Establishment of a rapid screening procedure of abused drugs and its forensic toxicological application

滥用药物快速筛查程序的建立及其法医毒理学应用

• 批准号: 16390194
• 负责人: KUDO Keiko
• 金额: $ 8.45万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390194/
• 关键词: drug abuse; drug screening; GC; MS; abused drug; MDMA; morphine; methamphetamine; 規制薬物; 脱法ドラッグ; ガスクロマトグラフィー; 質量分析

We devised a rapid screening method for and simultaneous semi quantitative analysis of 30 abused drugs including amphetamines, amphetamine-, piperazine-, tryptamine-, and phenethylamine-derived designer drugs and opiates in human urine. The urine sample was digested with urease, and the drugs were analyzed by gas chromatography-mass spectrometry in the scan mode after solid phase extraction with Focus^<TM> column and acetylation. Retention time obtained with use of retention time locking technique and 3 qualifier ions were used to obtain positive results. Based on the established method, database of "abused drugs" was constructed from parameters in each drug including retention time, QT%, slope and intercept of calibration curve and mass spectrum using tentative quantification software NAGINATA^<TM>. We examined the usefulness of the database in forensic toxicological cases and found that this new technique was simple and gave useful information on the existence as well as amount of drugs in the sample without having standard compounds.We also established the reliable quantification procedures for 13 amphetamine-related drugs in whole blood,2 tryptamines in human whole blood and urine and morphine in human liver and kidney. The methods were used for diagnosing cause of death in forensic autopsy cases.

我们设计了一种快速筛查方法，并同时半定量分析30滥用药物，包括安非他明，安非他明，哌嗪，色胺，苯乙胺衍生的设计师药物和阿片类药物在人尿中。尿样经尿素酶消化后，经Focus柱固相萃取、乙酰化后，用气相色谱-质谱联用仪扫描分析<TM>。采用保留时间锁定技术获得保留时间，并使用3种限定离子获得阳性结果。在此基础上，利用NAGINATA ®定量软件，对每种药物的保留时间、QT%、校正曲线的斜率和截距以及质谱等参数进行了初步的定量分析，建立了“滥用药物”数据库<TM>。该方法简便、快速，无需标准物质即可提供样品中毒品的存在和含量信息，并建立了全血中13种苯丙胺类药物、全血和尿液中2种色胺类药物、肝脏和肾脏中吗啡类药物的定量分析方法。并应用于法医学尸体解剖的死因诊断。

项目成果

Simple and sensitive determination of free and total morphine in human liver and kidney using gas chromatography-mass spectrometry

• DOI: 10.1016/j.jchromb.2005.10.049
• 发表时间: 2006-01-18
• 期刊: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
• 影响因子: 3
• 作者: Kudo, K;Ishida, T;Ikeda, N
• 通讯作者: Ikeda, N

Sensitive determination of alpha-methyltryptamine (AMT) and 5-methoxy-N, N-diisopropyltryptamine (5MeO-DIPT) in whole blood and urine using gas chromatography-mass spectrometry

使用气相色谱-质谱法灵敏测定全血和尿液中的 α-甲基色胺 (AMT) 和 5-甲氧基-N, N-二异丙基色胺 (5MeO-DIPT)

• 发表时间: 2005
• 期刊: Journal of Chromatography B 823
• 作者: Ohara M;et al.;Kudo et al.;Ishida T et al.
• 通讯作者: Ishida T et al.