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Mechanism for a dendritic cell transport in the hepatic sinusoids and lymph.

树突状细胞在肝窦和淋巴中的运输机制。

基本信息

批准号：
09670019
负责人：
MATSUNO Kenjiro
金额：
$ 2.05万
依托单位：
KUMAMOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670019/
关键词：
Liver Hepatic Sinusoids Hepatic Lymph Dendritic Cells Migratory Pathway Kupffer Cells Cell Adhesion Molecules

项目摘要

Aim of this study : Liver is constantly surveyed by a traffic of antigen-presenting dendritic cells (DC). We studied about the transport system of DC within the liver microarehitecture and factors which regulate this transport.Result 1. intravenously transferrd rat DC translocated and selectively accumulated in the paracortex of the hepatic lymph nodes (published). Soon after the rat liver transplantation, donor MHCII+ cells migrated to both T and B cell areas of thc spleen. The hepatic lymph leaked into the peritoncal cavity and DC in lymph were absorbed into diaphragmatic lymph plexus and transported to the anterior mediastinal LN.Donor MHCII+ cells arc speculated to correspond to DC progenitors in the liver (in preparation).Result 2. A large number of rat DC were injected intravenously to allogencic host and a fate of the DC in the liver tissue was traced lime kinetically. We found that DC were first entrapped by the Kupffer cells in the sinusoidal wall possibly through mechanical and molecular interactions. The entrapped DC may extravasate into the Disse's space and then exit the hepatic lobule into tissue space of the portal area. Eventually these cells probably crawl into the lymphatic capillary located in the portal area (presented at 103rd Japanese Anatomy Congress). This will be confinned by in vivo observation using confocal laser microlluorography by a collaboration with Dr. M.Suematsu, Keio University (now under study).Result 3. In the sinusoidal area a close association between the DC and Kupffer cells is often observed in normal rats. Rat Kupffer cells in the hepatie sinusoids are capable of selectively trapping DC from the blood in vivo and in vitro (published) suggesting a presence of certain adhesion molecules, possibly sugar residues and leetins. By this, DC could attach to the sinusoidal wail probably as a prelude to extravasation. This is now under study by collaborating with Dr. T.Suganuma. Miyazaki Medical College.

这项研究的目的：通过抗原提呈树突状细胞(DC)的交通不断地观察肝脏。我们对DC在肝脏微结构中的转运系统及其调控因素进行了研究。结果1.静脉移植大鼠DC在肝淋巴结的副皮质内发生移位并选择性聚集。大鼠肝移植后不久，供者的MHCII+细胞向脾的T和B细胞区迁移。漏入腹膜腔的肝淋巴和淋巴中的DC被吸收到横隔淋巴丛并转运到前纵隔淋巴结。供体MHCII+细胞可能对应于肝脏中的DC前体细胞(正在制备中)。结果2.将大量的大鼠DC静脉注射到同种异体宿主体内，并动态追踪DC在肝组织中的去向。我们发现DC首先被库普弗细胞包裹在窦壁中，可能是通过机械和分子相互作用。包裹的DC可渗入Disse‘s间隙，然后离开肝小叶进入汇管区的组织间隙。最终，这些细胞可能会爬进位于汇管区的毛细淋巴管(在第103届日本解剖学大会上发表)。这将受限于与庆应大学(现正在研究中)的M.Suematsu博士合作使用激光共聚焦显微造影术进行的活体观察。结果3.在正常大鼠的肝窦区，DC和Kupffer细胞之间经常观察到密切的联系。肝窦中的大鼠Kupffer细胞能够选择性地捕获体内和体外血液中的DC(已发表)，表明存在某些黏附分子，可能是糖残留物和Leetins。因此，DC可能附着在正弦哀号上，可能是渗出的前奏。这项研究目前正在与T.Suganuma博士合作进行。宫崎骏医科大学。