"The Cell Destruction with Super Pulse Laser Under the Different Bands of Wave Length. "

本研究的目的是阐明超脉冲激光破坏细胞的机理，为超脉冲激光在医学上的应用奠定基础。经低能量超脉冲激光照射后的肌细胞经电子显微镜观察为：(1)红外区无变化，(2)绿区线粒体扩张，(3)紫外区线粒体空泡化。超强脉冲激光的组织反应：高能量的超脉冲激光照射后，肝、脾的组织反应被认为是由(1)红外波段的热产生和(2)绿光和紫外线波段的直接光子效应或冲击波效应引起的。超脉冲激光在医学上的应用。(1)脊柱内窥镜激光手术，在脊柱内窥镜视野下可用超脉冲激光切断桡神经。(2)关节镜激光手术，用超脉冲激光观察关节组织反应，进行切割、凝固和汽化。(3)内窥镜激光心脏手术，研究激光对人体二尖瓣的反应，以选择激光和照射条件。

The objectives of this study are to clarify the mechanism of cell destruction with super pulse laser, and perform the basic study for the medical application of super pulse laser.The following results were obtained.1. Cell destruction by super pulse laser.The change of the muscle cell after the irradiation of super pulse laser with low energy were (1) nothing in infra-red band, (2) mitochondrial expansion in green band and (3) mitochondrial vacuolation in ultra-violet band by the electro-micrographical observation.2. Tissue reaction by super pulse laser.The tissue reaction in liver and spleen after the irradiation of super pulse laser with high energy were thought to be caused by (1) heat generation in infra-red band and (2) direct photon effect or shock wave effect in green and ultra-violet bands.3. Medical application of super pulse laser.(1) Spinal endoscopic laser surgery ; The radial nerves could be cut by super pulse laser under the spinal endoscopic visual field.(2) Arthroscopic laser surgery ; The joint tissue reaction was observed to be effective for cutting, coagulation and vaporization with super pulse laser.(3) Endoscopic Laser heart surgery ; The reaction of human mitral valve with laser were investigated to select the laser and irradiating condition.