Immunological Investigation on the adverse reaction of iodinated contrast media

我们在1993 - 1994年间的研究表明，佐剂可能对小鼠的IgE抗体产生有影响。此外，辅助作用可能与白细胞介素4的诱导免疫反应有关（1995年6月在芬兰图尔库的造影剂研究会议上报道，并提交给英国放射学杂志）。1995年，由于非离子二聚体造影剂的迟发性不良反应成为迫切的医学社会或医学法律问题，我们认为应在确认上述结果的基础上，采用非离子二聚体造影剂进行同样的处理。使用新的碘化造影剂再次评估IgE抗体的产生。除了iopamidol，我们还在实验中加入了多种非离子型二聚体造影剂（iotrolan和iodixanol）。BALB/c雄性小鼠（8周）分为8个实验组；A盐水B DNP-KLH +明矾C DNP-KLH +明矾+ iopamidol D明矾+ iopamidol E DNP-KLM +明矾+ iotrolan F明矾+ iotrolan G DNP-KLH +明矾+碘二醇H明矾+碘二醇。免疫前、免疫后1周、免疫后3周、免疫后5周采用ELISA技术测定总IgE产量。到目前为止，8个实验组的总IgE没有明显增加。数据分析仍在继续。

Our investigation during 1993 - 1994, showed a possible adjuvant effect on IgE antibody production in mice. In addition, the adjuvant effect is probably related to the induction immunological reaction of interleukin 4 (reported at contrast media research conference, Turku, Finland, in June 1995 and submitted to the British Journal of Radiology). In 1995, we thought that we should the confirm our previous results mentioned above, and perform a same procedure using nonionic dimeric contrast media, because delayd adverse reaction of nonionic dimeric contrast media became urgent medicosocial or medicolegal problems. The IgE antibody production was again evaluated, using new iodinated contrast media. In addition to iopamidol, we included to varieties of the nonionic dimeric contrast media (iotrolan and iodixanol) in our experiments. BALB/c male mice (8 weeks) are divided into eight experimental groups ; A saline B DNP-KLH + ALUM C DNP-KLH + ALUM + iopamidol D ALUM + iopamidol E DNP-KLM + ALUM + iotrolan F ALUM + iotrolan G DNP-KLH + ALUM + iodixanol H ALUM + iodixanol. Total IgE production was measured at pre-, one week, three weeks, and five weeks after immunization, by using ELISA technique. The results have not shown significant increase of total IgE among the eight experimental groups so far. Data analysis is still being continued.