Expression of cytokines on renal biopsy specimens in patienbts with chronic glomerulonephritis
慢性肾小球肾炎患者肾活检标本细胞因子的表达
基本信息
- 批准号:05454343
- 负责人:
- 金额:$ 3.71万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1993
- 资助国家:日本
- 起止时间:1993 至 1995
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
patients undergoing endstage renal disease are ever increasing in Japan. Chronic glomerulonephritis occupies more than 40% of total patients who were registered in the Japanese Registry of Dialysis Patients in the year of 1994. The aim of this study was to elucidate the role of cytokines in the development and progression of this disease. Expression of mRNA was analyzed by an in situ hybridization technique which was developed in this laboratory. More than 20 subsatances which include cytokines, collagens, collagenases, inhibitors of collagenases, and enzymes related to oxygen radicals were analyzed. Among various cytokines, IL-4, IL-12 and IFN-gamma have been identified as key cytokines in the activation of phagocytes and their production of oxygen radicals in patients with choronic glomenulonephritis, especially in those with IgA nephropathy. There was an interesting contrast between patients with choronic glomerulonephritis and diabetic nephropathy. The formers expressed TGF-beta, IL-6 and collagenases in parallel with the severity of glomenular dagage while the latters showed inverse relationship. It is concluded that some cytokines play key roles in the glomerulosclerosis in patients with chronic glomerulonephritis. Aberrations of collagen metabolism seemed to be disease specific patters. Further studies are warranted to identify the sequence of events whoch is essential in the progress of glomerular damage in patients with chronic glomerulonephritis.
在日本,患有终末期肾病的患者不断增加。1994年,慢性肾小球肾炎占日本透析患者登记处登记的患者总数的40%以上。本研究的目的是阐明细胞因子在这种疾病的发展和进展中的作用。通过本实验室开发的原位杂交技术分析mRNA的表达。分析了20多个亚组,包括细胞因子、胶原、胶原酶、胶原酶抑制剂和与氧自由基相关的酶。在各种细胞因子中,IL-4、IL-12和IFN-γ已被鉴定为在患有脉络膜肾小球肾炎的患者,特别是患有伊加肾病的患者中活化吞噬细胞及其产生氧自由基的关键细胞因子。脉络膜肾小球肾炎和糖尿病肾病患者之间存在有趣的对比。TGF-β、IL-6和胶原酶的表达与肾小球病变的严重程度呈平行关系,而胶原酶的表达与肾小球病变的严重程度呈负相关。提示某些细胞因子在慢性肾小球肾炎肾小球硬化中起重要作用。胶原代谢异常似乎是疾病特异性模式。进一步的研究是必要的,以确定在慢性肾小球肾炎患者肾小球损害的进展过程中的事件顺序。
项目成果
期刊论文数量(38)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Sakai H, Yamauchi F, Yano N, Endoh M, Nomoto Y: "Cellular immunmity in IgA nephroanthy" Nephrology. (in press).
Sakai H、Yamauchi F、Yano N、Endoh M、Nomoto Y:“IgA 肾病中的细胞免疫”肾脏病学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Sakai H,Yamauchi F,Yaro N,Endoh M,Nomoto Y.: "Cellulan immnmity in IgA nephropathy." Nephrology. (in press).
Sakai H,Yamauchi F,Yaro N,Endoh M,Nomoto Y.:“IgA 肾病中的纤维素免疫。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kashem,A,et al: "FcαR expression on polymorphonuclear lenkoyte and superoxide generation in IgA nephropthy" Kidney International. 45. 868-875 (1994)
Kashem,A 等人:“IgA 肾病中多形核细胞的 FcαR 表达和超氧化物生成”《肾脏国际》45. 868-875 (1994)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Sakai h., Maka R., Suzuki D., Nomoto y., Miyazaki M., Nicolicpeterson DJ., Atkins RC: "In sutu hybriclization analysis of TGF-beta in glomeruli from patients with IgA nephropathy" Contribution to Nephrology. 111. 107-115 (1995)
Sakai h.、Maka R.、Suzuki D.、Nomoto y.、Miyazaki M.、Nicolicpeterson DJ.、Atkins RC:“IgA 肾病患者肾小球中 TGF-β 的 In sutu 杂交分析”对肾脏病学的贡献。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Sakai H, Jinde K, Suzuki D, Yagame M, Nomoto Y.: "Molecular approaches to the study ot reual biopsy" Nephrology. (in press).
Sakai H、Jinde K、Suzuki D、Yagame M、Nomoto Y.:“真实活检研究的分子方法”肾病学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
SAKAI Hideto其他文献
SAKAI Hideto的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('SAKAI Hideto', 18)}}的其他基金
Analysis of transcriptome in glomeruli of patients with IgA nephropathy : comprehensive study using high-density cDNA array
IgA肾病患者肾小球转录组分析:利用高密度cDNA芯片的综合研究
- 批准号:
11470220 - 财政年份:1999
- 资助金额:
$ 3.71万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
IgA specific helper cytokines in IgA nephropathy
IgA 肾病中的 IgA 特异性辅助细胞因子
- 批准号:
02670289 - 财政年份:1990
- 资助金额:
$ 3.71万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Study on IgA-specific switch T cells in patients with IgA nephropathy.
IgA肾病患者IgA特异性开关T细胞的研究
- 批准号:
62570300 - 财政年份:1987
- 资助金额:
$ 3.71万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)