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FUNDAMENTAL RESEARCH ON THE ANALYSIS OF POLYCYCLIC AROMATIC HYDROCARBONS IN BLOOD BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY

胶束电动色谱分析血液中多环芳烃的基础研究

基本信息

批准号：
04453040
负责人：
TERABE Shigeru
金额：
$ 4.35万
依托单位：
HIMEJI INSTITUTE OF TECHNOLOGY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

Micellar electrokinetic chromatography (MEKC), which is performed by adding an ionic micelle to the running solution, is a mode of capillary electrophoresis (CE) and capable of separating electrically neutral compounds. CE is a highly efficient an ultramicro analytical technique, and hence it is suitable for the analysis of biological samples. Polycyclic aromatic hydrocarbons (PHAs) are highly hydrophobic and neutral compounds, and therefore they are assumed to be incorporated into the lipoprotein in blood.The present research aimed to optimize the MEKC separation of PAHs and to develop an analytical method of PAHs in lipoproteins. For the separation of PAHs, three techniques were investigated : cyclo-dextrin (CD) modified MEKC (CD-MEKC), where CD is used as a modifier of the partitioning of the analyte ; microemulsion electrokinetic chromatography using a microemulsion instead of the micelle in MEKC ; MEKC using an organic solvent to reduce the distribution of the analyte to the micelle. CD-MEKC using gamma-CD was the most successful in the separation of 16 priority PAHs as pollutants. PAHs in a lipoprotein were successfully analyzed by CD-MEKC using a lipoprotein spiked with a few selected PAHs.The concentration of PAHs in the real blood sample will be much lower than that employed in this research, and therefore high sensitivity detection and/or sample concentration methods are strongly required for analysis of PAHs in blood samples.

胶束电动色谱（MEKC）是毛细管电泳（CE）的一种模式，通过向运行溶液中加入离子胶束来进行，能够分离电中性化合物。毛细管电泳是一种高效的超微量分析技术，适用于生物样品的分析。多环芳烃（Polycyclicaromatic hydrocarbons，PHA）是一种高度疏水的中性化合物，因此被认为与脂蛋白结合在一起，本研究旨在优化MEKC分离多环芳烃的条件，建立脂蛋白中多环芳烃的分析方法。对于多环芳烃的分离，三种技术进行了研究：环糊精（CD）修饰的MEKC（CD-MEKC），其中CD被用作分析物的分配的改性剂;微乳液电动色谱使用微乳液，而不是胶束在MEKC中; MEKC使用有机溶剂，以减少分析物的胶束的分布。使用γ-CD的CD-MEKC是最成功的分离16个优先多环芳烃污染物。采用CD-MEKC法成功地分析了脂蛋白中的多环芳烃，但由于真实的血液样品中多环芳烃的浓度远低于本研究所用的浓度，因此对血液样品中多环芳烃的分析需要高灵敏度的检测和/或样品浓缩方法。