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Immunological Investigation on the adverse reaction of iodinated contrast media

碘造影剂不良反应的免疫学研究

基本信息

批准号：
05454306
负责人：
KATAYAMA Hitoshi
金额：
$ 3.46万
依托单位：
Juntendo University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454306/
关键词：
Iodinated contrast media Adverse reaction contrast media IgE Interleukin 4 抗原抗体反応

项目摘要

Our investigation during 1993 - 1994, showed a possible adjuvant effect on IgE antibody production in mice. In addition, the adjuvant effect is probably related to the induction immunological reaction of interleukin 4 (reported at contrast media research conference, Turku, Finland, in June 1995 and submitted to the British Journal of Radiology). In 1995, we thought that we should the confirm our previous results mentioned above, and perform a same procedure using nonionic dimeric contrast media, because delayd adverse reaction of nonionic dimeric contrast media became urgent medicosocial or medicolegal problems. The IgE antibody production was again evaluated, using new iodinated contrast media. In addition to iopamidol, we included to varieties of the nonionic dimeric contrast media (iotrolan and iodixanol) in our experiments. BALB/c male mice (8 weeks) are divided into eight experimental groups ; A saline B DNP-KLH + ALUM C DNP-KLH + ALUM + iopamidol D ALUM + iopamidol E DNP-KLM + ALUM + iotrolan F ALUM + iotrolan G DNP-KLH + ALUM + iodixanol H ALUM + iodixanol. Total IgE production was measured at pre-, one week, three weeks, and five weeks after immunization, by using ELISA technique. The results have not shown significant increase of total IgE among the eight experimental groups so far. Data analysis is still being continued.

我们在1993 - 1994年期间的研究表明，可能的佐剂作用的IgE抗体产生的小鼠。此外，佐剂效应可能与白细胞介素4的诱导免疫反应有关（1995年6月在芬兰图尔库的造影剂研究会议上报道，并提交给英国放射学杂志）。1995年，我们认为，我们应该确认我们以前的上述结果，并使用非离子型二聚造影剂进行相同的程序，因为非离子型二聚造影剂的延迟不良反应成为迫切的医学社会或法医学问题。使用新的碘化造影剂再次评价IgE抗体产生。除碘帕醇外，我们还在实验中纳入了各种非离子二聚体造影剂（iotrolan和碘克沙醇）。将BALB B/c雄性小鼠（8周）分成8个实验组：A盐水B DNP-KLH + ALUM C DNP-KLH + ALUM +碘帕醇D ALUM +碘帕醇E DNP-KLM + ALUM +碘曲兰F ALUM +碘曲兰G DNP-KLH + ALUM +碘克沙醇H ALUM+碘克沙醇。用ELISA法测定免疫前、免疫后1周、3周和5周的总IgE产生量。结果显示，到目前为止，八个实验组中的总IgE没有显著增加。数据分析仍在继续。