Analysis of transcriptome in glomeruli of patients with IgA nephropathy : comprehensive study using high-density cDNA array

IgA肾病患者肾小球转录组分析：利用高密度cDNA芯片的综合研究

• 批准号: 11470220
• 负责人: SAKAI Hideto
• 金额: $ 9.6万
• 依托单位: Tokai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470220/
• 关键词: renal biopsy; microarray; transcriptome; genomics; IgA nephropathy; IgA nephropathy; IgA腎症; cDNA アレイ; 糸球体; 遺伝子; CDNA array; 腎生検; メサンギウム細胞; 硬化; 尿細管上皮

A comprehensive profile of genes expressed at the mRNA level (transcriptome) in human renal tissue is important for elucidating the pathogenesis and treatment of IgA nephropathy. The recent development of cDNA array hybridization allows the parallel monitoring of thousands of genes expressed in renal tissue. High-density CDNA array containing 18, 326 paired unique human cDNA gene probes were used to quantitatively analyze the expression of genes in renal biopsies of IgA nephropathy (IgAN) and control. Computational analysis was used to cluster genes according to similarity in pattern of gene expression. Through relational database analysis, we determined 1,901 genes that were commonly expressed in selected IgAN renal biopsies and 4 controls. Further analysis of the expressed genes by self-organizing maps and hierarchical clustering revealed a genomic profile unique to severely affected IgAN patients. These findings represent a comprehensive preliminary molecular index of genes transcrebed in IgA nephropathy. More importantly, this molecular approach may accelerate the discovery of pathogenic mechanisms underlying the worldwide most common form of glomerulonephritis.

人类肾组织中mRNA水平（转录组）表达的基因的全面概况对于阐明伊加肾病的发病机制和治疗非常重要。最近发展的cDNA阵列杂交允许平行监测成千上万的基因表达在肾组织。应用含有18，326对独特的人cDNA基因探针的高密度cDNA阵列对伊加肾病（IgAN）和对照肾活检组织中的基因表达进行定量分析。根据基因表达模式的相似性，使用计算分析对基因进行聚类。通过相关数据库分析，我们确定了1，901个在选定的IgAN肾活检和4个对照中常见表达的基因。通过自组织图谱和层次聚类对表达基因的进一步分析揭示了严重影响IgAN患者的独特基因组谱。这些发现代表了一个全面的初步分子指标的基因transscrebed在伊加肾病。更重要的是，这种分子方法可能会加速发现全球最常见的肾小球肾炎的致病机制。

项目成果

Yano.N.: "Comprehensive gene expression profile of the adult human renal cortex:analysis by cDNA array hybridization."Kidney International. 4(in press). (2000)

Yano.N.：“成人肾皮质的综合基因表达谱：通过 cDNA 阵列杂交进行分析。”肾脏国际。

Yano, N., Endoh, M., Fadden K.J., Yamashita, H., Sakai, H., Kurokawa, K., Abboud, H.E., Rifai, A.: "Genomic repertoire of human mesangial cells: comprehensive analysis of gene expression by cDNA array hybridization"Nephrology. 5. 215-223 (2000)

Yano, N.、Endoh, M.、Fadden K.J.、Yamashita, H.、Sakai, H.、Kurokawa, K.、Abboud, H.E.、Rifai, A.：“人类系膜细胞的基因组库：基因表达的综合分析

YANO, N., Endoh, M., Fadden K., Yamashita, H., Kane, A., Sakai, H., Raifai, A.: "Comprehensive gene expression profile of the adult human renal cortex : Analysis by cDNA array hybridization"Kidney Infernational. 57. 1452-1459 (2000)

YANO, N.、Endoh, M.、Fadden K.、Yamashita, H.、Kane, A.、Sakai, H.、Raifai, A.：“成人肾皮质的综合基因表达谱：通过 cDNA 阵列进行分析

Yano.N.: "Comprehensive gene expression profile of the abult human renal cortex : analysis by cDNA array hybridization."Kidney International. 4. (2000)

Yano.N.：“人肾皮质的综合基因表达谱：通过 cDNA 阵列杂交进行分析。”肾脏国际。

Yano, N., Endoh, M., Fadden KJ, et al.: "Genomic repertoire of human mesangial cells : comprehensive analysis of gene expression by cDNA array hybridization"Nephrology. 5・4. 215-223 (2000)

Yano, N.、Endoh, M.、Fadden KJ 等：“人类系膜细胞的基因组库：通过 cDNA 阵列杂交对基因表达进行综合分析”Nephrology 5·4 (2000)。