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Selective destruction of in vivo bacterial wall with laser (Especially for osteomyelitis and arthritis)

用激光选择性破坏体内细菌壁（特别是对于骨髓炎和关节炎）

基本信息

批准号：
04454371
负责人：
ABE Yusuke
金额：
$ 4.74万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454371/
关键词：
Argon Laser MRSA Dye Sterilization DNA Cytoplasm レーザー黄色ブドウ球菌可視光近赤外線体内感染巣

项目摘要

The purpose of this research is to estabilish Laser Therapy for purulent disorders due to MRSA infection. And this study was initiated to seek a photochemical bactericidal therapy encompassing thermal effects of laser irradiation combined with dyes. At first, preliminary experiments were done to know basic informations of dyes and MRSA.1. Spectrophotometrtic determination of dye solutions. 2. Bacterial growth-inhibitory effect and bacterial staining. 3. Bacterial thermosensitivity. 4. Exothermic dye reaction to laser reaction. 5. After 1-4 experimental studies, the study wsa explore the effects of laser irradiation on the organisms, alone and in combination with prior treatment with dyes. The growth of MRSA was inhibited by argon laser wiht or without dyes (MB,PSP,ICG). And zone of growth inhibition treated with dye became distinct. Further attempts were done to know the mechanism of bacterial destruction by argon laser irradiation. And following results were comfirmed : 1. Not only MRSA,but MSSA,E.Cloli and P.S.were destructed by the argon laserirradiation. Moreover, P.S.was destructed with a half energy of laser irradiation. 2. The destruction of bacteria with laser depended on the total Joules, not on peak power. 3. From TEM observation, cytoplasma an DNA oflased MRSA was changed the structure and density. But bacteriolysys was not occurred. These changes were a little similar to the image of heat damaged MRSA compared with that of UV damaged MRSA.4. From stained method with acrigine-orange whitch is able to stain DNA and RNA,and electrophoresis, the structure of DNA was not destructed. From our results, we guess that initial trigger point of laser destruction exists in cytoplasma.

本研究的目的是建立针对 MRSA 感染引起的化脓性疾病的激光疗法。这项研究的目的是寻求一种光化学杀菌疗法，包括激光照射与染料相结合的热效应。首先进行了初步实验，了解染料和MRSA.1的基本信息。染料溶液的分光光度测定。 2. 细菌生长抑制作用和细菌染色。 3.细菌的热敏感性。 4.放热染料反应到激光反应。 5. 经过 1-4 项实验研究，该研究探讨了单独使用激光照射以及与预先使用染料处理相结合的激光照射对生物体的影响。使用或不使用染料（MB、PSP、ICG）的氩激光可抑制 MRSA 的生长。经染料处理后的生长抑制区变得清晰。进一步尝试了解氩激光照射破坏细菌的机制。并证实了以下结果： 1.氩激光照射不仅能破坏MRSA，还能破坏MSSA、E.Cloli和P.S.。而且，P.S.用一半能量的激光照射就被破坏。 2. 激光对细菌的杀灭取决于总焦耳数，而不是峰值功率。 3.从TEM观察，MRSA脱氧后的细胞质和DNA发生了结构和密度的改变。但没有发生溶菌。与紫外线损伤的 MRSA.4 相比，这些变化与热损伤的 MRSA 的图像有点相似。从可染色DNA和RNA的吖啶橙染色方法和电泳来看，DNA的结构没有被破坏。根据我们的结果，我们猜测激光破坏的初始触发点存在于细胞质中。