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A sperm motility inhibitor of human seminal plasma

人精浆精子活力抑制剂

基本信息

批准号：
03454390
负责人：
IWAMOTO Teruaki
金额：
$ 4.1万
依托单位：
St.Marianna University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454390/
关键词：
Seminal plasma sperm motility inhibitor Demenbranated spermatozoa Dynein ATPase Dynein arm Seminal plasma Seminal vesicle fluid Seminal vesicle Astchenozoospermia 精子細胞抽出液精子 dynein ATPase

项目摘要

1. A seminal plasma sperm motility inhibitor (SPMI) from boar was purified. The molecular weight of SPMI in native conditions has been estimated at 50kD by molecular sieving, but polypeptides with molecular weights of 14,16 and 18kD were observed following SDS-PAGE in denanring condition. The observation that SPMI effects on motility of demenbranated spermatozoa are reversed by Mg.ATP and that SPMI inhibited bull dynein ATPase suggest that this protein blocks the motility of demenbranated spermarozoa by interfering with dynein arm function.2. The origin of human SPMI was investigated SPMI was present in significant amounts only in seminal vesical fluids (SVF). The level of biological activity in this fluid was 13.6-fold higher than in seminal plasma (SP). However, the concentration of motility inhibitor, as measured by ELISA, using an antibody generated against the motility inhibitor purified from human SP, was only 1.54-fold higher than in SP.The data suggest that SPMI originates from SVF and that it is processed into a nine-fold less biologically active form when SVF is mixed with other accessory gland secretions at ejaculation.3. The origin f boar SPMI was investigated. By immunohistochemical detection at the electron microscope level, SPMI appeared to be present only on the surface of the secretory cells in the seminal vesicles(SV). The data indicate that SPMI originates from single tissue, the SV, and suggest that only the mature form present on the luminal surface of the gland can react with the antibody generated from rabbits immunized with the serected form of SP.4. SPMI biological assay in the SP from infertile patients was measured. No significant correlation was observed between SPMI activity and sperm motility. This data suggest that the poor motile sperm may be caused by high permeability of the sperm membrane to SPMI.

1. 纯化公猪精浆精子活力抑制剂（SPMI）。通过分子筛估计天然条件下SPMI的分子量为50kD，但在脱南环条件下进行SDS-PAGE后观察到分子量为14,16和18kD的多肽。 Mg.ATP可逆转SPMI对脱膜精子运动的影响，并且SPMI抑制公牛动力蛋白ATP酶，表明该蛋白通过干扰动力蛋白臂功能而阻断脱膜精子的运动。 2．研究了人类 SPMI 的起源，SPMI 仅在精囊液 (SVF) 中大量存在。该液体中的生物活性水平比精浆 (SP) 高 13.6 倍。然而，使用从人 SP 纯化的运动抑制剂产生的抗体，通过 ELISA 测量，运动抑制剂的浓度仅比 SP 中高 1.54 倍。数据表明，SPMI 源自 SVF，当 SVF 在射精时与其他附属腺分泌物混合时，它被加工成生物活性低 9 倍的形式。3。对公猪 SPMI 的起源进行了调查。通过电镜水平的免疫组织化学检测，SPMI似乎仅存在于精囊（SV）分泌细胞的表面。数据表明SPMI源自单一组织SV，并表明只有腺体腔表面上存在的成熟形式才能与用SP.4的分离形式免疫的兔子产生的抗体反应。 SPMI 生物测定对不孕患者的 SP 进行了测量。 SPMI 活性和精子活力之间没有观察到显着相关性。该数据表明，精子活动能力差可能是由于精子膜对 SPMI 的高渗透性造成的。