Establishment of a rapid screening procedure of abused drugs and its forensic toxicological application

点击翻译按钮获取中文摘要

We devised a rapid screening method for and simultaneous semi quantitative analysis of 30 abused drugs including amphetamines, amphetamine-, piperazine-, tryptamine-, and phenethylamine-derived designer drugs and opiates in human urine. The urine sample was digested with urease, and the drugs were analyzed by gas chromatography-mass spectrometry in the scan mode after solid phase extraction with Focus^<TM> column and acetylation. Retention time obtained with use of retention time locking technique and 3 qualifier ions were used to obtain positive results. Based on the established method, database of "abused drugs" was constructed from parameters in each drug including retention time, QT%, slope and intercept of calibration curve and mass spectrum using tentative quantification software NAGINATA^<TM>. We examined the usefulness of the database in forensic toxicological cases and found that this new technique was simple and gave useful information on the existence as well as amount of drugs in the sample without having standard compounds.We also established the reliable quantification procedures for 13 amphetamine-related drugs in whole blood,2 tryptamines in human whole blood and urine and morphine in human liver and kidney. The methods were used for diagnosing cause of death in forensic autopsy cases.