THE INVESTIGATION OF CELL CYCLE REGULATORY GENES IN MAMMALIAN CELL

哺乳动物细胞细胞周期调控基因的研究

• 批准号: 03454560
• 负责人: NISHIMOTO Takeharu
• 金额: $ 3.97万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-03454560/
• 关键词: CELL CYCLE; CCG1; RCC1; RPS4X; DAD1; CDC25; PCC; G2-BLOCK; 温度感受性変異; DADl; CCGl; 温度感受性変異株; CCG2(RPS4X); アポトーシス; CCG2

We have isolated temperature sensitive mutants for cell growth from the BHK21 cell lines derived from golden hamsters. Using these mutants as a receipient of DNA-mediated gene transfer, we have been cloning human genes complementing ts mutation. So far, we have cloned human genes, RCC1, CCG1, RPS4X and DAD1. Under the present research project, we have cloned the RPS4X and DAD1 genes, and characterized the function of RCC1 and CCG1 gene-products. Additionaly, we have proved that the human cDNA encoding ubiquitin-activating enzyme E1 complements tsBN75, a temperature sensitive mutant of the BHK21 cell line which shows G2 block at restrictive temperature.

我们已经从来自金黄仓鼠的BHK 21细胞系中分离出了用于细胞生长的温度敏感突变体。使用这些突变体作为DNA介导的基因转移的接受者，我们一直在克隆补充其突变的人类基因。到目前为止，我们已经克隆了人类基因，RCC1，CCG1，RPS4X和DAD 1。本研究计划克隆了RPS4X和DAD 1基因，并对RCC1和CCG 1基因产物的功能进行了研究。此外，我们还证明了编码泛素激活酶E1的人cDNA与BHK 21细胞系的温度敏感突变体tsBN 75互补，tsBN 75在限制性温度下显示G2阻滞。

项目成果

M.Watanabe: "Molecular cloning of the human gene,CCG2,that complements the BHK-derived tempaerature-sensitive cell cycle mutant tsBN63:identity of CCG2 with the human X chromosomal SCAR/RPS4X gene." J.Cell.Science. 100. 35-43 (1991)

M.Watanabe：“人类基因 CCG2 的分子克隆，它补充了 BHK 衍生的温度敏感细胞周期突变体 tsBN63：CCG2 与人类 X 染色体 SCAR/RPS4X 基因的同一性。”

Klebe,C.,Nishimoto,T.,et al.,: "Functional expression in E.coli of the mitotic regulator proteins p24ran and p45RCC1 and fluorescence measurements of their interaction." Biochemistry. 32. 11923-11928 (1993)

Klebe,C.、Nishimoto,T. 等人：“有丝分裂调节蛋白 p24ran 和 p45RCC1 在大肠杆菌中的功能表达以及它们相互作用的荧光测量。”

M.Ohtsubo, T.Yoshida, H.Seino, H.Nishitani, K.Clark, G.Sprague, M.Frasch and T.Nishimoto.: "Mutation of the hamster cell cycle gene RCC1 is complemented by the homologous genes of Drosophila and S.cerevisiae." EMBO J. 10. 1265-1273 (1991)

M.Ohtsubo、T.Yoshida、H.Seino、H.Nishitani、K.Clark、G.Sprague、M.Frasch 和 T.Nishimoto.：“仓鼠细胞周期基因 RCC1 的突变得到果蝇同源基因的补充

T.Sekiguchi, Y.Nohiro, Y.Nakamura, N.Hisamoto and T.Nishimoto.: "The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein. Mol. cell. biol. 11, 3317-3325." Mol. cell. Biol. 11. 3317-3325 (1991)

T.Sekiguchi、Y.Nohiro、Y.Nakamura、N.Hisamoto 和 T.Nishimoto.：“人类 CCG1 基因对于 G1 期的进展至关重要，编码 210 千道尔顿的核 DNA 结合蛋白。Mol. 细胞。

T.Sekiguchi: "The human CCG1 gene,essential for progression of the G1 phase,encodes a210ーkDa Nuclear DNAーbinding protein." Mo1.Cell.Bio1. 11. 3317-3325 (1991)

T. Sekiguchi：“人类 CCG1 基因对于 G1 期的进展至关重要，编码 210 kDa 核 DNA 结合蛋白。Mo1.Cell.Bio1。”