RNA BINDING DOMAIN OF RHO
RNA BINDING DOMAIN OF RHO
批准号:
2750142
负责人:
GORDON S. RULE
金额:
$16.17万
依托单位国家:
美国
项目类别:
财政年份:
1996
资助国家:
美国
项目状态:
已结题
起止时间:
1996-08-01 至 1999-07-31
中文摘要
Rho是释放新生RNA转录物所需的蛋白质
英文摘要
Rho is a protein required for the release of nascent RNA transcripts from
a large number of transcription complexes in both gram-negative and
gram-positive bacteria. In vitro, Rho binds single stranded DNA and RNA,
but preferential activation of helicase activity by cytidine-containing
oligoribonucleotides leads to termination of transcription. Since Rho
protein plays a central role in the regulation of protein synthesis it
is a significant and essential component of bacterial metabolism.
Information about Rho-nucleic acid interactions at the atomic level may
be of potential aid in the development of novel anti-bacterial agents.
Within the past decade the nucleic acid binding properties, enzymology,
and regulatory properties of this important protein have been the subject
of intense studies by a number of investigators. Additionally, a large
number of mutations of this protein have been made in an effort to
understand the mechanism of Rho. Although this work has lead to
significant advances in our understanding of Rho function, these advances
have not been able to address the mechanism at the atomic level because
there is no information on the structure of Rho at this level.
The proposed research will investigate the structure of the RNA binding
domain of Rho, a 15kDa fragment which has been expressed in E. coli. Both
the NMR solution structure and the x-ray crystallographic structure of
this domain will be determined in the presence and absence of various RNA
and DNA oligonucleotides. The site size and binding constant for the
binding of DNA and RNA oligonucleotides to this domain of Rho will be
evaluated. Analysis of this binding data, in conjunction with the atomic
structure of the RNA binding domain, will provide a new perspective in
the understanding of Rho function. Further, since Rho contains a novel
RNA-binding motif, these studies will also greatly enhance knowledge of
RNA-protein interactions in general.
期刊论文(1)
专著(0)
科研奖励(0)
会议论文
Effect of deuteration on the accuracy of HN-HN distance constraints.
氘化对 HN-HN 距离约束精度的影响。
DOI:
10.1006/jmre.1998.1511
发表时间:
1998
期刊:
Journal of magnetic resonance (San Diego, Calif. : 1997)
影响因子:
--
作者:
[Briercheck,DM, Rule,GS]
通讯作者:
Rule,GS
Discovery of Thymidylate Kinase Inhibitors for Anti-Fungal Applications
-
批准号:10553160
-
项目类别:
-
资助金额:$23.53万
-
财政年份:2022
-
负责人:GORDON S. RULE
-
依托单位:
Discovery of Thymidylate Kinase Inhibitors for Anti-Fungal Applications
-
批准号:10453065
-
项目类别:
-
资助金额:$19.6万
-
财政年份:2022
-
负责人:GORDON S. RULE
-
依托单位:
Cryoprobe for 600 MHz Biomolecular NMR
-
批准号:6578394
-
项目类别:
-
资助金额:$23.93万
-
财政年份:2003
-
负责人:GORDON S. RULE
-
依托单位:
DYNAMICS OF GLUTATHIONE TRANSFERASES
-
批准号:6525524
-
项目类别:
-
资助金额:$22.17万
-
财政年份:2001
-
负责人:GORDON S. RULE
-
依托单位:
DYNAMICS OF GLUTATHIONE TRANSFERASES
-
批准号:6618100
-
项目类别:
-
资助金额:$22.17万
-
财政年份:2001
-
负责人:GORDON S. RULE
-
依托单位:
DYNAMICS OF GLUTATHIONE TRANSFERASES
-
批准号:6786677
-
项目类别:
-
资助金额:$21.74万
-
财政年份:2001
-
负责人:GORDON S. RULE
-
依托单位:
DYNAMICS OF GLUTATHIONE TRANSFERASES
-
批准号:6384060
-
项目类别:
-
资助金额:$21.95万
-
财政年份:2001
-
负责人:GORDON S. RULE
-
依托单位:
Expression and Characterization of Torsin A
-
批准号:6361757
-
项目类别:
-
资助金额:$10.82万
-
财政年份:2001
-
负责人:GORDON S. RULE
-
依托单位:
Expression and Characterization of Torsin A
-
批准号:6530043
-
项目类别:
-
资助金额:$11.09万
-
财政年份:2001
-
负责人:GORDON S. RULE
-
依托单位:
RNA BINDING DOMAIN OF RHO
-
批准号:2194318
-
项目类别:
-
资助金额:$14.0万
-
财政年份:1996
-
负责人:GORDON S. RULE
-
依托单位:
RNA BINDING DOMAIN OF RHO
-
批准号:2459733
-
项目类别:
-
资助金额:$16.82万
-
财政年份:1996
-
负责人:GORDON S. RULE
-
依托单位:
SUBSTRATE SPECIFICITY OF GLUTATHIONE TRANSFERASES
-
批准号:2417144
-
项目类别:
-
资助金额:$11.09万
-
财政年份:1994
-
负责人:GORDON S. RULE
-
依托单位:
SUBSTRATE SPECIFICITY OF GLUTATHIONE TRANSFERASES
-
批准号:2022515
-
项目类别:
-
资助金额:$5.81万
-
财政年份:1994
-
负责人:GORDON S. RULE
-
依托单位:
SUBSTRATE SPECIFICITY OF GLUTATHIONE TRANSFERASES
-
批准号:2184209
-
项目类别:
-
资助金额:$4.9万
-
财政年份:1994
-
负责人:GORDON S. RULE
-
依托单位:
SUBSTRATE SPECIFICITY OF GLUTATHIONE TRANSFERASES
-
批准号:2184208
-
项目类别:
-
资助金额:$9.99万
-
财政年份:1994
-
负责人:GORDON S. RULE
-
依托单位:
SUBSTRATE SPECIFICITY OF GLUTATHIONE TRANSFERASES
-
批准号:2634697
-
项目类别:
-
资助金额:$11.73万
-
财政年份:1994
-
负责人:GORDON S. RULE
-
依托单位:
SUBSTRATE SPECIFICITY OF GLUTATHIONE TRANSFERASES
-
批准号:2184207
-
项目类别:
-
资助金额:$8.38万
-
财政年份:1994
-
负责人:GORDON S. RULE
-
依托单位:
STRUCTURE OF PHOSPHOLIPASE A2
-
批准号:3869060
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:GORDON S. RULE
-
依托单位:
NMR STUDIES OF PROTEIN STRUCTURE
-
批准号:3890400
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:GORDON S. RULE
-
依托单位:
NMR STUDIES OF PROTEIN STRUCTURE
-
批准号:3869041
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:GORDON S. RULE
-
依托单位:
海外基金