Stem cells of the dendritic cell lineage in the liver : demonstration and analysis

肝脏树突状细胞谱系的干细胞：演示和分析

• 批准号: 15390059
• 负责人: MATSUNO Kenjiro
• 金额: $ 9.22万
• 依托单位: Dokkyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390059/
• 关键词: liver; dendritic cell; stem cell; liver transplantation; congeneic animal; transmigration; allosensitization; rat; stem cell; 肝移殖

1.Soon after the liver transplantation, graft-derived DCs with a very immature phenotype, TcR- Ig- MHCI+ MHCII+ ICAM1+ CD11b- CD11c- αEβ7 integrin- and CD86-, performed a diffuse transmigration through the HEV and splenic marginal sinus of host lymphoid tissues. These DCs clustered with host T cells where a massive host proliferative response started.2.In addition, MHCII+ Ig- B220+ cells with plasmacytoid DC phenotype and MHCII+ TcR+ cells as well as MHCII- T cells and Ig+ B cells concomitantly transmigrated in a similar fashion as the mice model (ref 3, 7).3&6.While the bone marrow DC fraction transmigrated to host lymphoid tissues after i.v. transfer, the liver crude DC fraction did not, probably because the isolation procedure including enzymal digestion caused cell injury.4.The tolerance-inducing regimen, donor-specific transfusion (DST) 7 d before operation completely blocked this transmigration and considerably suppressed the proliferative response of host T cells there. The DST regimen itself induced antibody forming-cell response in the host spleen, leading to donor-specific IgM and IgG_1 cytotoxic alloantibody production. This caused a rapid elimination of migrating donor cells in the blood. Since intragraft T cell apoptosis is reported after the murine liver transplantation, we consider that the intrahepatic sensitization without effector T cell production in the host lymphoid tissues may induce the abortive T cell response. The deletion may exceed the production of alloreactive T cells, resulting in the liver operational tolerance.5.The donor cells persisted up to 2 years after liver transplantation from the congeneic donor rats. Few of them bore DC phenotype.

1.肝移植后不久，TcR-Ig-MHCI+MHCII+ICAM1+CD11b-CD11c-αEβ7整合素和CD86-表型的移植物来源DC通过宿主淋巴组织的戊型肝炎病毒和脾边缘窦进行了弥漫性迁移。此外，具有浆细胞样树突状细胞表型的MHCII+Ig-B220+细胞和MHCII+TCR+细胞以及MHCII-T细胞和Ig+B细胞的迁移方式与小鼠模型相似(参考文献3，7)。3&6.静脉注射后，骨髓DC部分向宿主淋巴组织迁移。4.耐受诱导方案，供体特异性输血(DST)在术前7d完全阻断了这种移植，并显著抑制了宿主T细胞的增殖反应。DST方案本身在宿主脾中诱导抗体形成细胞反应，导致供体特异性的IgM和Ig1细胞毒同种抗体的产生。这导致血液中迁移的供体细胞迅速消除。由于小鼠肝移植后移植物内T细胞凋亡的报道，我们认为肝内致敏而宿主淋巴组织中没有产生效应性T细胞可能导致了流产的T细胞反应。这种缺失可能会超过同种异体反应性T细胞的产量，导致肝脏手术耐受。5.同种供体大鼠肝移植后供体细胞存活至2年。少数细胞具有DC表型。

项目成果

多様で多彩なマクロファージ系細胞

多样化和多功能的巨噬细胞

• 发表时间: 2004
• 期刊: Molecular Medicine 41・8
• 作者: Shimaoka;T.;その間10名;Yonehara;S.;Yoneyama H;Tahara K;Ezaki T;松野健二郎;松野健二郎
• 通讯作者: 松野健二郎

松野健二郎: "樹状細胞の体内動態"現代医療. 35(8). 1783-1788 (2003)

Kenjiro Matsuno：“树突状细胞的身体动力学”现代医学35（8）1783-1788（2003）。

Plasmacytoid DCs help lymph node DCs to induce anti-HSV CTLs.

浆细胞样 DC 帮助淋巴结 DC 诱导抗 HSV CTL。

• DOI: 10.1084/jem.20041961
• 发表时间: 2005-08-01
• 期刊: JOURNAL OF EXPERIMENTAL MEDICINE
• 影响因子: 15.3
• 作者: Yoneyama, H;Matsuno, K;Toda, E;Nishiwaki, T;Matsuo, N;Nakano, A;Narumi, S;Lu, B;Gerard, C;Ishikawa, S;Matsushima, K
• 通讯作者: Matsushima, K

Characterization of adjuvant-induced rat lymphangiomas as a Model to study the lymph drainage from abdominal cavity.

佐剂诱导的大鼠淋巴管瘤的表征作为研究腹腔淋巴引流的模型。

• 发表时间: 2004
• 期刊: Jap.J.Lymphol. 27・1
• 作者: Shimaoka;T.;その間10名;Yonehara;S.;Yoneyama H;Tahara K;Ezaki T
• 通讯作者: Ezaki T

樹状細胞(DC)とケモカイン

树突状细胞 (DC) 和趋化因子

• 发表时间: 2005
• 期刊: 細胞 37・13
• 作者: Matsuno K;Walter BH;上田 裕司;Matsuno K.;Walter BA.;Matsumo K;Yoneyama H;Tahara K;Yoneyama H;上田 裕司
• 通讯作者: 上田 裕司