A sperm motility inhibitor of seminal plasma.

精浆的精子活力抑制剂。

• 批准号: 05044189
• 负责人: IWAMOTO Teruaki
• 金额: $ 2.3万
• 依托单位: St.Marianna University Scholl of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05044189/
• 关键词: seminal plaswa motility inhibitor; seminal vesicle protein; seminal plasma demembranated reactivated spermatozoa; Spermadhesin; 精子無力症; Spermadhesin; 精嚢

The seminal plasma motility inhibitor (SPMI) has recently been purified from boar seminal plasma where its molecular weight has estimated at 50,000 by molecular sieving but three peptides of 14k, 16k, 18k were detected after SDSPAGE in denaturing conditions. We succeeded the cloning of boar 14k SPMI cDNA gene. Nucreotide sequence analysis of the 645-base pair SPMI cDNA predicts a code peptide of 137 amino acid residues which includes a 21residue signal peptide and a 116-residue secreted protein.The levels of SPMI gene expression were determined by Northrn blot analysis using the probe DNAe encoding the open reading frame of the cloned SPMI cDNA gene. It was soon noticed that the boar seminal vesicle is extrordinarily abundant in SPMI mRNA (after 3 hrs expousure). In contrast, there was no detectable level of SPMI expression in the testis, epididymis, prostate, bulbourethral gland, urinary bladder, liver, spleen, as well as in the reproductive organs of the sow and also human seminal vesicles. Thus it would appear that the expression of the SPMI gene is highly specific to the seminal vesiclesWe succeeded the expression of protein from the cloned SMPI cDNA gene.Expressed protein reacted against SPMI antibody by western blotting. Although this expressed SPMI protein was able to solubilize with some buffers, SPMI biological activity was lost. Further studies needs to resolve this problem.The causes of asthenozoospermia (patient with poor motile sperm) were still not clear. We already observed that the inhibitory capacity of seminal plasma from patients with poor sperm motility was not significantly different from that of seminal plasma from normal fertile men with good sperm motility. Now we are studying whether a motility inhibitor within poor motile spermatozoa present and is identical to SPMI.But, presently, we can not show

精浆运动抑制剂（SPMI）最近从猪精浆中纯化出来，通过分子筛估计其分子量为50,000，但在变性条件下SDSPAGE后检测到14k， 16k， 18k的三种肽。成功克隆了公猪14k SPMI cDNA基因。对645个碱基对的SPMI cDNA进行核苷酸序列分析，得到一个包含137个氨基酸残基的编码肽，其中包括21个残基信号肽和116个残基分泌蛋白。利用克隆的SPMI cDNA开放阅读框的探针dna编码，通过northern blot检测SPMI基因的表达水平。很快就注意到（暴露3小时后）公猪精囊中SPMI mRNA异常丰富。相比之下，SPMI在母猪的睾丸、附睾、前列腺、尿道球腺、膀胱、肝脏、脾脏以及生殖器官和人类精囊中均未检测到表达水平。因此，SPMI基因的表达对精囊具有高度的特异性。我们成功地从克隆的SMPI cDNA基因中表达了蛋白。western blotting检测表达蛋白与SPMI抗体反应。虽然这种表达的SPMI蛋白能够被一些缓冲液溶解，但SPMI的生物活性丧失。需要进一步的研究来解决这个问题。弱精子症（精子活动能力差的患者）的病因尚不清楚。我们已经观察到，精子活力差的患者的精浆抑制能力与精子活力好的正常可育男性的精浆抑制能力没有显著差异。现在我们正在研究运动能力差的精子中是否存在一种运动抑制剂，并且与SPMI相同。但是，目前，我们不能显示

项目成果

T.Iwamoto, H.Tanaka, T.Osada: "Biological activity of seminal plasma motility inhibitor in infertile patients." Jpn.J.Fertil.Steril. 38. 215-219 (1993)

T.Iwamoto、H.Tanaka、T.Osada：“精浆运动抑制剂在不孕患者中的生物活性。”

T.Iwamoto et.al.: "Origin of a sperm motility inhibitor from boar seminal plasma." Molecular Reproduction and Development. 36. 475-481 (1993)

T.Iwamoto 等人：“来自公猪精浆的精子活力抑制剂的起源。”

T.Iwamoto et al.: "Origin of a sperm motility inhibicor from boar seminal plasma." Molecular Reproduction and Development. 36. 475-481 (1993)

T.Iwamoto 等人：“来自公猪精浆的精子活力抑制剂的起源。”