Regulation of MPF activation and Institute of Mitosis

MPF 激活的调节和有丝分裂的研究所

• 批准号: 05269104
• 负责人: NISHIMOTO Takeharu
• 金额: $ 98.88万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05269104/
• 关键词: RCC1; Cyclin A; K-252a; Tom1; Mos; 26S proteasone; MCM; Nda1; ライセンス因子; MOS; TOMI; cdc28; プロテアソーム; 染色体; CDC2; G2; サイクリン; セントロメア; PCC; MPF; G2ブロック

In this group, the cell cycle regulation from G2 to mitosis was investigated from the aspect described as follows.Nishimoto. T : RCC1-Ran cycle. They have cloned RanBP2, RanBP1 from mammalian cells and YRB1, YRB2 and DIS3 from S.cerevisiae, as the Ran-binding protein. Kikuchi, T. : She isolated novel genes, TOM1, STO1 and STO2 required for G2/M transition. Yoshida.M. : He has identified the target gene of K-252, as SRM1. Kobayashi, H. : He has investigated the effect of Xenopus cyclin A overexpression on the cell cycle of S.cerevisiae and found that S.cerevisiae arrested in mitosis without chromosome segregation. Masumoto, H. : Overexpression of CENP-B.Sakata, N. : He has clarified that Mos is a key regulator in the MAP kinase cascade. Tanaka, K. : He has identified proteins comprising the ubiquitin dependent proteasome and cloned the cDNA encoding these proteins. Adachi, Y. : He investigated the relationship between the completion of DNA replication and the initiation of mitosis.

在这一组中，从以下方面研究了从G2到有丝分裂的细胞周期调节。T：RCC 1-运行循环。他们从哺乳动物细胞中克隆了RanBP 2、RanBP 1，从酿酒酵母中克隆了YRB 1、YRB 2和DIS 3，作为Ran结合蛋白。菊池、T.她分离出了G2/M转换所需的新基因TOM 1、STO 1和STO 2。吉田他已经确定了K-252的靶基因，即SRM 1。小林、H.他研究了非洲爪蟾细胞周期蛋白A过表达对酿酒酵母细胞周期的影响，发现酿酒酵母在没有染色体分离的情况下停止有丝分裂。Masumoto，H.：CENP-B的过表达。Sakata，N.：他澄清了Mos是MAP激酶级联反应中的关键调节因子。田中角他已经鉴定出包含泛素依赖性蛋白酶体的蛋白质，并克隆了编码这些蛋白质的cDNA。阿达奇，Y.他研究了DNA复制的完成和有丝分裂的开始之间的关系。

项目成果

N.Yokoyama: "A giant nucleopore protein which binds Ran/TC4." Nature. 376. 184-188 (1995)

N.Yokoyama：“一种结合 Ran/TC4 的巨大核孔蛋白。”

Y.Uesono: "The MCS1/SSD1/SRK1/SSL1 gene is involved in stable maintenance of the chromosome in yeast." Gene. 143. 135-138 (1994)

Y.Ueson：“MCS1/SSD1/SRK1/SSL1 基因参与酵母染色体的稳定维持。”

Nakashima,T.,Nishimoto T.er al.: "Molecular cloning of a human cDNA encoding a novel protein,DAD1,whose defect causes apoptotic cell death in hamster BHK21 cells." Mol.Cell.Biol.13. 6367-6374 (1993)

Nakashima,T.,Nishimoto T.er al.：“编码新型蛋白质 DAD1 的人类 cDNA 的分子克隆，其缺陷导致仓鼠 BHK21 细胞凋亡。”

K. Kitagawa, H. Masumoto et. al.: "Analysis of protein-DNA and protein-protein interactions of centromere proteinB (CENP-B) and properties of the DNA-CENP-B complex in the cell cycle." Mol. Cell. Biol.15. 1602-1612 (1995)

K. Kitakawa，H. Masumoto 等。

M.Dasso,T.Seki,Y.Azuma,T.Ohba and T.Nishimoto: "A mutant form of the Ran/TC4 protein disrupts nuclear functionin Xenopus laevis extracts by inhibiting the RCC1 protein,a regulator of chromosome condensation." EMBO.J.13. 5732-5744 (1994)

M.Dasso、T.Seki、Y.Azuma、T.Ohba 和 T.Nishimoto：“Ran/TC4 蛋白的突变形式通过抑制 RCC1 蛋白（染色体浓缩的调节因子）来破坏非洲爪蟾提取物的核功能。”