Elucidation of the autonomic nervous control of immune organs.

本文观察了非伤害性和伤害性机械刺激对麻醉大鼠脾NK细胞杀伤活性和脾血流量的影响，结果表明，双侧刷胸至后肢体表30 min对脾NK细胞杀伤活性无明显影响，结果表明，双侧夹捏后爪皮肤30 min可使脾NK细胞杀伤活性和脾血流量分别降低至对照值的<plus-minus>67.8%和82.3%<plus-minus>，而夹捏后爪皮肤30 min可使脾交感传出神经活性增加<plus-minus>143.15%电刺激内脏神经可引起脾NK细胞毒活性和脾血流量的降低，而后爪伤害性捏夹可引起脾交感神经或脊髓的抑制。这些结果表明，后爪伤害性捏夹刺激后NK细胞毒活性和脾血流量的代谢是一种超负性代谢。脊髓反射通过脾交感神经介导，而腹部挤压30 min则可降低中枢神经系统完整和脊髓损伤大鼠脾NK细胞的细胞毒活性，因此腹部刺激可通过脊髓反射抑制NK细胞的活性。

The effects of non-noxious and noxious mechanical stimulation of the skin on cytotoxic activity of splenic natural killer (NK) cells and splenic blood flow were examined in anesthetized rats.Bilateral brushing of the body surface between the lateral chest and hindlimb for 30 min did not significantly influence cytotoxic activity of splenic NK cells, splenic blood flow or splenic sympathetic efferent nerve activity.Bilateral pinching stimulation of the skin of the hindpaws for 30 min reduced cytotoxic activity of splenic NK cells and splenic blood flow to 67 <plus-minus> 8 and 82 <plus-minus> 3% of the control values, respectively.Pinching hindpaws for 30 min increased splenic sympathetic efferent nerve activity ton 143 <plus-minus> 15% of control.Electrical stimulation of the splanchnic nerve produced a decrease in the cytotoxic activity of splenic NK cells and splenic blood flow.The hindpaw pinching-induced suppression of the splenic sympathetic nerve or the spinal cord at the cervical level.These results indicate that the supersession of cytotoxic activity of NK cells and splenic blood flow following noxious pinching stimulation of hindpaws is a supra-spinal reflex response mediated via the splenic sympathetic nerve.On the other hand, pinching of abdomen for 30 min reduced cytotoxic activity of splenic NK cells both in the central nervous system intact and spinalized rats.Therefore, the abdominal stimulation suppressed the NK activity via spinal reflex pa