Ultrastrructural Variations of Gap Junctions Correlated with Tissue Types or Funtions.

间隙连接的超微结构变化与组织类型或功能相关。

• 批准号: 61570012
• 负责人: SHIBATA Yosaburo
• 金额: $ 1.34万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570012/
• 关键词: Gap junction; Nexus; intercellular communication; cell coupling; cardiac muscle cells; hepatocyte; myometrium; 組織特異性; 子宮平滑筋

1. A simple new method of deep-etching, upside-down etching was developed in an attempt to obtain clearer images of replicas, especially of fresh untreated tissues. During deep-etching, specimens were kept downwards and shaken periodically so that non-volatile materials in the subliming ice could fall out of the frozen tissues. This improvemnet leads to a considerable reduction of sedimented debris of unetchable materials on the membrane surfaces of gap junction plaques.2. Our previous rapid-freeze, deep-etch replica studies have revealed the differences of cytoplasmic surfacesbetween heart and liver gap junction. Further studies were carried out to clarify these differences in various types of tissues.In surface mucous cells of stomach, absorptive epithelial cells of small and large intestine, and distal tubule cells of kidney, similar to the liver gap junctions, most of the cytoplasmic surface membranes were monparticulate. Whereas, in the cardiac atrium and ventricle, aortic endothe … More lium, and the ciliary process, cytoplasmic surface membrane of these non-epithelial gap junctions consistently revealed characteristic particulate patterns like a tiled surface made up of round tiles of vaious sizes. Close examinations disclosed that those granular materials were not merely attached to the membrane surface but protruded from the membrane interior as an integral component of gap junction particles. In the same replica area of pregnant rat uterus at labor, we found that surface membrane of myometrial smooth muscle gap junctions were particulate, but those of endometrial epithelium were smooth.3. The results of present study strongly suggest that cytoplasmic surface structures are different between epithelial and non-epithelial gap junctions; the non-epithelial gap junctions contain the extra cytoplasmic surface domain that is absent in the gap junctions of epithelial origin.It is interesting to note the similar distribution tendency of intermediate filament proteins between epithelial and non-epithelial tissues. Less

1.一种简单的新方法，深蚀刻，倒置蚀刻的发展，试图获得更清晰的图像的副本，特别是新鲜的未经处理的组织。在深蚀刻过程中，标本保持向下并定期摇动，以便升华冰中的非挥发性物质可以从冷冻组织中脱落。这种改进导致间隙连接斑膜表面上不可蚀刻材料的沉积碎片显著减少。我们之前的快速冷冻、深蚀刻复制品研究已经揭示了心脏和肝脏间隙连接之间细胞质表面的差异。在胃的表面粘液细胞、小肠和大肠的吸收上皮细胞以及肾脏的远端小管细胞中，与肝脏缝隙连接相似，大多数细胞质表面膜都是单颗粒的。然而，在心房和心室中，主动脉内膜 ...更多信息 这些非上皮间隙连接的纤毛突、胞质表面膜一致地显示出特征性的颗粒图案，如由不同大小的圆形瓦片组成的瓦片表面。仔细检查发现，这些颗粒状材料不仅附着在膜表面，而且作为间隙连接颗粒的组成部分从膜内部突出。在同一复制区域的妊娠大鼠子宫中，我们发现子宫肌层平滑肌缝隙连接的表面膜呈颗粒状，而子宫内膜上皮的表面膜则较为光滑.本研究结果表明，上皮和非上皮间隙连接的胞质表面结构不同，非上皮间隙连接含有上皮来源的差距连接所不含的胞质外表面结构域，而上皮和非上皮组织间的中间丝蛋白分布趋势相似。少

项目成果

Shibata, Y., Izumi, T. and Yamamot, T.: "Tissue specific granularity of gap junction cytoplasmic surface revealed by quick-freeze, deep-etch replicas." The Anatomical Record.

Shibata, Y.、Izumi, T. 和 Yamamot, T.：“通过快速冷冻、深蚀刻复制品揭示间隙连接细胞质表面的组织特异性粒度。”

SHIBATA,YOSABURO: The Anatomical Record.

柴田与三郎：《解剖记录》。

NOGUCHI,Yasuo: The Anatomical Record. (1987)

野口亚索：解剖记录。

IKEDA,Masahiko: Cell and Tissue Research. 248. 297-303 (1987)

池田正彦：细胞和组织研究。

SHIBATA,YOSABURO: The Anatomical Record. 214. 107-112 (1986)

柴田与三郎：《解剖记录》。