Establishment of the Analytical Techniques for Various Chemicals in Human Tissues

人体组织中多种化学物质分析技术的建立

• 批准号: 04807042
• 负责人: KUDO Keiko
• 金额: $ 1.28万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04807042/
• 关键词: poisonong; triazolam; postmortem change; GC; paraquat; diquat; HPLC

A reliable and sensitive method has been developed to assess the concentrations of the hypnotic drug triazolam in human tissues, including putrefied tissues. A forensic study was performed on the toxicological effects of triazolam using putrefied tissues.Postmortem changes of triazolam concentrations in body tissues were examined using rats, in order to find the most suitable tissue samples for toxicological analysis. Triazolam concentrations were markedly increased in the spleen, the abdominal muscle, the liver and the kindey, and were slightly increased in the blood and the lung. On the other hand, no changes were observed in the thigh muscle or the brain, and the value in the thigh muscle was similar to those in the blood samples collected immediately after death. The results indicate that triazolam diffuses into the surrounding tissues through the stomach wall after death, so that the thigh muscle and the brain have to be analyzed as well as the blood for a correct diagnosis of triazolam ingestion.A simple, sensitive, reliable, and economical method for simultaneous determination of paraquat dichloride and diquat dibromide in human biological materials was developed, using high performance liquid chromatography. We used this method to examine the concentrations of paraquat and diquat in tissues of an autopsied individual.

建立了一种可靠和灵敏的方法来评估催眠药三唑仑在人体组织中的浓度，包括腐烂的组织。利用腐烂的组织对三唑仑的毒理作用进行了法医学研究，并用大鼠检测了三唑仑在体内组织中的浓度变化，以寻找最适合进行毒理学分析的组织样品。三唑仑在脾、腹肌、肝脏和肾脏中的浓度显著升高，在血液和肺中的浓度略有升高。另一方面，大腿肌肉或大脑没有观察到变化，大腿肌肉的数值与死亡后立即采集的血液样本中的数值相似。结果表明，三唑仑死后通过胃壁扩散到周围组织，因此必须对大腿肌肉和脑以及血液进行分析，才能正确诊断三唑仑摄入。建立了一种简便、灵敏、可靠、经济的同时测定人体生物材料中百草枯和敌草枯的方法。我们使用这种方法检测了一名尸检人员组织中百草枯和敌草枯的浓度。

项目成果

Ito, S., and Imamura, T.: "Simultaneous determination of paraquat and diquat in human tissues by high-performance liquid chromatography." J.Chromatogr.Biomed.Appl.617. 119-123 (1993)

Ito, S. 和 Imamura, T.：“通过高效液相色谱法同时测定人体组织中的百草枯和敌草快。”

Kudo,K.: "Postmortem changes of triazolam concentiations in body tissues." Japanese Journal of Legal Medicine. 46. 293-296 (1992)

Kudo,K.：“死后身体组织中三唑仑浓度的变化。”

Ito,S.: "Simultaneous determination of paraquat and diquat in human tissues by high-performance liquid chromatography" Journal of Chromatography Bimedical Applications. 617. 119-123 (1993)

Ito,S.：“通过高效液相色谱同时测定人体组织中的百草枯和敌草快”，色谱双医学应用杂志。

Shinji Ito: "Simultaneous determination of paraquat and diquat in human tissues by high-performance liquid chromatography" Journal of Chromotography,Biomedical Applications. 617. 119-123 (1993)

Shinji Ito：“用高效液相色谱法同时测定人体组织中的百草枯和敌草快”，色谱法杂志，生物医学应用。

Kudo, K,Imamura, T.and Urakawa, N: "Postmortem changes of triazolam concentrations in body tissues." Jpn.J.Legal Med.46. 293-296 (1992)

Kudo, K、Imamura, T. 和 Urakawa, N：“死后身体组织中三唑仑浓度的变化。”