Study on IgA-specific switch T cells in patients with IgA nephropathy.

IgA肾病患者IgA特异性开关T细胞的研究

• 批准号: 62570300
• 负责人: SAKAI Hideto
• 金额: $ 1.79万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570300/
• 关键词: IgA nephropathy; Switch T cells; CD4; IgA; IgA産生能亢進; 家族性免疫異常; T_<α4>細胞; IgA産生; IgA産生亢進; ヘルパーT細胞

Enumeration and functional analysis of CD4^+ T cells with receptors for the Fc portion of IgA (i.e. Talpha4 cells) in the peripheral blood or patients with IgA nephropathy, their relatives and age-matched controls were performed to elucidate polyclonal activation of IgA production in this disease. Enumeration of Talpha4 cells was performed by a fluorescence activated cell sorter, and functional analysis was carried out by separation of Talpha4 cells, and IgM-, IgA- and IgG-bearing lymphocytes using panning methods followed by cultures of these cells for 7 days with pokewecd mitogen. There was a significant increase in the amount of peripheral blood Talpha4 cells in patients with IgA nephropathy and their relatives. Talpha4 cells specifically enhanced the switch of IgM-bearing cells to IgA-bearing cells, and this switch activity was inhibited by addition or human myeloma IgA. It is suggested that Talpha4 cells may be responsible for polyclonal activation of IgA production in IgA nephropathy.

对伊加肾病患者、其亲属和年龄匹配的对照者外周血中具有伊加Fc部分受体的CD4^+ T细胞（即Talpha4细胞）进行计数和功能分析，以阐明该疾病中伊加产生的多克隆活化。通过荧光激活细胞分选仪进行Talpha4细胞的计数，并通过使用淘选方法分离Talpha4细胞和携带IgM、伊加和IgG的淋巴细胞，然后用美洲商陆有丝分裂原将这些细胞培养7天来进行功能分析。伊加肾病患者及其亲属外周血中的Talpha4细胞数量显著增加。Talpha4细胞特异性地增强了携带IgM的细胞向携带IgA的细胞的转换，并且这种转换活性被添加或人骨髓瘤伊加抑制。这表明，在伊加肾病中，Talpha4细胞可能负责伊加产生的多克隆激活。

项目成果

Sakai,H.: In: Clarkson, A.R., ed., IgA Nephropathy,Marthinus Nijhoff Publishing,Boston,Dordrecht and Lancaster. 176-187 (1987)

Sakai, H.：见：Clarkson, A.R. 编辑，IgA 肾病，Marthinus Nijhoff 出版社，波士顿、多德雷赫特和兰开斯特。

Sakai,H.(分担執筆): "Recent Studies of IgA Nephropathy in Japan" Nishimura Co.,Ltd./Smith-Gordon and Co.,Ltd., 21 (1989)

Sakai, H.（撰稿人）：“日本 IgA 肾病的最新研究” Nishimura Co., Ltd./Smith-Gordon and Co., Ltd., 21 (1989)

Sakai, H.: "Cellular immunoregulatory aspects of IgA nephropathy." Amer. J. Kidney Dis. 12:430-432, 1988.

Sakai, H.：“IgA 肾病的细胞免疫调节方面。”

Sakai,H.(分担執筆): "International Yearbook of Nephrology 1989" Kluwer Academic Publishers, 24 (1989)

Sakai, H.（撰稿人）：《1989 年国际肾病学年鉴》Kluwer 学术出版社，24 (1989)

Sakai, H., Miyazaki, M., Endo, M. and Nomoto, Y.: "Increase of IgA-specific switch T cells in patients with IgA nephropathy." Clin. Exp. Immunol. 78:378-382, 1989.

Sakai, H.、Miyazaki, M.、Endo, M. 和 Nomoto, Y.：“IgA 肾病患者 IgA 特异性开关 T 细胞的增加。”