Molecular structural studies of gap junction protein compositions.
间隙连接蛋白组合物的分子结构研究。
基本信息
- 批准号:05454136
- 负责人:
- 金额:$ 3.46万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1993
- 资助国家:日本
- 起止时间:1993 至 1994
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Gap junction proteins of connexin (Cx) family were examined by using specific rabbit antibodies raised against type-specific peptide sequence domains at the single connexon channel level and on the tissue distiribution and cell type secificity.1. Single gap junction channel (connexon) particles isolated from rat and guinea pig liver by mild detergent extraction showed doughnut-shaped unit particles with central depressions in negative staining and low angle rotary shadowing electron microscopy. Further purification by high performance liquid chromatography followed by affinity chromatogram on either anti Cx26 of anti-Cx32 specific antibody column yielded single western blot pattern without appreciable cosedimentations of other connexins. This result may indicate that single connexon hexamers are composed of either Cx26 of Cx32 only.2. In contrast to the salivary glands which contained both Cx32 and Cx26, lipid secreting Harderian glands showed only Cx26 immunofluorescence in the acinar cells. Myoepithelial cells showed Cx43. In mammary glands, Cx26 and Cx32 in acinar cells and Cx43 in myoepithelial cells revealed dynamic changes of expression during pregnancy, parturition, lactation and weaning period at RNA transcription, protein production, and phosphorylation states, indicating the activity-related expression of gap junction proteins thus consituting functionally distinct cell groups.These results indicate that differential expressions of various gap junction proteins are closely correlated with cell type specific functions and activities.
利用特异性兔抗体,在单个连接子通道水平、组织分布和细胞类型特异性上,检测了连接蛋白(Cx)家族的缝隙连接蛋白.通过温和去污剂提取从大鼠和豚鼠肝脏分离的单个间隙连接通道(connexon)颗粒在负染色和低角度旋转阴影电子显微镜下显示具有中央凹陷的甜甜圈状单位颗粒。通过高效液相色谱法进一步纯化,然后在抗Cx 26或抗Cx 32特异性抗体柱上进行亲和色谱,得到单一的蛋白质印迹模式,没有明显的其他连接蛋白的共沉淀。这一结果可能表明单个连接子六聚体仅由Cx 26或Cx 32组成.与含有Cx 32和Cx 26的唾液腺相反,分泌脂质的哈氏腺腺泡细胞中仅显示Cx 26免疫荧光。肌上皮细胞显示Cx43。在乳腺中,腺泡细胞中的Cx 26和Cx 32以及肌上皮细胞中的Cx43在RNA转录、蛋白质产生和磷酸化状态方面显示出在妊娠期、分娩期、哺乳期和断奶期的动态变化,显示活动-这些结果表明,不同的间隙连接蛋白的差异表达,与细胞类型特异性功能和活性密切相关。
项目成果
期刊论文数量(48)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
柴田洋三郎・倉岡晃夫: "細胞間連絡チャネル・ギャップ結合-その構成分子コネキシンと組織特異性" 医学のあゆみ. 167. 161- (1993)
Yozaburo Shibata 和 Akio Kuraoka:“细胞间通讯通道和间隙连接 - 它们的组成分子连接蛋白和组织特异性”医学史 167. 161- (1993)。
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- 影响因子:0
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- 通讯作者:
Kuraoka,A: "characterization of isolatedguinea pig liver gap junction." Prog.Cell.Res.4. 111-114 (1994)
Kuraoka,A:“离体豚鼠肝脏间隙连接的表征。”
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- 影响因子:0
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T.Hatae,etal,: "Cytoplasmic surface ultrastruactures of gap jumctiurs in bouive leus fibers" Iuucstigative Ophthalmol.Visual Science. 34. 2164-2173 (1993)
T.Hatae 等人:“布乌伊路斯纤维间隙连接的细胞质表面超结构”Iuucstigative Olookingmol.Visual Science。
- DOI:
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- 影响因子:0
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Kuraoka,A: "Localizations of gap junction prateins,connexins 32 and 26, in the rat and guinea pig livers as revealed by quick-freeze,deep-etch immunoelectron microscopy." J.Histochem.Cytochem.41. 971-980 (1993)
Kuraoka,A:“通过快速冷冻、深蚀刻免疫电子显微镜揭示了大鼠和豚鼠肝脏中缝隙连接蛋白、连接蛋白 32 和 26 的定位。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
A.Kuraoka: "Localization of gap junction proteins, Connexins 32 and 26, in rat and guinea pig liver as revealed by quick-freeze, deep-etch immunoelectron microscopy." J.Histochem.Cytochem.41. 971-980 (1993)
A.Kuraoka:“通过快速冷冻、深蚀刻免疫电子显微镜揭示了大鼠和豚鼠肝脏中间隙连接蛋白 Connexin 32 和 26 的定位。”
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- 影响因子:0
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{{ truncateString('SHIBATA Yosaburo', 18)}}的其他基金
Comparative and molecular anatomical research of GAP junction-related molecules.
GAP连接相关分子的比较和分子解剖学研究。
- 批准号:
19390052 - 财政年份:2007
- 资助金额:
$ 3.46万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular anatomical research for the role of gap junction proteins in cardiac function.
间隙连接蛋白在心脏功能中作用的分子解剖学研究。
- 批准号:
17390052 - 财政年份:2005
- 资助金额:
$ 3.46万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular Anatomy of Functional Differentination Affected by Combination of Gap Junctions, Connexins
间隙连接、连接蛋白组合影响功能分化的分子解剖学
- 批准号:
15390057 - 财政年份:2003
- 资助金额:
$ 3.46万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular Anatomy of Gap Junction Expression Regulation Effect in Conditional Cx Knockout Mice
条件性 Cx 基因敲除小鼠间隙连接表达调控效应的分子解剖学
- 批准号:
13470004 - 财政年份:2001
- 资助金额:
$ 3.46万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
ギャップ結合蛋白遺伝子変異マウスの分子解剖学的研究
间隙连接蛋白基因突变小鼠的分子解剖学研究
- 批准号:
11470005 - 财政年份:1999
- 资助金额:
$ 3.46万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Molecular anatomy of functional structural expression of gap junctions.
间隙连接功能结构表达的分子解剖学。
- 批准号:
07407001 - 财政年份:1995
- 资助金额:
$ 3.46万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Development and Application of Atomic Force Microscopy to biological sciences.
原子力显微镜在生物科学中的发展和应用。
- 批准号:
07557002 - 财政年份:1995
- 资助金额:
$ 3.46万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Structural Studies of Tissue Specificity and Diversity of Gap Junctions.
组织特异性和间隙连接多样性的结构研究。
- 批准号:
03454117 - 财政年份:1991
- 资助金额:
$ 3.46万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Ultrastrructural Variations of Gap Junctions Correlated with Tissue Types or Funtions.
间隙连接的超微结构变化与组织类型或功能相关。
- 批准号:
61570012 - 财政年份:1986
- 资助金额:
$ 3.46万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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