Immunoproliferative Microenvironment in the Spleen-An Immunohistological Study.

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(Aim of this study) We examined immunohistologically the cellular rosettes formed by ED2-positive macrophages and antibody forming cells in the rat spleen. The aim of this study is to know the correlation between various types of immune response and the rosette formation and to consider the functional role of the rosette as an immunoproliferative microenvironment.(Results)(1992)(1) We demonstrated that ED2-positive macrophages also form rosettes with erythroblasts in the red pulp cord (Published just before getting this grant). (2) We examined isotypes of anti-FITC antibody forming cells and found that they bore mainly an IgM isotype (unpublished data). (3) The immunoelectron microscopical analysis is now undergoing.(1993)(1) We prepared an antigen, FITC-hemocyanin, and found that antibody forming cells to this antigen also formed rosettes with ED2-positive macrophages in the outer PALS (unpublished data). We also studied Plasmodium-infected rat spleens. We found that antibody forming … More cells and ED2-positive macrophages considerably increased in number and formed rosettes together in the PALS (presented at an international congress, in preparation). (2) This project stands on a still preliminary field of research, "Immunohistology", which needs further study to be established. However, we propose this kind of approach as a new and very important one. In order to demonstrate some crops of this field, we have produced and studied a rat experimental pancreatitis model. We found a massive increase of macrophages in the inflamed tissues (Gastroenterology, 1993) and demonstrated a proliferation of macrophage subpopulations by the double immunostaining technique (Arch.Histol.Cytol., 1993).(DISCUSSION AND FUTURE PROJECT) We consider that the rosette formation of ED2-positive macrophages and antibody forming cells is a common phenomenon producing the microenvironment for the antibody forming cell response not only to various types of artificial antigen but also to infectious microorganisms. Furthermore, they may slso constitute the microenvironment for hemopoietic cell lineages. We plan to immunostain the rosettes with double color fluorescent probes and examine the three dimensional structure of them with the aid of a confocal laser scanning microscope. Less