TANIGUCHI Ikuo其他文献

TANIGUCHI Ikuo的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('TANIGUCHI Ikuo', 18)}}的其他基金

A NEUROPHYSIOLOGICAL STUDY ON COCHLEAR IMPLANTATION BY OPTICAL IMAGING
人工耳蜗植入的神经生理学光学成像研究
  • 批准号:
    06454484
  • 财政年份:
    1994
  • 资助金额:
    $ 4.03万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Post-lesion plasticity in the auditory central nervous system
听觉中枢神经系统损伤后可塑性
  • 批准号:
    63570805
  • 财政年份:
    1988
  • 资助金额:
    $ 4.03万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似海外基金

Mrgprb2 mediated neuroinflammation after cerebral ischemia
Mrgprb2介导脑缺血后的神经炎症
  • 批准号:
    10644182
  • 财政年份:
    2023
  • 资助金额:
    $ 4.03万
  • 项目类别:
Sexually Dimorphic Amygdala Dysfunction in a Mouse Model of Global Cerebral Ischemia
全脑缺血小鼠模型中的性二形性杏仁核功能障碍
  • 批准号:
    10828626
  • 财政年份:
    2023
  • 资助金额:
    $ 4.03万
  • 项目类别:
Astrocyte-specific TLR4 signaling and Blood Brain Barrier permeability following acute focal cerebral ischemia
急性局灶性脑缺血后星形胶质细胞特异性 TLR4 信号传导和血脑屏障通透性
  • 批准号:
    10572987
  • 财政年份:
    2023
  • 资助金额:
    $ 4.03万
  • 项目类别:
ContinuOuS Monitoring Tool for Delayed Cerebral IsChemia (COSMIC)
迟发性脑缺血持续监测工具 (COSMIC)
  • 批准号:
    10736589
  • 财政年份:
    2023
  • 资助金额:
    $ 4.03万
  • 项目类别:
Fluoxetine recovery of synaptic dysfunction following juvenile global cerebral ischemia
氟西汀恢复青少年全脑缺血后突触功能障碍
  • 批准号:
    10509753
  • 财政年份:
    2022
  • 资助金额:
    $ 4.03万
  • 项目类别:
Novel neuroprotective therapy by inhibition of ferroptosis in cerebral ischemia-reperfusion injury
通过抑制脑缺血再灌注损伤中铁死亡的新型神经保护疗法
  • 批准号:
    22K16676
  • 财政年份:
    2022
  • 资助金额:
    $ 4.03万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Discovery of Plasma Biomarkers of Delayed Cerebral Ischemia after Subarachnoid Hemorrhage
蛛网膜下腔出血后迟发性脑缺血血浆生物标志物的发现
  • 批准号:
    10358411
  • 财政年份:
    2022
  • 资助金额:
    $ 4.03万
  • 项目类别:
Discovery of Plasma Biomarkers of Delayed Cerebral Ischemia after Subarachnoid Hemorrhage
蛛网膜下腔出血后迟发性脑缺血血浆生物标志物的发现
  • 批准号:
    10589874
  • 财政年份:
    2022
  • 资助金额:
    $ 4.03万
  • 项目类别:
Estrous cycle stage at the time of global cerebral ischemia: Implications for hypothalamic-pituitary-gonadal axis function and estrous cycle regularity
全脑缺血时的动情周期阶段:对下丘脑-垂体-性腺轴功能和动情周期规律的影响
  • 批准号:
    572356-2022
  • 财政年份:
    2022
  • 资助金额:
    $ 4.03万
  • 项目类别:
    Alexander Graham Bell Canada Graduate Scholarships - Master's
Innovative Research of Neuroprotection for Delayed Cerebral Ischemia Associated with Cerebral Vasospasm after Subarachnoid Hemorrhage using Novel Hemoglobin-based Oxygen Carrier
新型血红蛋白氧载体对蛛网膜下腔出血后迟发性脑血管痉挛相关迟发性脑缺血的神经保护创新研究
  • 批准号:
    22K09275
  • 财政年份:
    2022
  • 资助金额:
    $ 4.03万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了