THE SYUDY OF SIGNAL TRANSDUCTION IN DISEASED HEART AND MOLECULAR CLONING OF NEW G PROTEIN.

病变心脏信号转导的研究和新G蛋白的分子克隆。

• 批准号: 04454259
• 负责人: KAWAGUCHI Hideki
• 金额: $ 4.35万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454259/
• 关键词: GTP BINDING PROTEIN; HYPERTROPHIC CARDIOMYOPATHY; DCM; SIGNAL TRANSDUCTION; alpha ADRENOCEPTOR; beta ADRENOCEPTOR; G蛋白質; 心筋症; 心筋症モデル動物; PLC; 1P3

Free beta gamma subunits of GTP binding protein can themselves interact functionally with protein. Recent results suggest that beta subunit might be playing a key role to specify the receptor-effector coupling in various transmembrane signaling systems. The purpose of this study is to examine the expression level and distribution of G protein beta subunit in rat hearts.Brain and ventricle of WKY rats were used to examined the expression of three different isoforms of beta subunits in mRNA and protein level by means of Northern bolt and Western bolt analysis, respectively.beta3 mRNA was expressed significantly more in ventricles than in brains. This result was supported by Western bolt analysis showing that beta3 was expressed more in ventricles. For all three isoforms, the majority of beta subunits expressed in rats hearts were detected in cytosolic fraction. An ultrafiltration and susequent Western bolt analysis revealed that the beta subunits in ventricular cytosolic fractions had molecular mass greater than 100,000 dalton.In this study, tissue-specific expression of beta subunits isoforms were revealed in rat hearts.

GTP结合蛋白的游离β - γ亚基本身可以与蛋白质发生功能相互作用。最近的研究结果表明β亚基可能在各种跨膜信号系统中发挥关键作用，以指定受体-效应器耦合。本研究的目的是检测G蛋白β亚基在大鼠心脏中的表达水平和分布。采用Northern bolt法和Western bolt法分别检测WKY大鼠脑和脑室三种不同亚型β亚基mRNA和蛋白水平的表达。β a3 mRNA在脑室中的表达明显高于在脑中的表达。Western bolt分析证实，β a3在心室中表达较多。对于所有三种亚型，在大鼠心脏中表达的大多数β亚基都在细胞质部分检测到。超滤和随后的Western bolt分析显示，心室细胞质组分中的β亚基分子质量大于100,000道尔顿。在这项研究中，揭示了β亚基亚型在大鼠心脏中的组织特异性表达。

项目成果

Hideaki Kawaguchi: "Phosphatidylinositol Metabolism in hypertrophic rat heart" Cin Res. 72. 966-972 (1993)

Hideaki Kawaguchi：“肥厚大鼠心脏中的磷脂酰肌醇代谢”Cin Res。

Hideaki Kawaguchi: "Increased calcium release from sarcoplasmic reticulum stimulated by inositol trisphosphate in spontaneously hypertensive rat heart cells" Mol Cell Biochem. 119. 51-57 (1993)

Hideaki Kawaguchi：“自发性高血压大鼠心脏细胞中肌醇三磷酸刺激肌浆网钙释放增加”Mol Cell Biochem。

Wataru Sato: "Deletion of mitochondrial DNA in a patient with condaction block" Am heart J. 125. 550-552 (1993)

Wataru Sato：“传导阻滞患者线粒体 DNA 的删除”Am heart J. 125. 550-552 (1993)

Hiroshi Okamoto: "Molecular dynamics of the phospholipid bilayr in cardiomyopathic hamster heart cell membrane" J Mol Cell Cardiol. (in press).

Hiroshi Okamoto：“心肌病仓鼠心脏细胞膜中磷脂双层的分子动力学”J Mol Cell Cardiol。

Mikako Shoki: "Phosphatidylinositol metabolism in hypertrophic rat heart" Jap.Circ.J.56. 142-147 (1992)

Mikako Shoki：“肥厚大鼠心脏中的磷脂酰肌醇代谢”Jap.Circ.J.56。