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Magnetometric Evaluation of Toxicity of Gallium Arsenide to Lungs and Alveolar Macrophage

砷化镓对肺和肺泡巨噬细胞毒性的磁力评价

基本信息

批准号：
05454220
负责人：
AIZAWA Yoshiharu
金额：
$ 3.84万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

Gallium arsenide (GaAs) has led to the development of electro-optical devices such as light-emitting diodes and semiconductor lasers. Several reports point to pulmonary exposure to GaAs dust as a potential occupational health hazard. In vivo pulmonary toxicity, immuno toxicity and effects on porphyrin metabolism have been demons trated in animals exposed to GaAs.A rapid decrease in the magnetic field after magnetization of the thorax of animals intratracheally instilled with magnetic particles has been noted and designated as "relaxation". This phenomenon indicates a rapid decrease of remanent magnetic field following magnetization of the lungs due to random rotation of phagocytosed iron oxide particles in macrophages. In the first part of study, delayd relaxation and clearance of iron oxide were observed in rabbits exposed to GaAs.In the latter part of study, alveolar macrophages adhered to glass in the bottom of wells were exposed to iron oxide 60mug with or without GaAs20mug/ml in culture medium. Rapid relaxation was observed in the sample of GaAs free medium, whereas relaxation for the specimen with GaAs was delayd. GaAs impaired relaxation dose-dependently. Since addition of Ga_2O_3 into the medium did not affect relaxation, arsenic is considered affecting relaxation.LDH release from cells, classical test for cellular toxicity, also indicated toxic effect of GaAs on macrophages. Transmission electron micros copy revealed alterations of cell membrane, organelles and nucleus in macrophages exposed to GaAs.In vivo cellular magnetometry appears applicable to evaluate toxicity of chemical substance on phagocytic cells.

砷化镓（GaAs）导致了诸如发光二极管和半导体激光器的电光器件的发展。一些报告指出，肺部接触砷化镓粉尘是一种潜在的职业健康危害。本文报道了GaAs对动物的肺毒性、免疫毒性和对卟啉代谢的影响，并观察到在动物胸腔内注入磁性粒子磁化后，磁场迅速下降，称为“弛豫”。这种现象表明，由于巨噬细胞中吞噬的氧化铁颗粒的随机旋转，在肺部磁化后，磁共振磁场迅速下降。本研究的第一部分观察到GaAs对家兔肺泡巨噬细胞的延迟舒张和清除，第二部分观察到粘附在威尔斯孔底玻璃上的肺泡巨噬细胞暴露于含或不含GaAs（20 μ g/ml）的培养液中的氧化铁（60 μ g）。在无GaAs介质的样品中观察到快速弛豫，而在有GaAs介质的样品中观察到延迟弛豫。GaAs对弛豫的影响呈剂量依赖性。由于在培养液中加入Ga_2O_3不影响弛豫，砷被认为影响弛豫.细胞释放LDH，经典的细胞毒性试验，也表明GaAs对巨噬细胞的毒性作用.透射电子显微镜观察到GaAs染毒后巨噬细胞的细胞膜、细胞器和细胞核发生了改变，体内细胞磁测量法可用于评价化学物质对巨噬细胞的毒性。