Dynamism of plastid gene expression by intracellular communication

细胞内通讯的质体基因表达动态

• 批准号: 14340252
• 负责人: SUGITA Mamoru
• 金额: $ 10.5万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14340252/
• 关键词: Chloroplast; Physcommitrella patens; Plastid transformation; Relic gene; arginine tRNA gene; Plastid RNA polymerase; plastid sigma factor; PPR protein; RNA編集; タバコ; DNAチップ; 概日リズム; Lhcb遺伝子; ミトコンドリア; 翻訳開始コドン; シグマ因子; ファージ型RNAポリメラーゼ

This research purpose is to define the network of gene expression by communication between organelles and nuclear genomes within a plant cell. The novel findings obtained are as follows.1.We constructed a plastid DNA chip for microarray analysis of plastid genes from the moss, Physcomitrella patens.2.We reported phylogenetic analyses using 51 genes from the entire plastid genome sequences of 20 representative plant species. This indicated that extant bryophytes (mosses, liverworts, and hornworts) form a monophyletic group with high statistical confidence and that extant bryophytes are likely sisters to extant vascular plants. We propose bryophyte monophyly as the current best hypothesis.3.We developed plastid transformation technique for the moss, Physcomitrella patens and constructed stable plastid trnR-CCG knockout moss transformants. The trnR-CCG knockout transformants indicated that the P.patens trnR-CCG gene is not essential for plastid function.4.We identified a nuclear gene, PpR … More poA, encoding the alpha subunit of plastid-encoded plastid RNA polymerase (PEP). We generated and characterized PpRpoA knockout mosses. Primer extension, northern blot, run-on transcription assay and in vitro transcription assays first demonstrated that both PEP and NEP(nuclear-encoded plastid RNA polymerase) exist in the moss and recognize the common promoters of photosynthesis genes and non-photosynthesis genes.5.We identified three PpSig genes encoding plastid sigma factor. Among the three PpSig genes, only PpSig5 was clearly controlled by the circadian clock and/or by blue light signaling in the moss P.patens.6.An extensive survey of the Physcomitrella expressed sequence tag(EST) databases revealed 36 ESTs encoding pentatricopeptide repeat(PPR) motif-containing proteins. We further characterized five full-length cDNAs encoding plastid-localized PPR proteins, PPR513-10 and PPR566-6 were expressed differentially in protonemata grown under different light-dark conditions, suggesting they have distinctive functions in chloroplasts. This is the first report and analysis of genes encoding PPR proteins in bryophytes. Less

本研究的目的是确定植物细胞内细胞器和核基因组之间的基因表达网络。本研究获得了以下新的发现：1.构建了一种用于藓类植物小立碗藓（Physcomitrella patents）质体基因芯片; 2.对20种代表性植物的51个基因进行了系统发育分析。这表明，现存的苔藓植物（苔藓，地钱，金鱼藻）形成一个单系组具有较高的统计置信度，现存的苔藓植物可能是姐妹现存的维管植物。我们提出苔藓植物单系性是目前最好的假设。3.我们开发了苔藓立碗藓的质体转化技术，构建了稳定的质体trnR-CCG基因敲除苔藓转化子。trnR-CCG基因敲除的转化子表明，小展枝青霉trnR-CCG基因不是质体功能所必需的 ...更多信息 poA，编码质体编码的质体RNA聚合酶（PEP）的α亚基。我们产生并表征了PpRpoA敲除苔藓。引物延伸、北方杂交、转录检测和体外转录检测等方法首次证实了PEP和NEP（nuclear-encoded plastid RNA polymerase）在苔藓中的存在，并识别光合作用基因和非光合作用基因的共同启动子。在这3个PpSig基因中，只有PpSig 5基因在藓类植物中受到生物钟和/或蓝光信号的明显控制。6.对立碗藓表达序列标签（EST）数据库的广泛调查显示，有36个EST编码含五肽重复（PPR）基序的蛋白。我们进一步表征了5个编码质体定位的PPR蛋白的全长cDNA，PPR 513 -10和PPR 566 -6在不同光暗条件下生长的原丝体中差异表达，表明它们在叶绿体中具有独特的功能。这是首次报道和分析的PPR蛋白编码基因的brabrites。少

项目成果

Nakamura, T., Furuhashi, Y., Hasegawa, K., Hashimoto, H., Obokata, J., Sugita, M., Sugiua, M.: "Array-based analysis on tobacco plastid transcripts : preparation of a genomic microarray containing all genes and intergenic regions"Plant Cell Physiol. 44. 8

Nakamura, T.、Furuhashi, Y.、Hasekawa, K.、Hashimoto, H.、Obokata, J.、Sugita, M.、Sugiua, M.：“基于阵列的烟草质体转录物分析：基因组微阵列的制备

Chloroplast phylogeny indicates that bryophytes are monophyletic.

• DOI: 10.1093/molbev/msh203
• 发表时间: 2004-10
• 期刊: Molecular biology and evolution
• 影响因子: 10.7
• 作者: T. Nishiyama;P. Wolf;M. Kugita;R. Sinclair;M. Sugita;C. Sugiura;T. Wakasugi;Kyoji Yamada;K. Yoshinaga;K. Yamaguchi;K. Ueda;M. Hasebe

Sugiura, C., Kobayashi, Y., Aoki, T., Sugita, C., Sugita, M.: "Complete choloroplast DNA sequence of the moss Physcomitrella patens reveals loss of rpoA from the chloroplast genome : evidence for the loss and relocation of rpoA from the chloroplast to the

Sugiura, C.、Kobayashi, Y.、Aoki, T.、Sugita, C.、Sugita, M.：“苔藓小立碗藓的完整叶绿体 DNA 序列揭示了叶绿体基因组中 rpoA 的丢失：丢失和重新定位的证据

Tanaka, M., Ogawa, N., Ihara, K., Sugiyama, S., Mukohata, Y.: "Cytochrome aa3 and its gene duplication in Haloferax volcanii"J. Bacteriol.. 184. 840-845 (2002)

Tanaka, M.、Okawa, N.、Ihara, K.、Sugiyama, S.、Mukohata, Y.：“Haloferax volcanii 中的细胞色素 aa3 及其基因复制”J.

Yamaguchi, K., Mayfield, S.P., Sugita, M.: "Transcriptional and translational regulation in biosynthesis and repair. In : Photosystem II : The water/plastoquinone oxido-reductase in photosynthesis"Kluwer Publications (in press). (2003)

Yamaguchi, K.、Mayfield, S.P.、Sugita, M.：“生物合成和修复中的转录和翻译调节。见：光系统 II：光合作用中的水/质体醌氧化还原酶”Kluwer Publications（正在出版）。