A development of a new method for continuous measurement of regional cerebral blood flow and analysis of the regulation of cerebral blood flow in a conscious rat.

开发一种连续测量清醒大鼠局部脑血流并分析脑血流调节的新方法。

• 批准号: 06558111
• 负责人: SATO Akio
• 金额: $ 9.92万
• 依托单位: Tokyo Metropolitan Institute of Gerontology (TMIG)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06558111/
• 关键词: cerebral blood flow; nerotransmitter; conscious animals

1.We have developed a new system to continuously measure regional blood flow (rCBF) in the cortex of a conscious rat using laser Doppler flowmetry.Under anesthesia, the animal's skull was opened making a small square hole and a transparent acrylic plate was placed over the hole.A polyethylene cannula was fixed on the plate as a guide for the laser Doppler flowmeter (LDF) probe.Both the plate and guide cannula were fixed to the skull by dental cement.After recovery of anesthesia, the conscious animal was placed in a hammock with their legs firmly touching the floor.This system is recommended for the continuous measurement of rCBF in a conscious animal.Using this method, rCBF in conscious rats was continuously measured during walking.rCBF in the frontal, parietal, and occipital corticies increased during walking at a mild speed (4cm/s) for a period of 30 s.The responses of rCBF in the three corticies were almost identical.Walking-induced increase in cortical rCBF was partially reduced by systemic administration of both blood brain barrior (BBB) -permeable muscarinic and nicotinic cholinergic receptor antagonists, but was unaffected by BBB-impermeable drugs.This suggests the involvement of both muscarinic and nicotinic receptors in the rCBF responses in the central nervous system.We could also demonstrate that extracellular acetylcholine in the cerebral cortex increased during walking at a mild speed in conscious rats.Circumstantially, these data suggest that the walking-induced increase in cortical rCBF is, at least in part, to activation of the cholinergic vasodilative system in the cortex originating in the nucleus basalis of Meynert.

1.我们建立了一种新的激光多普勒血流仪连续测量清醒大鼠大脑皮层局部血流量（rCBF）的系统。将动物的头骨打开，开一个小的方孔，在孔上放置一个透明的丙烯酸板，在板上固定一个聚乙烯套管作为激光多普勒流量计（LDF）的导向器。探针。用牙科粘固剂将板和引导套管固定在颅骨上。麻醉恢复后，将清醒的动物置于吊床上，使其腿牢固地接触地板。该系统被推荐用于清醒动物的rCBF的连续测量。使用该方法，在行走过程中连续测量清醒大鼠的rCBF。额叶，顶叶，以4cm/s的速度步行30 s时，枕叶皮质和小脑皮质的rCBF反应基本一致，但在步行过程中，枕叶皮质和小脑皮质的rCBF反应基本一致。通过全身施用血脑屏障（BBB）-可渗透的毒蕈碱和烟碱胆碱能受体拮抗剂，但不受BBB渗透性药物的影响。这表明在中枢神经系统的rCBF反应中，毒蕈碱和烟碱受体都参与其中。我们还可以证明，清醒大鼠在以温和速度行走时，大脑皮层中的细胞外乙酰胆碱增加。在这种情况下，这些数据表明，行走诱导的皮质rCBF的增加至少部分是源于Meynert基底核的皮质中胆碱能血管舒张系统的激活。

项目成果

Sato, A.et al.: "A new method for continuous measurement of regional cerebral blood flow using laser Doppler flowmetry in a conscious rat." Neurosci.Lett.175. 149-152 (1994)

Sato, A.等人：“一种使用激光多普勒血流计连续测量清醒大鼠局部脑血流的新方法。”

Wada, H. et al.: "Kynurenine specifically increases in the cerebrospinal fluid of the aged rats." Biogenic Amines. 10. 221-225 (1994)

Wada, H. 等人：“老年大鼠脑脊液中的犬尿氨酸特别增加。”

Sato, A. et al.: "Cholinergic neural regulation of regional cerebral blood flow." Alzheimer Disease and Associated Disorders. 9. 28-38 (1995)

Sato, A. 等人：“区域脑血流的胆碱能神经调节。”

Sato A. et al.: "Blood flow in the sciatic nerveis regulated by vasoconstirictive and vasodilative nerve fibers originating from the ventral and dorsal roots of the spinal nerves." Neurosci. Res.21. 125-133 (1994)

Sato A. 等人：“坐骨神经中的血流受到源自脊神经腹侧和背侧根的血管收缩和血管舒张神经纤维的调节。”

Kimura, A. et al.: "Regional cerebral blood flow in the frontal, parietal and occipital cortices increases independently of systemic arterial pressure during slow walking in conscious rats." Neurosci. Res.20. 309-315 (1994)

Kimura, A. 等人：“在清醒的大鼠缓慢行走过程中，额叶、顶叶和枕叶皮质的区域脑血流量增加，与全身动脉压无关。”