Establishment of the laboratory procedure to judge the quality of the sperm which assumed seminal vesicle protein a marker

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For a diagnosis of the asthenozoospermia, the only method is conventional measurement of sperm motility rate under a microscope. We investigate the association between Semenogelin(Sg) and spermatozoa in infertile men with asthenozoospermia using a anti-Sg antibody. Spermatozoa, washed by Percoll density gradients, were immunostained with anti- Sg antibody and subjected to flow cytometric analysis. The proportion of spermatozoa labeled with the antibody and the average intensity of fluorescence labeling per spermatozoa were analyzed in relation to the parameters used for semen analysis. A significant negative correlation was found between sperm motility and the proportion and intensity of labeling. The method presented in this study allows reproducible and objective assessment of the asthenozoospermia.