Dendritic Cell Dynamics in the Liver and Hepatic Lymph

肝脏和肝淋巴中的树突状细胞动力学

• 批准号: 12470004
• 负责人: MATSUNO Kenjiro
• 金额: $ 4.74万
• 依托单位: Dokkyo University School of Medicine (2001)Kumamoto University (2000)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470004/
• 关键词: Liver; Hepatic Lymph; Dendritic Cell; Dendritic Cell Precursor; Cell Trafficking; Hepatic Sinusoids; Kupffer Cell; C-type Lectin

(1) Turnover of interstitial dendritic cells (DC) in the rat liver is in the order of 2-3 wk. DC in the liver (interstitial DC) capture and process antigens, enter the draining lymph (DC in hepatic lymph) and accumulate in the T cell area of hepatic lymph nodes (REFERENCE 1).(2) DC precursors could be recruited to the liver after intravenous injection of latex (rats, REF.2) or P. acnes bacilli (mice, REF.4,8).(3) Intravenously injected DC soon appeared in the liver and displayed a sudden arrest in sinusoids in periportal regions. These DC mostly bound to Kupffer cells and then moved to the portal area by 4 hr (REF. 2,9).(4) Both DC precursors and DCs at the antigen-transporting stage could be recruited to the liver and their majority initially showed a selective binding to Kupffer cells through N-acetylgalactosamine-specific C-type lectin-like receptors (REF.2, 3).(5) Donor MHCII+ cells in host lymphoid tissues after liver allotransplantation have a phenotype of immature DC and may correspond to rat DC progenitors in the liver (in preparation).(7) When allogeneic DCs were intravenously injected, or heart graft was transplanted, donor DCs formed clusters with proliferating host T lymphoblasts (rosettes) in hepatic lymph nodes where the T-cell proliferative response started (REF.5, 6).In summary, DC system in the liver is highly elaborated and tightly connected to the immune system, contributing to not only local defense but also systemic host defense to blood-borne antigens.

(1)大鼠肝脏间质树突状细胞（DC）的更新约为2-3周。肝脏中的DC（间质DC）捕获并加工抗原，进入引流淋巴（肝淋巴中的DC）并积聚在肝淋巴结的T细胞区（参考文献1）。(2)静脉注射乳胶（大鼠，参考文献2）或痤疮丙酸杆菌（小鼠，参考文献4、8）后，DC前体可募集至肝脏。(3)腹腔注射DC后，DC很快出现在肝脏中，并在门静脉周围区域的窦状隙中显示突然停止。这些DC主要与枯否细胞结合，然后在4小时内移动到汇管区（参考文献2，9）。(4)DC前体和抗原转运阶段的DC都可以被招募到肝脏，并且它们中的大多数最初通过N-乙酰半乳糖胺特异性C型凝集素样受体与枯否细胞选择性结合（参考文献2、3）。(5)同种异体肝移植后宿主淋巴组织中的供体MHCII+细胞具有未成熟DC的表型，可能对应于肝脏中的大鼠DC祖细胞（制备中）。(7)当静脉注射同种异体DC或移植心脏时，供体DC在肝淋巴结中与增殖的宿主T淋巴母细胞（roblasts）形成簇，其中T细胞增殖反应开始（参考文献5，6）。总之，肝脏中的DC系统高度精细且与免疫系统紧密连接，不仅有助于局部防御，而且有助于全身宿主防御血源性抗原。

项目成果

Saiki, T.: "In Vivo Roles of Donor and Host Dendritic Cells in Allogeneic Immune Response : Cluster Formation with Host Proliferating T-Cells"J.Leukoc.Biol.. 69. 705-712 (2001)

Saiki, T.：“供体和宿主树突细胞在同种异体免疫反应中的体内作用：宿主增殖 T 细胞的簇形成”J.Leukoc.Biol.. 69. 705-712 (2001)

Uwatoku, R.: "Asialoglycoprotein Receptors on Rat Dendritic Cells: Possible Roles for Binding with Kupffer Cells and Ingesting Virus Particles"Arch.Histol.Cytol.. 64. 223-232 (2001)

Uwatoku, R.：“大鼠树突状细胞上的亚洲糖蛋白受体：与库普弗细胞结合和摄入病毒颗粒的可能作用”Arch.Histol.Cytol.. 64. 223-232 (2001)

Nomiyama, H.: "Human CC chemokine liver-expressed chmokine/CC16 is a functional ligand for CCR1, CCR2 and CCR5 and constitutively expressed by hepatocytes"Int.Immunol.. 13・8. 1021-1029 (2001)

Nomiyama, H.：“人CC趋化因子肝脏表达的趋化因子/CC16是CCR1、CCR2和CCR5的功能性配体，并且由肝细胞组成型表达”Int.Immunol.. 13・8 (2001)。

Matsuno K.: "Trafficking of Dendritic cells"Igaku no Ayumi. 200. 472-476 (2002)

松野 K.：“树突状细胞的贩运”Igaku no Ayumi。

Saiki, T.: "Trafficking of Host-and Donor-Derived Dendritic Cells in the Rat Cardiac Transplantation : Allosensitization in the Spleen and Hepatic Nodes"Transplantation. 71. 1806-1815 (2001)

Saiki, T.：“大鼠心脏移植中宿主和供体衍生的树突状细胞的贩运：脾脏和肝结节的同种异体致敏”移植。