Study on the post-transcriptional regulators of cyanobacteria and chloroplasts

蓝藻和叶绿体转录后调控因子的研究

• 批准号: 04454003
• 负责人: SUGITA Mamoru
• 金额: $ 4.03万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454003/
• 关键词: RNA-binding protein; RNA-binding domain; Cyanobacteria; Chloroplast; Post-transcripnal regulation; Gene; タバコ; シロイヌナズナ; アミノ酸配列

1.The unicellular cyanobacterium, Synechococcus sp. PCC 6301 has two proteins containing an RNA-binding domain homologous to the chloroplast counterparts. This is the first evidence of RNA-binding proteins (RNPs) in prokaryotes and confirms that chloroplast RNPs are of cyanobacterial origin.2.Analysis of the genes encoding chloroplast RNPs from tobacco and Arabidopsis thaliana indicated that chloroplast RNPs can be categorized into three groups.3.Chloroplast RNPs were demonstrated to be associated with a variety of chloroplast mRNA species, non-ribosomal 16S rRNA and intron-containing pre-tRNAs as stable RNA-protein complexes. This observation strongly suggests that the chloroplast RNPs are involved in RNA processing and RNA stability.4.We discovered novel types of RNA-binding proteins. These novel proteins contain an N-terminal RNA-binding domain and C-terminal glycine-rich domain, therefore they are named RGP (RNA-binding Glycine-rich Proteins). RGP proteins are possibly involved in post-transcriptional regulation in nucleus.5.Based on the RNA-binding domain structures and nucleic acids-binding properties, we discussed evolutionary relationship of the above three types of RNA-binding proteins.

1.单细胞蓝细菌，聚球藻属PCC 6301具有两种蛋白质，其含有与叶绿体对应物同源的RNA结合结构域。2.对烟草和拟南芥叶绿体RNA结合蛋白基因的分析表明，叶绿体RNA结合蛋白可分为三大类：3.叶绿体RNA结合蛋白与多种叶绿体mRNA种类相关，非核糖体16 S rRNA和含内含子的前体tRNA作为稳定的RNA-蛋白质复合物。这一发现有力地说明叶绿体RNP参与了RNA的加工和稳定。4.我们发现了新的RNA结合蛋白。这些新型蛋白质含有N末端RNA结合结构域和C末端富含甘氨酸结构域，因此被命名为RGP（RNA结合富含甘氨酸蛋白质）。RGP蛋白可能在细胞核内参与转录后调控。5.根据RNA结合结构域的结构和核酸结合特性，探讨了上述三种RNA结合蛋白的进化关系。

项目成果

杉田 護: "植物遺伝子の転写後制御機構ー葉緑体RNA結合蛋白質ー" 蛋白質 核酸 酵素. 37. 1155-1164 (1992)

Mamoru Sugita：“植物基因的转录后控制机制——叶绿体RNA结合蛋白”蛋白质核酸酶37。1155-1164（1992）

太田 賢: "The structure and expression of chloroplast ribonucleo-protein genes from Arabidopsis thaliana. in “Research in Photosynthesis"" Kluwer Academic Press, (1992)

Ken Ota：“拟南芥叶绿体核糖核蛋白基因的结构和表达”，《光合作用研究》，Kluwer 学术出版社，(1992)

板谷 啓: "Structure and expression of a cDNA encoding an RNA helicase-like protein in tobacco." Plant Molecular Biology. 24. 249-252 (1994)

Kei Itaya：“烟草中编码 RNA 解旋酶样蛋白的 cDNA 的结构和表达。” 24. 249-252 (1994)。

杉田 護: "Sttucture and expression of the gene encoding ribosomal protein SI from the cyanobacterium Synechococcus sp.strain PCC6301:striking sequence similarity to the chloroplast ribosomal protein CSI." Molecular and General Genetics. 246. 142-147 (1995)

Mamoru Sugita：“蓝藻聚球藻菌株 PCC6301 中编码核糖体蛋白 SI 的基因的结构和表达：与叶绿体核糖体蛋白 CSI 的惊人序列相似性。分子和普通遗传学”。

Sugita, Mamoru: "Structure and expression of the gene encoding ribosomal protein S1 from the cyanobacterium Synechococcus sp. strain PCC 6301 : striking sequence similarity to the chloroplast ribosomal protein CS1." Mol.Gen.Genet.246. 142-147 (1995)

Sugita, Mamoru：“蓝藻聚球藻菌株 PCC 6301 中编码核糖体蛋白 S1 的基因的结构和表达：与叶绿体核糖体蛋白 CS1 具有惊人的序列相似性。”