DEVELOPMENT OF HIGHLY SENSITIVE AND RAPID SEPARATION-DETECTION SYSTEM FOR ENVIRONMENTAL POLLUTANTS BY CAPILLARY ELECTROPHORESIS

高灵敏快速毛细管电泳环境污染物分离检测系统的研制

• 批准号: 11793016
• 负责人: TERABE Shigeru
• 金额: $ 2.82万
• 依托单位: HIMEJI INSTITUTE OF TECHNOLOGY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for University and Society Collaboration
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11793016/
• 关键词: Capillary Electrophoresis; Micellar Electrokinetic Chromatography; Microchip Electrophoresis; On-line Sample Concentration; Mass Spectrometry; Atmospheric Pressure Chemical Ionization; Environmental Science; Environmental Pollutants; 環境分析; マイクロチッ

The aim of this project was to develop a highly sensitive and rapid analysis system for environmental pollutants by using capillary electrophoresis (CE), including micellar electrokinetic chromatography (MEKC) using on-line sample concentration techniques for enhancing the detection sensitivity and CE using mass spectrometry (MS) as a high performance detection scheme.1. Optimization of separation and detection conditions in an on-line sample concentration-MEKC system was carried out for several model compounds, such as dioxins, dialkyl phthalates, aromatic amines, and chiral pesticide components. As a result, the ten to thousands-fold increase in sensitivity was attained compared to conventional MEKC system.2. Combination of sweeping, an on-line sample concentration method, with MEKC-MS employing an atmospheric pressure chemical ionization (APCI) interface was investigated. Several hundreds-fold increase in detection sensitivity was obtained for dialkyl phthalates and organic amines compared with conventional MEKC-APCI-MS system.3. Fundamental studies on MEKC using synthesized ionic high molecular-mass surfactants (HMMSs) were carried out. Several polycyclic aromatic hydrocarbons were successfully separated with an HMMS solution containing large amount of organic modifiers.4. The change in peak profile during sweeping was successfully observed by using microchip electrophoresis (MCE) and the mechanism of sweeping was interpreted.5. A study on on-line combination of MCE with an MS detection (MCE-MS) was carried out using electrospray ionization as an interface. The use of a short capillary attached to the edge of the microchip as a spray nozzle was found to be effective for a stable ESI.

该项目的目的是利用毛细管电泳（CE）开发一种高灵敏度、快速的环境污染物分析系统，包括使用在线样品浓缩技术提高检测灵敏度的胶束电动色谱（MEKC）和使用质谱（MS）作为高性能检测方案的CE。1。对二恶英、邻苯二甲酸二烷基酯、芳香胺和手性农药成分等多种模型化合物的在线样品浓缩-MEKC系统中的分离和检测条件进行了优化。结果，与传统MEKC系统相比，灵敏度提高了十到数千倍。2．研究了在线样品浓缩方法——扫描与采用大气压化学电离 (APCI) 接口的 MEKC-MS 的结合。与传统的MEKC-APCI-MS系统相比，邻苯二甲酸二烷基酯和有机胺的检测灵敏度提高了数百倍。 3．使用合成离子高分子表面活性剂（HMMS）对MEKC进行了基础研究。利用含有大量有机改性剂的HMMS溶液成功分离了多种多环芳烃。 4．利用微芯片电泳(MCE)成功地观察到了扫描过程中峰形的变化，并解释了扫描的机理。 5．使用电喷雾电离作为接口，进行了 MCE 与 MS 检测（MCE-MS）在线组合的研究。人们发现，使用连接到微芯片边缘的短毛细管作为喷嘴对于稳定 ESI 是有效的。

项目成果

Koji Otsuka: "Electrophoretic Analysis Using Microchips"Electrochemistry. 69. 624-629 (2001)

Koji Otsuka：“使用微芯片进行电泳分析”电化学。

Philip Britz-McKibbin: "On-line Focusing of Flavin Derivatives Using Dynamic pH Junction-Sweeping Capillary Electrophoresis with Laser-Induced Fluorescence Detection"Anal. Chem.. 74. 3736-3743 (2002)

Philip Britz-McKibbin：“使用动态 pH 结点扫描毛细管电泳和激光诱导荧光检测在线聚焦黄素衍生物”。

Yoichi Sera: "Sweeping on a Microchip: Concentration Profiles of the Focused Zone in Micellar Electrokinetic Chromatography"Electrophoresis. 22. 3509-3513 (2001)

Yoichi Sera：“扫描微芯片：胶束电动色谱中聚焦区的浓度分布”电泳。

Jong-Bok Kim: "On-line Sample Concentration in MEKC Using Cationic Surfactants"Journal of Chromatography A. (in press). (2001)

Jong-Bok Kim：“使用阳离子表面活性剂在 MEKC 中进行在线样品浓缩”Journal of Chromatography A.（出版中）。

Koji Otsuka: "Enantiomer Separation of Drugs by Micellar Electrokinetic Chromatography Using Chiral Surfactants"Journal of Chromatography A. 875. 163-178 (2000)

Koji Otsuka：“使用手性表面活性剂通过胶束电动色谱法分离药物的对映体”色谱杂志 A. 875. 163-178 (2000)