"The Cell Destruction with Super Pulse Laser Under the Different Bands of Wave Length. "

“不同波长波段下超脉冲激光对细胞的破坏。”

• 批准号: 63440045
• 负责人: ABE Yusuke
• 金额: $ 18.5万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63440045/
• 关键词: Super pulse laser; Cell destruction; Tissue reaction; Endscope; Laser Surgery; Spinal Surgery; Arthroscopy; Heart Surgery; 脊髄手術; エキシマレ-ザ-; パルスYAGレ-ザ-; 生体作用; 選択的破壊; ミトコンドリア; スーパーパルスレーザー; パルスYAGレーザー; エキシマレーザ

The objectives of this study are to clarify the mechanism of cell destruction with super pulse laser, and perform the basic study for the medical application of super pulse laser.The following results were obtained.1. Cell destruction by super pulse laser.The change of the muscle cell after the irradiation of super pulse laser with low energy were (1) nothing in infra-red band, (2) mitochondrial expansion in green band and (3) mitochondrial vacuolation in ultra-violet band by the electro-micrographical observation.2. Tissue reaction by super pulse laser.The tissue reaction in liver and spleen after the irradiation of super pulse laser with high energy were thought to be caused by (1) heat generation in infra-red band and (2) direct photon effect or shock wave effect in green and ultra-violet bands.3. Medical application of super pulse laser.(1) Spinal endoscopic laser surgery ; The radial nerves could be cut by super pulse laser under the spinal endoscopic visual field.(2) Arthroscopic laser surgery ; The joint tissue reaction was observed to be effective for cutting, coagulation and vaporization with super pulse laser.(3) Endoscopic Laser heart surgery ; The reaction of human mitral valve with laser were investigated to select the laser and irradiating condition.

本研究旨在阐明超脉冲激光对细胞的破坏机理，为超脉冲激光在医学上的应用做基础研究，取得了以下研究结果.超脉冲激光对细胞的破坏：电镜观察低能量超脉冲激光照射后肌细胞的变化：（1）红外波段无变化，（2）绿色波段线粒体扩张，（3）紫外波段线粒体空泡化.超脉冲激光引起的组织反应：高能超脉冲激光照射肝脏和脾脏引起的组织反应主要是由于（1）红外波段的发热和（2）绿色和紫外波段的直接光子效应或冲击波效应.超脉冲激光的医学应用。(1)脊柱内窥镜激光手术：在脊柱内窥镜视野下，用超脉冲激光切断桡神经。(2)关节镜下激光手术;观察到关节组织反应对超脉冲激光的切割、凝固和汽化有效。(3)激光心脏手术：研究激光对人二尖瓣的作用，选择激光和照射条件。

项目成果

河野明正: "ス-パ-パルスレ-ザによる生体組織の破壊作用" 医用電子と生体工学. 28(特別号). (1990)

Akimasa Kono：“超脉冲激光对生物组织的破坏”《医疗电子和生物工程》28（特刊）（1990 年）。

河野 明正: "ス-パ-パルス-レ-ザ-の組織切離における熱作用に関する研究" 日本レ-ザ-医学会誌. 11(3). (1991)

Akimasa Kono：“组织切割中超脉冲激光的热效应研究”日本激光医学会杂志 11（3）（1991）。

Y. Abe: "Laser angioplasty with excimer laser" J. of Japan Soc. for Laser Med.10(1). 31-36 (1989)

Y. Abe：“准分子激光激光血管成形术”J. of Japan Soc。

Y. Abe, et al: "Reaction of super pulse laser in cell destruction under different band of wave length" J. of Japan Soc. for Laser Med.10(3). 501-504 (1989)

Y. Abe等人：“超脉冲激光在不同波长带下细胞破坏中的反应”J. of Japan Soc。

米沢卓実: "ス-パ-パルスレ-ザ-の整形外科応用に関する基礎的研究" 医用電子と生体工学. 28(特別号). (1990)

Takumi Yonezawa：“超脉冲激光在骨科手术中的应用的基础研究”医学电子和生物工程28（特刊）（1990）。