Micellar Electrokinetic Chromatography Using Novel Multi-Chain Surfactants with Two Hydrophilic Groups

使用具有两个亲水基团的新型多链表面活性剂的胶束电动色谱法

• 批准号: 06650934
• 负责人: TANAKA Minoru
• 金额: $ 1.34万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06650934/
• 关键词: Micellar electrokinetic chromatography; Multi-chain surfactants; Naphthalene derivatives; Flavone derivatives

1.Eight multi-chain surfactants having two hydrophilic groups were synthesized. These compounds were bis(sulfonate)-type surfactants derived from diglycidyl ethers of ethylene glycol (1), 1,3-propanediol (2), 2,2-dimethl-1,3-propanediol (3), hydroquinone (4) and N-dodecanoyl-diethanolamine (5), a bis(carboxylate)-type (6) and a bis(sulfate)-type (7) surfactants derived from ethylene glycol diglycidyl ether, and a bis(carboxylate)-type surfactant (8) from 2,2-didodecyl-1,3-propanediol.2.Eight monosubstituted naphthalene derivatives as model analytes could be baseline separated by micellar electrokinetic chromatography (MEKC) using those surfactants as micelle-forming reagents, though 5 could not resolve 1-naphthol and 1-nitronaphthalene. The elution order of the analytes in MEKC with the bis (carboxylate)-type surfactants, 6 and 8 was different from that found with the other surfactants mentioned above. These multi-chain surfactants exhibited the remarkably different selectivities, compared with the elution order with a single-chain surfactant, SDS,used widely in MEKC.3.The MEKC separation of five flavone derivatives, which were not completely resolved with SDS,were also performed. These analytes could be readily baseline separated with 5.0X10^<-3> M4,5 or 6. The elution order of the flavone derivatives with 4,5 and 6 was also different from that with SDS.4.The multi-chain surfactants used here exhibited the remarkably different selectivities of the naphthalene and flavone derivatives in MEKC ; Their separations could be performed with the multi-chain surfactants at considerably lower concentrations, compared with single-chain SDS.

1.合成了八个具有两个亲水性基团的多链表面活性剂。这些化合物是由乙二醇二甘油酯(1)、1,3-丙二醇(2)、2,2-二甲基-1,3-丙二醇(3)、对苯二酚(4)和n -十二烷基二乙醇胺(5)的二甘油酯型(6)和二（硫酸盐）型(7)表面活性剂，由2,2-二十二烷基-1,3-丙二醇2衍生的二（羧酸）型表面活性剂(8)。8种单取代萘衍生物作为模型分析物，可以用胶束电动色谱法（MEKC）将其作为胶束形成试剂进行基线分离，但5种不能分离1-萘酚和1-硝基萘。羧酸双酯型表面活性剂6和8在MEKC中的洗脱顺序与其他表面活性剂不同。与MEKC.3中广泛使用的单链表面活性剂SDS的洗脱顺序相比，这些多链表面活性剂表现出明显不同的选择性。对5种黄酮类化合物进行了MEKC分离，SDS无法完全分离。这些分析物可以很容易地基线分离5.0 × 10^<-3> M4,5或6。4、5、6黄酮衍生物的洗脱顺序也与SDS.4不同。多链表面活性剂对MEKC中萘和黄酮衍生物的选择性有显著差异；与单链SDS相比，多链表面活性剂可以在较低的浓度下对其进行分离。

项目成果

Harino, Hiroya: "Double-Chain Surfactants with Two Sulfonate Groups as Micelle-Forming Reagents in Micellar Electrokinetic Chromatography of Naphthalene Derivatives" Journal of Chromatography A. 715. 135-141 (1995)

Harino, Hiroya：“具有两个磺酸盐基团的双链表面活性剂作为萘衍生物胶束电动色谱中的胶束形成试剂”《色谱杂志》A. 715. 135-141 (1995)

Harino, Hiroya: "Separation of Naphthalene and Flavone Derivatives by Micellar Electrokinetic Chromatography with Double- and Triple-Chain Surfactants" Journal of Chromatography A. (in contribution).

Harino, Hiroya：“使用双链和三链表面活性剂通过胶束电动色谱法分离萘和黄酮衍生物”《色谱杂志》A.（投稿中）。