THE INVESTIGATION OF CELL CYCLE REGULATORY GENES IN MAMMALIAN CELL
THE INVESTIGATION OF CELL CYCLE REGULATORY GENES IN MAMMALIAN CELL
批准号:
03454560
负责人:
NISHIMOTO Takeharu
金额:
$3.97万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (B)
财政年份:
1991
资助国家:
日本
项目状态:
已结题
起止时间:
1991 至 1993
中文摘要
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英文摘要
We have isolated temperature sensitive mutants for cell growth from the BHK21 cell lines derived from golden hamsters. Using these mutants as a receipient of DNA-mediated gene transfer, we have been cloning human genes complementing ts mutation. So far, we have cloned human genes, RCC1, CCG1, RPS4X and DAD1. Under the present research project, we have cloned the RPS4X and DAD1 genes, and characterized the function of RCC1 and CCG1 gene-products. Additionaly, we have proved that the human cDNA encoding ubiquitin-activating enzyme E1 complements tsBN75, a temperature sensitive mutant of the BHK21 cell line which shows G2 block at restrictive temperature.
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M.Watanabe: "Molecular cloning of the human gene,CCG2,that complements the BHK-derived tempaerature-sensitive cell cycle mutant tsBN63:identity of CCG2 with the human X chromosomal SCAR/RPS4X gene." J.Cell.Science. 100. 35-43 (1991)
M.Watanabe:“人类基因 CCG2 的分子克隆,它补充了 BHK 衍生的温度敏感细胞周期突变体 tsBN63:CCG2 与人类 X 染色体 SCAR/RPS4X 基因的同一性。”
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通讯作者:
Klebe,C.,Nishimoto,T.,et al.,: "Functional expression in E.coli of the mitotic regulator proteins p24ran and p45RCC1 and fluorescence measurements of their interaction." Biochemistry. 32. 11923-11928 (1993)
Klebe,C.、Nishimoto,T. 等人:“有丝分裂调节蛋白 p24ran 和 p45RCC1 在大肠杆菌中的功能表达以及它们相互作用的荧光测量。”
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T.Sekiguchi: "The human CCG1 gene,essential for progression of the G1 phase,encodes a210ーkDa Nuclear DNAーbinding protein." Mo1.Cell.Bio1. 11. 3317-3325 (1991)
T. Sekiguchi:“人类 CCG1 基因对于 G1 期的进展至关重要,编码 210 kDa 核 DNA 结合蛋白。Mo1.Cell.Bio1。”
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通讯作者:
M.Ohtsubo, T.Yoshida, H.Seino, H.Nishitani, K.Clark, G.Sprague, M.Frasch and T.Nishimoto.: "Mutation of the hamster cell cycle gene RCC1 is complemented by the homologous genes of Drosophila and S.cerevisiae." EMBO J. 10. 1265-1273 (1991)
M.Ohtsubo、T.Yoshida、H.Seino、H.Nishitani、K.Clark、G.Sprague、M.Frasch 和 T.Nishimoto.:“仓鼠细胞周期基因 RCC1 的突变得到果蝇同源基因的补充
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通讯作者:
T.Sekiguchi, Y.Nohiro, Y.Nakamura, N.Hisamoto and T.Nishimoto.: "The human CCG1 gene, essential for progression of the G1 phase, encodes a 210-kilodalton nuclear DNA-binding protein. Mol. cell. biol. 11, 3317-3325." Mol. cell. Biol. 11. 3317-3325 (1991)
T.Sekiguchi、Y.Nohiro、Y.Nakamura、N.Hisamoto 和 T.Nishimoto.:“人类 CCG1 基因对于 G1 期的进展至关重要,编码 210 千道尔顿的核 DNA 结合蛋白。Mol. 细胞。
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共 25 条
Regulation of cell cycle in the somatic cells
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批准号:08102008
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项目类别:Grant-in-Aid for Specially Promoted Research
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资助金额:$197.12万
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财政年份:1996
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负责人:NISHIMOTO Takeharu
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依托单位:
Function of DADl Protein in Programd Cell Death
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批准号:07044203
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项目类别:Grant-in-Aid for international Scientific Research
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资助金额:$1.28万
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财政年份:1995
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负责人:NISHIMOTO Takeharu
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依托单位:
Regulation of MPF activation and Institute of Mitosis
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批准号:05269104
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项目类别:Grant-in-Aid for Scientific Research on Priority Areas
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资助金额:$98.88万
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财政年份:1993
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负责人:NISHIMOTO Takeharu
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依托单位:
cell cycle regulation
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批准号:05269103
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项目类别:Grant-in-Aid for Scientific Research on Priority Areas
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资助金额:$58.82万
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财政年份:1993
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负责人:NISHIMOTO Takeharu
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依托单位:
The function of RCC1/Ran complex.
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批准号:05044134
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项目类别:Grant-in-Aid for international Scientific Research
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资助金额:$1.92万
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财政年份:1993
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负责人:NISHIMOTO Takeharu
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依托单位:
JOINT STUDY ON FUNCTION OF RCC1 PROTEIN IN CELL CYCLE.
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批准号:03044111
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项目类别:Grant-in-Aid for international Scientific Research
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资助金额:$3.84万
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财政年份:1991
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负责人:NISHIMOTO Takeharu
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依托单位:
海外基金