Selective destruction of in vivo bacterial wall with laser (Especially for osteomyelitis and arthritis)

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The purpose of this research is to estabilish Laser Therapy for purulent disorders due to MRSA infection. And this study was initiated to seek a photochemical bactericidal therapy encompassing thermal effects of laser irradiation combined with dyes. At first, preliminary experiments were done to know basic informations of dyes and MRSA.1. Spectrophotometrtic determination of dye solutions. 2. Bacterial growth-inhibitory effect and bacterial staining. 3. Bacterial thermosensitivity. 4. Exothermic dye reaction to laser reaction. 5. After 1-4 experimental studies, the study wsa explore the effects of laser irradiation on the organisms, alone and in combination with prior treatment with dyes. The growth of MRSA was inhibited by argon laser wiht or without dyes (MB,PSP,ICG). And zone of growth inhibition treated with dye became distinct. Further attempts were done to know the mechanism of bacterial destruction by argon laser irradiation. And following results were comfirmed : 1. Not only MRSA,but MSSA,E.Cloli and P.S.were destructed by the argon laserirradiation. Moreover, P.S.was destructed with a half energy of laser irradiation. 2. The destruction of bacteria with laser depended on the total Joules, not on peak power. 3. From TEM observation, cytoplasma an DNA oflased MRSA was changed the structure and density. But bacteriolysys was not occurred. These changes were a little similar to the image of heat damaged MRSA compared with that of UV damaged MRSA.4. From stained method with acrigine-orange whitch is able to stain DNA and RNA,and electrophoresis, the structure of DNA was not destructed. From our results, we guess that initial trigger point of laser destruction exists in cytoplasma.