Establishment of the Analytical Techniques for Various Chemicals in Human Tissues

一种可靠和敏感的方法已经开发评估催眠药物三唑仑的浓度在人体组织，包括腐烂的组织。使用腐烂的组织对三唑仑的毒理学效应进行了法医研究。为了找到最合适的组织样本进行毒理学分析，我们用大鼠检测了死后三唑仑在人体组织中的浓度变化。脾、腹肌、肝、肾三唑仑浓度显著升高，血、肺三唑仑浓度略有升高。另一方面，在大腿肌肉或大脑中没有观察到任何变化，大腿肌肉的值与死后立即收集的血液样本中的值相似。结果表明，死后三唑仑通过胃壁扩散到周围组织中，因此，除了血液外，还必须分析大腿肌肉和大脑，才能正确诊断是否摄入了三唑仑。建立了一种简便、灵敏、可靠、经济的同时测定人体生物材料中二氯百草枯和二溴双喹菊酯的高效液相色谱法。我们用这种方法来检查百草枯和diquat在尸体解剖个体组织中的浓度。

A reliable and sensitive method has been developed to assess the concentrations of the hypnotic drug triazolam in human tissues, including putrefied tissues. A forensic study was performed on the toxicological effects of triazolam using putrefied tissues.Postmortem changes of triazolam concentrations in body tissues were examined using rats, in order to find the most suitable tissue samples for toxicological analysis. Triazolam concentrations were markedly increased in the spleen, the abdominal muscle, the liver and the kindey, and were slightly increased in the blood and the lung. On the other hand, no changes were observed in the thigh muscle or the brain, and the value in the thigh muscle was similar to those in the blood samples collected immediately after death. The results indicate that triazolam diffuses into the surrounding tissues through the stomach wall after death, so that the thigh muscle and the brain have to be analyzed as well as the blood for a correct diagnosis of triazolam ingestion.A simple, sensitive, reliable, and economical method for simultaneous determination of paraquat dichloride and diquat dibromide in human biological materials was developed, using high performance liquid chromatography. We used this method to examine the concentrations of paraquat and diquat in tissues of an autopsied individual.