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視蓋吻尾軸の決定機構:その遺伝子的背景と神経回路形成

顶盖头尾轴的决定机制：遗传背景和神经回路形成

基本信息

批准号：
04454130
负责人：
仲村春和
金额：
$ 3.84万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1993
项目状态：
已结题

项目摘要

ニワトリ視蓋原基では〓卵2日目よりホメオボックス遺伝子の一つengrailed(en)が尾側に強く,吻側に弱いという勾配をもって発現される。我々の一連の研究により,enの発現がその後の視蓋の極性を決めるのに関係していることが示唆されている.〓卵して2日目胚の中脳(視蓋原基)を間脳部に移植すると,移植片は異所的視蓋として分化し,enの発現パターンはもともとの運命とは逆転した。ホストを3日目胚,移植片を2日目中脳とすると,enの発現パターンの逆転はおこらず,もともとの発現パターンを示した。いずれの場合も,移植片でのenの発現パターンと網膜視蓋投射パターンは強い相関を示した。これまでの結果によると視蓋の吻尾軸は〓卵3日目に決定されるが,本研究結果ともよく一致する.en遺伝子の役割を直接証明するつもりで,5年度にはenの強制発現のシステムを確立した。ラウスサルコーマウイルスに由来する強制発現ベクターをHughes博士より譲り受け,それにen-2のcDNAを組みこんだ。このレトロウイルスは自己増殖可能なウイルスである。このウイルスを,ウイルス感受性胚の培養線維芽細胞で増殖させ,〓卵2〜2.5日のウイルス感受性胚の中脳付近の神経管中に注入したところ,注入部付近で高濃度にenを発現させることができた。検出はEn-2蛋白を認識するモノクローン抗(本4D9を用いて行った。次にウイルス感受性胚に感染させ,その視蓋原基をウイルス抵抗性胚に移植すると,移植した視蓋原基でのみenの強制発現が見られた。このように目的とする部域での限局した発現のシステムが確立されたので,現在このような視蓋への網膜線維の投射パターンの解析を行っている。

In the second day of the experiment, the primordium has a strong tail (en), a weak match, and a weak match. We linked to the study, and en said that in the future, there was no significant difference between the two parts of the embryo (primordium) in the transplantation of the graft. in the second day of the embryo, the transplantation was carried out in the middle of the embryo (primordium), and the graft was grafted in the middle of the embryo (primordium). The grafts were not differentiated, and the en images showed that there was an adverse reaction in terms of life. Three-day embryo, transplant film in the second day of the eye, the en will see that there is an adverse effect on the embryo, and that the film will show that the embryo is in the opposite direction. When the image is closed, the graft en will show that the network network is projected to show the strength of each other. The results of this study showed that the results of this study were consistent with each other. The results of this study were consistent with the results of this study. The results of this study showed that the results of this study were consistent with each other. The results of this study showed that the results of this study were consistent. In this study, the results of this study showed that the results of this study were consistent. In this study, the results of this study showed that the results of this study were consistent. In this study, the results of this study showed that the results of this study were consistent. In this study, the results of this study were consistent with the results of this study. In this study, the results of this study showed that the results of this study were consistent. In this study, the results of this study were consistent. In this study, the results of this study were consistent. In this study, the results of this study were consistent. The reason for this is that it is necessary to see that Dr. Hughes is suffering from the disease, and that en-2 cDNA is responsible for this. If you don't want to colonize yourself, you may have to do so. After 2 ~ 2.5 days of incubation, the receptive embryos were injected into the divine tube, and the injection part was close to the high concentration of en. The expression of En-2 protein is known to be sensitive to anti-En-2 protein. (this 4D9 is used in this document. The susceptible embryos were infected, the primordia were transferred into resistant embryos, and the primordia were transferred into en cells. In order to achieve this goal, the local office will not be able to make sure that the system has been set up, and now the network network will be able to project the data to parse the bank account.