权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Autonomous ditributed control system in the autonomic nervous system : Physiological and bio-engineering approaches

自主神经系统的自主分布式控制系统：生理学和生物工程方法

基本信息

批准号：
04454035
负责人：
KATAYAMA Yoshifumi
金额：
$ 4.16万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

项目摘要

1.The small intestine isolated from adult guinea-pigs provoked perisitaltic movement. The ability of provoking peristalsis was preserved in the intestine from which both mucosa and submucosa were mechainically removed but which contained the myenteric plexus and two smooth muscle layrs (circular and longuitudinal) . It is speculated that the peristalsis is regulated by mechanisms organized within the enteric nervous system independent from the central nervous system. Thus, the intestine is provided with an intrinsic autonomic control system with regards to the intestinal movement.2.Actions of neuropeptides, neuropeptide Y,substance P and neurokinin A,in the enteric nervous system were analyzed using various portions of the intestine, demonstrating the evidence of regional differences in the function of the enteric neurons. ATP and L-DOPA modulated transmitter release in the enteric nervous system.3.A large amount of Ca influx activated Ca-binding of Ca-bindeing proteins, Ca extrusion to extracellular space, Ca uptake into Ca storage sites, and Ca pump. All of these are involved in maintaining the cellular Ca homeostasis. Na-K pump alpha3 isoform was found to localize both in nerve cells and in the heart excitation conducting system, suggesting the alpha3 isoform pump function is essential to the autonomous excitation of excitable cells.4.Growth cones of cultured neurones isolated from the diagonal band of Broca of new born rats were observed using the video-enhanced differential interference contrast microscopy. Filopodia rapidly elongated at about 1mum/s from the growth cone in response to high potassium stimulation and retracted upon the end of stimulation. The tip of folopodia moved apparently in a Brownian manner. Before making a contact with other neighboring neurons, a transmitter substance, acetylcholin, was stored and even released in and from the growth cone. These processes are supposed to play an important role in organizing neural networks.

1.分离的成年豚鼠小肠可引起坐骨神经运动。引起蠕动的能力保留在肠中，机械地去除了粘膜和粘膜下层，但含有肌间神经丛和两层平滑肌层(环状和纵向)。据推测，肠蠕动是由肠神经系统内独立于中枢神经系统的机制调节的。2.利用肠道的不同部位分析了神经肽Y、P物质和神经激肽A在肠道神经系统中的作用，证明了肠道神经元功能的区域差异。3.大量的钙内流激活了钙结合蛋白的钙结合、钙向胞外空间的排泄、钙向钙储存部位的摄取和钙泵。所有这些都与维持细胞内钙稳态有关。Na-K泵α3亚型定位于神经细胞和心脏兴奋传导系统，提示α3亚型泵功能对兴奋性细胞的自主兴奋是必不可少的。4.用视频增强差示干涉显微镜观察新生大鼠Broca斜角带分离培养神经元的生长锥体。丝状伪足在高钾刺激下以约1微米/S的速度从生长锥上迅速伸长，刺激结束后收缩。叶柄顶端呈明显的棕色运动。在与其他邻近神经元接触之前，一种递质物质乙酰胆碱被储存在生长锥内，甚至从生长锥中释放出来。这些过程被认为在组织神经网络方面发挥着重要作用。